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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Gado de Leite. |
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Data corrente: |
14/12/2020 |
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Data da última atualização: |
14/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FIGUEIRO, M. R.; GARCIA, J. M.; LIMA, M. R. de; COLLADO, M. del; SARAIVA, N. Z. |
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Afiliação: |
MARIVALDO RODRIGUES FIGUEIRO, CPATU; Joaquim Mansano Garcia, UNESP; Marina Ragagnin de Lima, UNESP; Maite del Collado, USP; NAIARA ZOCCAL SARAIVA, CNPGL. |
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Título: |
Is it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use of l-carnitine for in vitro maturation of bubaline oocytes? |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Zygote, v. 28, n. 2, p. 109-115, Apr. 2020. |
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DOI: |
https://doi.org/10.1017/S096719941900073X |
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Idioma: |
Inglês |
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Conteúdo: |
In vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 mM l-carnitine does not increase embryo production. MenosIn vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 ... Mostrar Tudo |
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Palavras-Chave: |
Desenvolvimento embrionário; Soro fetal bovino. |
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Thesagro: |
Búfalo; Lipídio. |
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Thesaurus Nal: |
Buffaloes; Lipid metabolism. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02374naa a2200253 a 4500
001 2128094
005 2020-12-14
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S096719941900073X$2DOI
100 1 $aFIGUEIRO, M. R.
245 $aIs it possible to alter the embryo lipid accumulation with reduction of fetal bovine serum and use of l-carnitine for in vitro maturation of bubaline oocytes?$h[electronic resource]
260 $c2020
520 $aIn vitro embryo production (IVEP) is a procedure that can promote genetic improvement in a short time frame. However, the success rates obtained with this biotechnology in water buffaloes are still inconsistent, and can be associated with the high concentration of lipids in the cytoplasm of oocytes and embryos. The objective of this study was to evaluate the effects of reduced concentration of fetal bovine serum (FBS) and/or use of l-carnitine during in vitro maturation (IVM) on the preimplantation development and lipid accumulation in bubaline embryos. In a first experiment, the lowest concentration of FBS in the IVM medium (0%, 2.5%, 5% or 10%) was determined, and the lowest concentration that maintained good embryo development rates was 5%. In a second experiment, the addition of 5 mM of l-carnitine into the maturation medium was evaluated. The blastocysts produced were submitted to lipid evaluation involving staining followed by observation using optical (Oil Red O) and confocal (BODIPY 493/503) microscopy. No difference was observed between the 5% and 10% FBS groups, which were superior to the 0% and 2.5% groups. Furthermore, the performance of the groups treated with 5% and 10% FBS was better than the groups supplemented with l-carnitine. There was no difference regarding embryo lipid accumulation. The results indicated that it is possible to reduce the FBS concentration to 5% in in vitro maturation medium for production of bubaline embryos, and supplementation with 5 mM l-carnitine does not increase embryo production.
650 $aBuffaloes
650 $aLipid metabolism
650 $aBúfalo
650 $aLipídio
653 $aDesenvolvimento embrionário
653 $aSoro fetal bovino
700 1 $aGARCIA, J. M.
700 1 $aLIMA, M. R. de
700 1 $aCOLLADO, M. del
700 1 $aSARAIVA, N. Z.
773 $tZygote$gv. 28, n. 2, p. 109-115, Apr. 2020.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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Registro Completo
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Biblioteca(s): |
Embrapa Acre. |
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Data corrente: |
02/04/2018 |
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Data da última atualização: |
16/11/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
VASCONCELOS, A. da S.; TOLOTTI, A.; SANTOS, R. S.; AZEVEDO, T. da S.; SUTIL, W. P.; AZEVEDO FILHO, W. S. de. |
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Afiliação: |
Adriana da Silva Vasconcelos; Adriana Tolotti; RODRIGO SOUZA SANTOS, CPAF-Acre; Tatyane da Silva Azevedo; Weidson Plauter Sutil; Wilson Sampaio de Azevedo Filho. |
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Título: |
Novos registros de cigarrinhas (Hemiptera: Cercopidae) associadas às pastagens no Estado do Acre. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA UFAC, 26., 2017, Rio Branco. Anais... Rio Branco: Ufac, 2018. |
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Páginas: |
p. 508. |
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ISBN: |
978-85-8236-078-1 |
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Idioma: |
Português |
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Conteúdo: |
As cigarrinhas-das-pastagens (Hemiptera: Cercopidae) são insetos sugadores de seiva, cujos adultos vivem na parte aérea de gramíneas, e suas ninfas ficam protegidas, na base das plantas, por uma espuma branca característica. Tanto ninfas quanto adultos sugam a seiva da planta, reduzindo os nutrientes disponíveis para o crescimento da parte aérea e raízes. Foi realizado um levantamento das espécies cigarrinhas-das-pastagens na Fazenda Iquiri, município de Senador Guiomard, em pastagem de Brachiaria brizantha cv. Xaraés (MG-5), no período de março de 2015 a maio de 2017. |
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Palavras-Chave: |
Acre; Amazonia Occidental; Amazônia Ocidental; Brachiaria brizantha cv Xaraés; Capim Xaraés; Fazenda Iquiri; First record; Mahanarva mura; Pastizales; Pastos forrajeros; Plagas de plantas; Primeiro registro; Primer registro; Senador Guiomard (AC); Spittlebugs; Western Amazon. |
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Thesagro: |
Cigarrinha das pastagens; Deois flavopicta; Deois incompleta; Gramínea forrageira; Pastagem; Praga de planta; Zulia entreriana. |
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Thesaurus NAL: |
Cercopidae; Forage grasses; Pastures; Plant pests; Urochloa brizantha. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/174837/1/26557.pdf
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Marc: |
LEADER 02143nam a2200529 a 4500
001 2090074
005 2023-11-16
008 2018 bl uuuu u00u1 u #d
020 $a978-85-8236-078-1
100 1 $aVASCONCELOS, A. da S.
245 $aNovos registros de cigarrinhas (Hemiptera$bCercopidae) associadas às pastagens no Estado do Acre.$h[electronic resource]
260 $aIn: SEMINÁRIO DE INICIAÇÃO CIENTÍFICA DA UFAC, 26., 2017, Rio Branco. Anais... Rio Branco: Ufac$c2018
300 $ap. 508.
520 $aAs cigarrinhas-das-pastagens (Hemiptera: Cercopidae) são insetos sugadores de seiva, cujos adultos vivem na parte aérea de gramíneas, e suas ninfas ficam protegidas, na base das plantas, por uma espuma branca característica. Tanto ninfas quanto adultos sugam a seiva da planta, reduzindo os nutrientes disponíveis para o crescimento da parte aérea e raízes. Foi realizado um levantamento das espécies cigarrinhas-das-pastagens na Fazenda Iquiri, município de Senador Guiomard, em pastagem de Brachiaria brizantha cv. Xaraés (MG-5), no período de março de 2015 a maio de 2017.
650 $aCercopidae
650 $aForage grasses
650 $aPastures
650 $aPlant pests
650 $aUrochloa brizantha
650 $aCigarrinha das pastagens
650 $aDeois flavopicta
650 $aDeois incompleta
650 $aGramínea forrageira
650 $aPastagem
650 $aPraga de planta
650 $aZulia entreriana
653 $aAcre
653 $aAmazonia Occidental
653 $aAmazônia Ocidental
653 $aBrachiaria brizantha cv Xaraés
653 $aCapim Xaraés
653 $aFazenda Iquiri
653 $aFirst record
653 $aMahanarva mura
653 $aPastizales
653 $aPastos forrajeros
653 $aPlagas de plantas
653 $aPrimeiro registro
653 $aPrimer registro
653 $aSenador Guiomard (AC)
653 $aSpittlebugs
653 $aWestern Amazon
700 1 $aTOLOTTI, A.
700 1 $aSANTOS, R. S.
700 1 $aAZEVEDO, T. da S.
700 1 $aSUTIL, W. P.
700 1 $aAZEVEDO FILHO, W. S. de
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Embrapa Acre (CPAF-AC) |
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