Data/hora: 21/06/2024 11:21:14

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 31/05/2016
Data da última atualização: | 29/12/2017
Autoria: | FARIAS, T. H. V.; PEREIRA, N. L.; PÁDUA, S. B. de; ALVES, L. de O.; SAKABE, R.; BELO, M. A. de A.; PILARSKI, F.
Afiliação: | THAÍS HELOISA VAZ FARIAS, CAUnesp; NYCOLAS LEVY PEREIRA, CAUnesp; SANTIAGO BENITES DE PÁDUA, AquiVet Saúde Aquática; LINDOMAR DE OLIVEIRA ALVES, CAUnesp; ROBERSON SAKABE, UFF; MARCO ANTONIO DE ANDRADE BELO, UNESP; FABIANA PILARSKI, CAUnesp.
Título: | Na2EDTA anticoagulant impaired blood samples from the teleost Piaractus mesopotamicus.
Ano de publicação: | 2016
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 5, p. 431-435, maio. 2016.
Idioma: | Inglês
Conteúdo: | The present study aimed to evaluate the effects of Na heparin and Na2EDTA on blood of Piaractus mesopotamicus (360.7±42.4g, 26.4±1.0cm). Twenty fishes were sampled in two experiment trials, ten for erythrocyte fragility analysis and ten for hematologic and plasma biochemical study. The blood collected by venous-caudal puncture was fractioned and stored in anticoagulants solution: Na2EDTA 10%, Na2EDTA 3%, Na heparin 5000 IU and Na heparin 100 IU. Plasmatic levels of calcium presented in the Na2EDTA stored samples were about 80% lower than both heparin groups. Blood samples of P. mesopotamicus stored with Na2EDTA demonstrated increase in the hematocrit and MCV, and decrease in MCHC. The dose-response effect was observed in this study. The results are reinforced by the higher levels of plasmatic protein and hemolysis presented in the Na2EDTA 10% stored blood, confirming the deleterious effect of this anticoagulant treatment on the quality of blood samples. Na2EDTA is not indicated to store P. mesopotamicus blood samples, but sodium heparin at 100 IU is the most recommended anticoagulant, since this treatment presented the lower rate of alterations in the stored blood.
Palavras-Chave: | EDTA; Fish hematology; Hematologia de peixe; Pacus.
Thesagro: | Coagulação Sangüínea; Heparina.
Thesaurus Nal: | Blood coagulation; EDTA (chelating agent); Heparin; Piaractus mesopotamicus.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/143607/1/Na2EDTA-anticoagulant.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (59568-1) - UPE - AP - PP - 636.08905 - P474 -  - DIS