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| Registros recuperados : 55 | |
| 6. |  | MARTINS, C. F.; BÁO, S. N.; DODE, M. N.; CORREA, G. A.; RUMF, R. Effects of freeze-drying on cytology, ultrastructure, DNA fragmentation, and fertilizing ability of bovine sperm. Theriogenology, v. 67, p. 1307-1315, 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | MARTINS, C. F.; BÁO, S. N.; DODE, M. N.; RUMPF, R. Avaliação ultra-estrutural e da fragmentação do dna de espermatozóides bovinos conservados por diferentes tratamentos de liofilização. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 10., 2005, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 91.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | MARTINS, C. F.; DODE, M. N.; BÁO, S. N.; RUMPF, R. Utilização do teste com acridina laranja e túnel para avaliar a integridade dos espermatozóides liofilizados de bovinos. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 11., 2006, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2006. p. 110.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | CUNHA, E. R. da; MARTINS, C. F.; SILVA, G. C.; CUMPA, H. C. B.; BAO, S. N. Effects of prolonged in vitro culture and cryopreservation on viability, DNA Fragmentation, chromosome stability and ultrastructure of bovine cells from amniotic fluid and umbilical cord. Reproduction in Domestic Animals, v. 49, n. 5, p. 806-812, Oct. 2014.| Biblioteca(s): Embrapa Cerrados. |
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| 13. | | ARDISSON-ARAÚJO, D. M. P.; MELO, F. L.; SIHLER, W.; RIBEIRO, B. M.; BÁO, S. N.; SOUZA, M. L. de. Genome organization of Erinnyis ello granulovirus (EeGV), an efficient biopesticide used in Brazilian cassava crops. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 13., 2013, Bonito, MS. Faça bonito: use controle biológico: anais. Brasília, DF: Embrapa, 2013.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 14. | | SILVA, C. G.; CUNHA, E. R.; BLUME, G. R.; MALAQUIAS, J. V.; BÁO, S. N.; MARTINS, C. F. Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose. Cryobiology, v. 70, p. 90-94, 2015. p. 90-94| Biblioteca(s): Embrapa Cerrados. |
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| 18. | | SILVA, C. G. da; MARTINS, C. F.; CARDOSO, T. C.; CUNHA, E. R. da; CUMPA, H. C. B.; MCMANUS, C. M.; PIVATO, I.; BAO, S. N. Isolation and characterization of mesenchymal stem cells derived from bovine Wharton's jelly and their potential for use in cloning by nuclear transfer. Isolamento e caracterização de células tronco mesenquimais derivadas de geléia de Wharton bovina e seu potencial para uso na clonagem por transferência nuclear. Ciência Rural, Santa Maria, v. 46, n. 10, p. 1830-1837, out. 2016.| Biblioteca(s): Embrapa Cerrados. |
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| 19. | | SILVEIRA, E. B. da; CORDEIRO, B. A.; RIBEIRO, B. M.; CASTRO, M. E. B. de C.; SOARES, E. F.; BÁO, S. N. An Anticarsia gemmatalis multiple nucleopolyhedrovirus mutant, v. ApAg, induces hemocytes apoptosis in vivo and displays reduced infectivity in larvae of Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae). Virus Research, v. 130, p. 182-192, 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | RICARTE, A. R. F.; TEIXEIRA, M. F. da S.; ANDRIOLI, A.; PINHEIRO, R. R.; BÁO, S. N.; SILVA, J. B. A. da. Análise molecular e ultraestrutural de espermatozóides caprinos oriundos de animais infectados naturalmente e experimentalmente com o CAEV. In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 6.; SIMPÓSIO NORDESTINO DE ALIMENTAÇÃO DE RUMINANTES, 7.; FÓRUM DE COORDENADORES DE PÓS GRADUAÇÃO EM PRODUÇÃO ANIMAL DO NORDESTE, 1.; FÓRUM DE AGROECOLOGIA RO RIO GRANDE DO NORTE, 1., 2010, Mossoró. Anais... Mossoró: Sociedade Nordestina de Producao Animal; UFERSA, 2010. 4 f. 1 CD-ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 55 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
11/12/2020 |
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Data da última atualização: |
11/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
ARANTES, L. G.; TONELLI, G. S. S. S.; MARTINS, C. F.; BÁO, S. N. |
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Afiliação: |
CARLOS FREDERICO MARTINS, CPAC. |
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Título: |
Cellular Characterization and Effectsof Cryoprotectant Solutions on the Viabilityof Fibroblasts from Three Brazilian Wild Cats. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Biopreservation and Biobanking, 2020. |
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Idioma: |
Português |
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Conteúdo: |
Preserving genetic material in cryogenic conditions presents a viable alternative for the protection of species' gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree?). Further testing was conducted to determine each solution's performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating intense cell activity after thawing. Differences found in the efficiency of cryopreservation protocols according to the type of cryoprotectant indicate that species react differently to freezing and thawing processes. This research evaluates key aspects of in vitro protocols for cryopreservation of wild animals, which need to be optimized to guarantee successful cell culturing. More suitable protocols lead to increased efficiency in establishing fibroblast cryobanks and also facilitating the use of wild cats' cells in cloning techniques, contributing directly to preserving wild fauna. MenosPreserving genetic material in cryogenic conditions presents a viable alternative for the protection of species' gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree?). Further testing was conducted to determine each solution's performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating... Mostrar Tudo |
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Palavras-Chave: |
Material genético; Protocolo. |
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Thesagro: |
Criopreservação. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02694naa a2200193 a 4500
001 2128006
005 2020-12-11
008 2020 bl uuuu u00u1 u #d
100 1 $aARANTES, L. G.
245 $aCellular Characterization and Effectsof Cryoprotectant Solutions on the Viabilityof Fibroblasts from Three Brazilian Wild Cats.$h[electronic resource]
260 $c2020
520 $aPreserving genetic material in cryogenic conditions presents a viable alternative for the protection of species' gene variability. However, there is an enormous need to establish and test cryopreservation protocols that are suitable for each diverse cell type to guarantee technical success in the long run. Considering this, fibroblasts from jaguar (Panthera onca), oncilla (Leopardus tigrinus), and pampas cat (Leopardus colocolo) were subjected to cell characterization and then cryopreservation in different cryoprotectant solutions (2.5%, 10% dimethyl sulfoxide [DMSO] or CryoSOfree?). Further testing was conducted to determine each solution's performance in preserving cell viability. In culture, a growth curve to assess cellular growth potential showed that exponential proliferation lasts for about the first 50 hours of in vitro culturing, declining in pace afterward. L. colocolo and L. tigrinus presented no difference in cell viability while using 2.5% DMSO protocols. P. onca cells did not present difference on viability for both concentrations of DMSO. Protocols using CryoSOfree resulted in a decreased viability of P. onca fibroblasts. Morphological differences between fibroblasts among the species were noted under bright field microscopy and scanning electron microscopy. L. colocolo and P. onca cells are fusiform, and L. tigrinus are spherical. All cells presented cytoplasmic projections. Transmission electron microscopy revealed vacuoles and secretion granules, indicating intense cell activity after thawing. Differences found in the efficiency of cryopreservation protocols according to the type of cryoprotectant indicate that species react differently to freezing and thawing processes. This research evaluates key aspects of in vitro protocols for cryopreservation of wild animals, which need to be optimized to guarantee successful cell culturing. More suitable protocols lead to increased efficiency in establishing fibroblast cryobanks and also facilitating the use of wild cats' cells in cloning techniques, contributing directly to preserving wild fauna.
650 $aCriopreservação
653 $aMaterial genético
653 $aProtocolo
700 1 $aTONELLI, G. S. S. S.
700 1 $aMARTINS, C. F.
700 1 $aBÁO, S. N.
773 $tBiopreservation and Biobanking, 2020.
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