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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
12/12/2019 |
Data da última atualização: |
12/12/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GIGLIOTI, R.; BASSETO, C. C.; OKINO, C. H.; OLIVEIRA, H. N. de; OLIVEIRA, M. C. de S. |
Afiliação: |
Rodrigo Giglioti, UNESP; César Cristiano Bassetto, UNESP; CINTIA HIROMI OKINO, CPPSE; Henrique Nunes de Oliveira, UNESP; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
Título: |
Development of a loop-mediated isothermal amplification (LAMP) assay for the detection of Anaplasma marginale. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Experimental and Applied Acarology, v. 77, n. 1, p.65?72, 2019. |
Idioma: |
Inglês |
Conteúdo: |
Parasitemia generated by Anaplasma marginale causes significant losses in the cattle industry. A major constraint to the effective control and management of anaplasmosis in livestock is the lack of a rapid and reliable diagnostic test to identify the parasite and allow for immediate therapy. In the present study, we developed a novel DNA-based assay for the detection of A. marginale in bovine blood samples, using loop-mediated isothermal amplification (LAMP). DNA from six cattle and hemoparasite samples (Babesia bovis, Babesia bigemina, Anaplasma centrale and A. marginale) were tested for specificity, sensitivity and cross-reactions. The developed LAMP procedures were also confirmed and compared with the qPCR method. The same gene sequence (major surface protein 1b, msp1b) of A. marginale was used to design a set of primers for the LAMP and qPCR assays. The results showed that LAMP is specific, as no positive signal was observed for the other tested hemoparasites. However, the sensitivity of the qPCR assay was ten times higher than LAMP. Our findings indicate that this LAMP method has a good sensitivity and high specificity for the detection of A. marginale and may have a potential application in the detection and differentiation of bovine anaplasmosis. |
Palavras-Chave: |
Hemoparasita; Hemoparasite; Parasita; Parasite. |
Thesagro: |
Babesia Bigemina; Babesia Bovis. |
Thesaurus Nal: |
Animal parasites and pests; Bovine anaplasmosis; Parasites. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02101naa a2200277 a 4500 001 2116785 005 2019-12-12 008 2019 bl uuuu u00u1 u #d 100 1 $aGIGLIOTI, R. 245 $aDevelopment of a loop-mediated isothermal amplification (LAMP) assay for the detection of Anaplasma marginale.$h[electronic resource] 260 $c2019 520 $aParasitemia generated by Anaplasma marginale causes significant losses in the cattle industry. A major constraint to the effective control and management of anaplasmosis in livestock is the lack of a rapid and reliable diagnostic test to identify the parasite and allow for immediate therapy. In the present study, we developed a novel DNA-based assay for the detection of A. marginale in bovine blood samples, using loop-mediated isothermal amplification (LAMP). DNA from six cattle and hemoparasite samples (Babesia bovis, Babesia bigemina, Anaplasma centrale and A. marginale) were tested for specificity, sensitivity and cross-reactions. The developed LAMP procedures were also confirmed and compared with the qPCR method. The same gene sequence (major surface protein 1b, msp1b) of A. marginale was used to design a set of primers for the LAMP and qPCR assays. The results showed that LAMP is specific, as no positive signal was observed for the other tested hemoparasites. However, the sensitivity of the qPCR assay was ten times higher than LAMP. Our findings indicate that this LAMP method has a good sensitivity and high specificity for the detection of A. marginale and may have a potential application in the detection and differentiation of bovine anaplasmosis. 650 $aAnimal parasites and pests 650 $aBovine anaplasmosis 650 $aParasites 650 $aBabesia Bigemina 650 $aBabesia Bovis 653 $aHemoparasita 653 $aHemoparasite 653 $aParasita 653 $aParasite 700 1 $aBASSETO, C. C. 700 1 $aOKINO, C. H. 700 1 $aOLIVEIRA, H. N. de 700 1 $aOLIVEIRA, M. C. de S. 773 $tExperimental and Applied Acarology$gv. 77, n. 1, p.65?72, 2019.
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5. | | ALMEIDA, J. D. de; MOTTA, I. de O.; VIDAL, L. de A.; BÍLIO, J. F.; PUPE, J. M.; VEIGA, A. D.; CARVALHO, C. H. S. de; LOPES, R. B.; ROCHA, T. L.; SILVA, L. P. da; PIJOL-LUZ, J. R. P.; FREIRE, E. V. S. A. Bicho mineiro (Leucoptera coffeella): uma revisão sobre o inseto e perspectivas para o manejo da praga. Brasília: Embrapa Recursos Genéticos e Biotecnologia, 2020. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 372).Biblioteca(s): Embrapa Café; Embrapa Recursos Genéticos e Biotecnologia. |
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