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| Registros recuperados : 55 | |
| 4. |  | MARTINS, C. F.; BÁO, S. N.; DODE, M. N.; RUMPF, R. Avaliação ultra-estrutural e da fragmentação do dna de espermatozóides bovinos conservados por diferentes tratamentos de liofilização. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 10., 2005, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 91.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | MARTINS, C. F.; BÁO, S. N.; DODE, M. N.; CORREA, G. A.; RUMF, R. Effects of freeze-drying on cytology, ultrastructure, DNA fragmentation, and fertilizing ability of bovine sperm. Theriogenology, v. 67, p. 1307-1315, 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | MARTINS, C. F.; DODE, M. N.; BÁO, S. N.; RUMPF, R. Utilização do teste com acridina laranja e túnel para avaliar a integridade dos espermatozóides liofilizados de bovinos. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 110.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | SILVA, C. G.; CUNHA, E. R.; BLUME, G. R.; MALAQUIAS, J. V.; BÁO, S. N.; MARTINS, C. F. Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose. Cryobiology, v. 70, p. 90-94, 2015. p. 90-94| Biblioteca(s): Embrapa Cerrados. |
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| 13. | | CUNHA, E. R. da; MARTINS, C. F.; SILVA, G. C.; CUMPA, H. C. B.; BAO, S. N. Effects of prolonged in vitro culture and cryopreservation on viability, DNA Fragmentation, chromosome stability and ultrastructure of bovine cells from amniotic fluid and umbilical cord. Reproduction in Domestic Animals, v. 49, n. 5, p. 806-812, Oct. 2014.| Biblioteca(s): Embrapa Cerrados. |
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| 16. | | ARDISSON-ARAÚJO, D. M. P.; MELO, F. L.; SIHLER, W.; RIBEIRO, B. M.; BÁO, S. N.; SOUZA, M. L. de. Genome organization of Erinnyis ello granulovirus (EeGV), an efficient biopesticide used in Brazilian cassava crops. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 13., 2013, Bonito, MS. Faça bonito: use controle biológico: anais. Brasília, DF: Embrapa, 2013.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 18. | | SILVEIRA, E. B. da; CORDEIRO, B. A.; RIBEIRO, B. M.; CASTRO, M. E. B. de C.; SOARES, E. F.; BÁO, S. N. An Anticarsia gemmatalis multiple nucleopolyhedrovirus mutant, v. ApAg, induces hemocytes apoptosis in vivo and displays reduced infectivity in larvae of Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae). Virus Research, v. 130, p. 182-192, 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 19. | | SILVA, C. G. da; MARTINS, C. F.; CARDOSO, T. C.; CUNHA, E. R. da; CUMPA, H. C. B.; MCMANUS, C. M.; PIVATO, I.; BAO, S. N. Isolation and characterization of mesenchymal stem cells derived from bovine Wharton's jelly and their potential for use in cloning by nuclear transfer. Isolamento e caracterização de células tronco mesenquimais derivadas de geléia de Wharton bovina e seu potencial para uso na clonagem por transferência nuclear. Ciência Rural, Santa Maria, v. 46, n. 10, p. 1830-1837, out. 2016.| Biblioteca(s): Embrapa Cerrados. |
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| 20. | | RICARTE, A. R. F.; TEIXEIRA, M. F. da S.; ANDRIOLI, A.; PINHEIRO, R. R.; BÁO, S. N.; SILVA, J. B. A. da. Análise molecular e ultraestrutural de espermatozóides caprinos oriundos de animais infectados naturalmente e experimentalmente com o CAEV. In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 6.; SIMPÓSIO NORDESTINO DE ALIMENTAÇÃO DE RUMINANTES, 7.; FÓRUM DE COORDENADORES DE PÓS GRADUAÇÃO EM PRODUÇÃO ANIMAL DO NORDESTE, 1.; FÓRUM DE AGROECOLOGIA RO RIO GRANDE DO NORTE, 1., 2010, Mossoró. Anais... Mossoró: Sociedade Nordestina de Producao Animal; UFERSA, 2010. 4 f. 1 CD-ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 55 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
30/09/2008 |
|
Data da última atualização: |
03/02/2009 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
PARENTE, A. F.; SILVA-PEREIRA, I.; BALDANI, J. I.; TIBÚRCIO, V. H. da S.; BÁO, S. N.; DE SOUZA, M. T. |
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Afiliação: |
Ana Flávia Parente, UnB; Ildinete Silva-Pereira, UnB; José Ivo Baldani, Embrapa Agrobiologia; Victor Hugo da Silva Tibúrcio, UnB; Sônia Nair Báo, UnB; Marlene T. de Souza, UnB. |
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Título: |
Construction of Bacillus thuringiensis wild-type S76 and Cry- derivatives expressing a green fluorescent protein: two potential marker organisms to study bacteria-plant interactions. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Canadian Journal of Microbiology, Ottawa, v. 54, p. 786-790, 2008. |
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Idioma: |
Inglês |
|
Notas: |
Parceria: UnB. |
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Conteúdo: |
Collectively, the species Bacillus thuringiensis, Bacillus cereus, and Bacillus anthracis represent microorganisms of high economic, medical, and biodefense importance, Although the genetic correlation and pathogenic characteris-tics have been extensively dissected, the ecological properties of these three species in their natural environments remain poorly understood, Thus, a tractable marker for detecting these bacteria under specific environmental and physiological conditions is a valuable tool. With this purpose, a plasmid (pAD43-25) carrying a functional gfp gene sequence (gfpmut3A) was introduced into the wild-type strain Bacillus thuringiensis subsp. kurslaki S76, which bears approximately 11 plasmids, allowing constitutive synthesis of green fluorescent protein (GFP) during vegetative growth (strain S76GFP+). Additionally, this vector was transferred to a plasmid-cured (Cry-) B. thuringiensis host. Bright green cells were detected by fluorescence microscopy in both recombinants by 2 h after inoculation in liquid medium and could be seen throughout the remaining cultivation time until complete sporulation was accomplished. For strain S76GFP+ protein profile and plasmid DNA analyses indicate, respectively, that this recombinant maintained Cry proteins expression and resident plasmid outline. Thus, in addition to the potential of strain S76GFP+ as a marker organism in bacteria-plant interaction studies, the production and stability of active GFPmut3a make this unique expression system a useful experimental model to study adaptive changes of host-plasmid as well as plasmid-plasmid relationships in a population of cells stressed by the production of a recombinant protein. MenosCollectively, the species Bacillus thuringiensis, Bacillus cereus, and Bacillus anthracis represent microorganisms of high economic, medical, and biodefense importance, Although the genetic correlation and pathogenic characteris-tics have been extensively dissected, the ecological properties of these three species in their natural environments remain poorly understood, Thus, a tractable marker for detecting these bacteria under specific environmental and physiological conditions is a valuable tool. With this purpose, a plasmid (pAD43-25) carrying a functional gfp gene sequence (gfpmut3A) was introduced into the wild-type strain Bacillus thuringiensis subsp. kurslaki S76, which bears approximately 11 plasmids, allowing constitutive synthesis of green fluorescent protein (GFP) during vegetative growth (strain S76GFP+). Additionally, this vector was transferred to a plasmid-cured (Cry-) B. thuringiensis host. Bright green cells were detected by fluorescence microscopy in both recombinants by 2 h after inoculation in liquid medium and could be seen throughout the remaining cultivation time until complete sporulation was accomplished. For strain S76GFP+ protein profile and plasmid DNA analyses indicate, respectively, that this recombinant maintained Cry proteins expression and resident plasmid outline. Thus, in addition to the potential of strain S76GFP+ as a marker organism in bacteria-plant interaction studies, the production and stability of active GFPmut3a make this unique e... Mostrar Tudo |
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Palavras-Chave: |
Cana-de-açúcar. |
|
Thesagro: |
Bacillus Thuringiensis; Controle Biológico; Diatraea Saccharalis. |
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Categoria do assunto: |
-- |
|
Marc: |
LEADER 02517naa a2200241 a 4500
001 1598585
005 2009-02-03
008 2008 bl --- 0-- u #d
100 1 $aPARENTE, A. F.
245 $aConstruction of Bacillus thuringiensis wild-type S76 and Cry- derivatives expressing a green fluorescent protein$btwo potential marker organisms to study bacteria-plant interactions.
260 $c2008
500 $aParceria: UnB.
520 $aCollectively, the species Bacillus thuringiensis, Bacillus cereus, and Bacillus anthracis represent microorganisms of high economic, medical, and biodefense importance, Although the genetic correlation and pathogenic characteris-tics have been extensively dissected, the ecological properties of these three species in their natural environments remain poorly understood, Thus, a tractable marker for detecting these bacteria under specific environmental and physiological conditions is a valuable tool. With this purpose, a plasmid (pAD43-25) carrying a functional gfp gene sequence (gfpmut3A) was introduced into the wild-type strain Bacillus thuringiensis subsp. kurslaki S76, which bears approximately 11 plasmids, allowing constitutive synthesis of green fluorescent protein (GFP) during vegetative growth (strain S76GFP+). Additionally, this vector was transferred to a plasmid-cured (Cry-) B. thuringiensis host. Bright green cells were detected by fluorescence microscopy in both recombinants by 2 h after inoculation in liquid medium and could be seen throughout the remaining cultivation time until complete sporulation was accomplished. For strain S76GFP+ protein profile and plasmid DNA analyses indicate, respectively, that this recombinant maintained Cry proteins expression and resident plasmid outline. Thus, in addition to the potential of strain S76GFP+ as a marker organism in bacteria-plant interaction studies, the production and stability of active GFPmut3a make this unique expression system a useful experimental model to study adaptive changes of host-plasmid as well as plasmid-plasmid relationships in a population of cells stressed by the production of a recombinant protein.
650 $aBacillus Thuringiensis
650 $aControle Biológico
650 $aDiatraea Saccharalis
653 $aCana-de-açúcar
700 1 $aSILVA-PEREIRA, I.
700 1 $aBALDANI, J. I.
700 1 $aTIBÚRCIO, V. H. da S.
700 1 $aBÁO, S. N.
700 1 $aDE SOUZA, M. T.
773 $tCanadian Journal of Microbiology, Ottawa$gv. 54, p. 786-790, 2008.
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