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| Registros recuperados : 3 | |
| 1. |  | FREITAS NETO, M.; PEREIRA, T. N. S.; GERONIMO, I. G. C.; AZEVEDO, A. O. N.; RAMOS, S. R. R.; PEREIRA, M. G. Coconut genome size determined by flow cytometry: tall versus dwarf types. Genetics and Molecular Research, v. 15, n. 1, february, 2016.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 2. | | LOIOLA, C. M.; AZEVEDO, A. O. N.; DINIZ, L. E. C.; ARAGÃO, W. M.; AZEVEDO, C. D. de O.; SANTOS, P. H. A. D.; RAMOS, H. C. C.; PEREIRA, M. G.; RAMOS, S. R. R. Genetic relationships among tall coconut palm (cocos nucifera l.) accessions of the international coconut genebank for latin america and the caribbean (ICG-LAC), evaluated using microsatellite markers (SSRs). PLOS ONE, march, 2016.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 3. | | AZEVEDO, A. O. N.; AZEVEDO, C. D. de O.; SANTOS, P. H. A. D.; RAMOS, H. C. C.; BOECHAT, M. S. B.; AREDES, F. A. S.; RAMOS, S. R. R.; MIRIZOLA, L. A.; PERERA, L.; ARAGAO, W. M.; PEREIRA, M. G. Selection of legitimate dwarf coconut hybrid seedlings using DNA fingerprinting. Crop Breeding and Applied Biotechnology, v. 18, p. 409-416, 2018.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| Registros recuperados : 3 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
31/03/2016 |
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Data da última atualização: |
26/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FREITAS NETO, M.; PEREIRA, T. N. S.; GERONIMO, I. G. C.; AZEVEDO, A. O. N.; RAMOS, S. R. R.; PEREIRA, M. G. |
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Título: |
Coconut genome size determined by flow cytometry: tall versus dwarf types. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 15, n. 1, february, 2016. |
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DOI: |
http://dx.doi.org/10.4238/gmr.15017470 |
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Idioma: |
Português |
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Conteúdo: |
Coconuts (Cocos nucifera L.) are tropical palm trees that are classified into Tall and Dwarf types based on height, and both types are diploid (2n = 2x = 32 chromosomes). The reproduction mode is autogamous for Dwarf types and allogamous for Tall types. One hypothesis for the origin of the Dwarf coconut suggests that it is a Tall variant that resulted from either mutation or inbreeding, and differences in genome size between the two types would support this hypothesis. In this study, we estimated the genome sizes of 14 coconut accessions (eight Tall and six Dwarf types) using flow cytometry. Nuclei were extracted from leaf discs and stained with propidium iodide, and Pisum sativum (2C = 9.07 pg DNA) was used as an internal standard. Histograms with good resolution and low coefficients of variation (2.5 to 3.2%) were obtained. The 2C DNA content ranged from 5.72 to 5.48 pg for Tall accessions and from 5.58 to 5.52 pg for Dwarf accessions. The mean genome sizes for Tall and Dwarf specimens were 5.59 and 5.55 pg, respectively. Among all accessions, Rennel Island Tall had the highest mean DNA content (5.72 pg), whereas West African Tall had the lowest (5.48 pg). The mean coconut genome size (2C = 5.57 pg, 2M. Freitas Neto et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 15 (1): gmr.15017470 corresponding to 2723.73 Mbp/haploid set) was classified as small. Only small differences in genome size existed among the coconut accessions, suggesting that the Dwarf type did not evolve from the Tall type. MenosCoconuts (Cocos nucifera L.) are tropical palm trees that are classified into Tall and Dwarf types based on height, and both types are diploid (2n = 2x = 32 chromosomes). The reproduction mode is autogamous for Dwarf types and allogamous for Tall types. One hypothesis for the origin of the Dwarf coconut suggests that it is a Tall variant that resulted from either mutation or inbreeding, and differences in genome size between the two types would support this hypothesis. In this study, we estimated the genome sizes of 14 coconut accessions (eight Tall and six Dwarf types) using flow cytometry. Nuclei were extracted from leaf discs and stained with propidium iodide, and Pisum sativum (2C = 9.07 pg DNA) was used as an internal standard. Histograms with good resolution and low coefficients of variation (2.5 to 3.2%) were obtained. The 2C DNA content ranged from 5.72 to 5.48 pg for Tall accessions and from 5.58 to 5.52 pg for Dwarf accessions. The mean genome sizes for Tall and Dwarf specimens were 5.59 and 5.55 pg, respectively. Among all accessions, Rennel Island Tall had the highest mean DNA content (5.72 pg), whereas West African Tall had the lowest (5.48 pg). The mean coconut genome size (2C = 5.57 pg, 2M. Freitas Neto et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 15 (1): gmr.15017470 corresponding to 2723.73 Mbp/haploid set) was classified as small. Only small differences in genome size existed among the coconut accessions, suggesting that the Dwarf ty... Mostrar Tudo |
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Palavras-Chave: |
C-value; Coconut accessions; Cocos nucifera L; DNA content; Palm tree. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141883/1/gmr7470.pdf
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Marc: |
LEADER 02294naa a2200253 a 4500
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008 2016 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/gmr.15017470$2DOI
100 1 $aFREITAS NETO, M.
245 $aCoconut genome size determined by flow cytometry$btall versus dwarf types.$h[electronic resource]
260 $c2016
520 $aCoconuts (Cocos nucifera L.) are tropical palm trees that are classified into Tall and Dwarf types based on height, and both types are diploid (2n = 2x = 32 chromosomes). The reproduction mode is autogamous for Dwarf types and allogamous for Tall types. One hypothesis for the origin of the Dwarf coconut suggests that it is a Tall variant that resulted from either mutation or inbreeding, and differences in genome size between the two types would support this hypothesis. In this study, we estimated the genome sizes of 14 coconut accessions (eight Tall and six Dwarf types) using flow cytometry. Nuclei were extracted from leaf discs and stained with propidium iodide, and Pisum sativum (2C = 9.07 pg DNA) was used as an internal standard. Histograms with good resolution and low coefficients of variation (2.5 to 3.2%) were obtained. The 2C DNA content ranged from 5.72 to 5.48 pg for Tall accessions and from 5.58 to 5.52 pg for Dwarf accessions. The mean genome sizes for Tall and Dwarf specimens were 5.59 and 5.55 pg, respectively. Among all accessions, Rennel Island Tall had the highest mean DNA content (5.72 pg), whereas West African Tall had the lowest (5.48 pg). The mean coconut genome size (2C = 5.57 pg, 2M. Freitas Neto et al.©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 15 (1): gmr.15017470 corresponding to 2723.73 Mbp/haploid set) was classified as small. Only small differences in genome size existed among the coconut accessions, suggesting that the Dwarf type did not evolve from the Tall type.
653 $aC-value
653 $aCoconut accessions
653 $aCocos nucifera L
653 $aDNA content
653 $aPalm tree
700 1 $aPEREIRA, T. N. S.
700 1 $aGERONIMO, I. G. C.
700 1 $aAZEVEDO, A. O. N.
700 1 $aRAMOS, S. R. R.
700 1 $aPEREIRA, M. G.
773 $tGenetics and Molecular Research$gv. 15, n. 1, february, 2016.
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