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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
06/02/2017 |
Data da última atualização: |
06/12/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ARENA, G. D.; RAMOS-GONZÁLEZ, P. L.; NUNES, M. A A.; RIBEIRO-ALVES, M.; RIBEIRO-ALVES, M.; CAMARGO, L. E. A.; KITAJIMA, E. T W.; MACHADO, M. A.; ASTUA, J. de F. |
Afiliação: |
GABRIELLA D. ARENA, APTA; PEDRO L. RAMOS-GONZÁLEZ, APTA; MARIA A. NUNES, APTA; MARCELO RIBEIRO-ALVES; MARCELO RIBEIRO-ALVES, FIOCRUZ; LUIS E. A. CAMARGO, ESALQ; ELLIOT W. KITAJIMA, ESALQ; MARCOS A. MACHADO, APTA; JULIANA DE FREITAS ASTUA, CNPMF. |
Título: |
Citrus leprosis virus C Infection Results in Hypersensitive-Like Response, Suppression of the JA/ET Plant Defense Pathway and Promotion of the Colonization of Its Mite Vector. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Frontiers in Plant Science November 2016. |
ISSN: |
1664-462X |
DOI: |
10.3389/fpls.2016.01757 |
Idioma: |
Inglês |
Conteúdo: |
Leprosis is a serious disease of citrus caused by Citrus leprosis virus C (CiLV-C, genus Cilevirus) whose transmission is mediated by false spider mites of the genus Brevipalpus. CiLV-C infection does not systemically spread in any of its known host plants, thus remaining restricted to local lesions around the feeding sites of viruliferous mites. To get insight into this unusual pathosystem, we evaluated the expression profiles of genes involved in defense mechanisms of Arabidopsis thaliana and Citrus sinensis upon infestation with non-viruliferous and viruliferous mites by using reverse-transcription qPCR. These results were analyzed together with the production of reactive oxygen species (ROS) and the appearance of dead cells as assessed by histochemical assays. After interaction with non-viruliferous mites, plants locally accumulated ROS and triggered the salicylic acid (SA) and jasmonate/ethylene (JA/ET) pathways. ERF branch of the JA/ET pathways was highly activated. In contrast, JA pathway genes were markedly suppressed upon the CiLV-C infection mediated by viruliferous mites. Viral infection also intensified the ROS burst and cell death, and enhanced the expression of genes involved in the RNA silencing mechanism and SA pathway. After 13 days of infestation of two sets of Arabidopsis plants with non-viruliferous and viruliferous mites, the number of mites in the CiLV-C infected Arabidopsis plants was significantly higher than in those infested with the non-viruliferous ones. Oviposition of the viruliferous mites occurred preferentially in the CiLV-C infected leaves. Based on these results, we postulated the first model of plant/Brevipalpus mite/cilevirus interaction in which cells surrounding the feeding sites of viruliferous mites typify the outcome of a hypersensitive-like response, whereas viral infection induces changes in the behavior of its vector. MenosLeprosis is a serious disease of citrus caused by Citrus leprosis virus C (CiLV-C, genus Cilevirus) whose transmission is mediated by false spider mites of the genus Brevipalpus. CiLV-C infection does not systemically spread in any of its known host plants, thus remaining restricted to local lesions around the feeding sites of viruliferous mites. To get insight into this unusual pathosystem, we evaluated the expression profiles of genes involved in defense mechanisms of Arabidopsis thaliana and Citrus sinensis upon infestation with non-viruliferous and viruliferous mites by using reverse-transcription qPCR. These results were analyzed together with the production of reactive oxygen species (ROS) and the appearance of dead cells as assessed by histochemical assays. After interaction with non-viruliferous mites, plants locally accumulated ROS and triggered the salicylic acid (SA) and jasmonate/ethylene (JA/ET) pathways. ERF branch of the JA/ET pathways was highly activated. In contrast, JA pathway genes were markedly suppressed upon the CiLV-C infection mediated by viruliferous mites. Viral infection also intensified the ROS burst and cell death, and enhanced the expression of genes involved in the RNA silencing mechanism and SA pathway. After 13 days of infestation of two sets of Arabidopsis plants with non-viruliferous and viruliferous mites, the number of mites in the CiLV-C infected Arabidopsis plants was significantly higher than in those infested with the non-viruliferou... Mostrar Tudo |
Thesagro: |
Doença de planta. |
Categoria do assunto: |
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Marc: |
LEADER 02729naa a2200253 a 4500 001 2062769 005 2017-12-06 008 2016 bl uuuu u00u1 u #d 022 $a1664-462X 024 7 $a10.3389/fpls.2016.01757$2DOI 100 1 $aARENA, G. D. 245 $aCitrus leprosis virus C Infection Results in Hypersensitive-Like Response, Suppression of the JA/ET Plant Defense Pathway and Promotion of the Colonization of Its Mite Vector.$h[electronic resource] 260 $c2016 520 $aLeprosis is a serious disease of citrus caused by Citrus leprosis virus C (CiLV-C, genus Cilevirus) whose transmission is mediated by false spider mites of the genus Brevipalpus. CiLV-C infection does not systemically spread in any of its known host plants, thus remaining restricted to local lesions around the feeding sites of viruliferous mites. To get insight into this unusual pathosystem, we evaluated the expression profiles of genes involved in defense mechanisms of Arabidopsis thaliana and Citrus sinensis upon infestation with non-viruliferous and viruliferous mites by using reverse-transcription qPCR. These results were analyzed together with the production of reactive oxygen species (ROS) and the appearance of dead cells as assessed by histochemical assays. After interaction with non-viruliferous mites, plants locally accumulated ROS and triggered the salicylic acid (SA) and jasmonate/ethylene (JA/ET) pathways. ERF branch of the JA/ET pathways was highly activated. In contrast, JA pathway genes were markedly suppressed upon the CiLV-C infection mediated by viruliferous mites. Viral infection also intensified the ROS burst and cell death, and enhanced the expression of genes involved in the RNA silencing mechanism and SA pathway. After 13 days of infestation of two sets of Arabidopsis plants with non-viruliferous and viruliferous mites, the number of mites in the CiLV-C infected Arabidopsis plants was significantly higher than in those infested with the non-viruliferous ones. Oviposition of the viruliferous mites occurred preferentially in the CiLV-C infected leaves. Based on these results, we postulated the first model of plant/Brevipalpus mite/cilevirus interaction in which cells surrounding the feeding sites of viruliferous mites typify the outcome of a hypersensitive-like response, whereas viral infection induces changes in the behavior of its vector. 650 $aDoença de planta 700 1 $aRAMOS-GONZÁLEZ, P. L. 700 1 $aNUNES, M. A A. 700 1 $aRIBEIRO-ALVES, M. 700 1 $aRIBEIRO-ALVES, M. 700 1 $aCAMARGO, L. E. A. 700 1 $aKITAJIMA, E. T W. 700 1 $aMACHADO, M. A. 700 1 $aASTUA, J. de F. 773 $tFrontiers in Plant Science November 2016.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
22/02/2013 |
Data da última atualização: |
15/03/2013 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
NOVELLI, V. M.; NUNES, M. A.; LATADO, R. R.; FERREIRA, D. N. M.; MENDONÇA, R. S.; BERGAMINI, M. P.; BASTIANEL, M.; ASTUA, J. de F. |
Afiliação: |
VALDENICE MOREIRA NOVELLI, IAC; MARIA ANDREIA NUNES, IAC; RODRIGO ROCHA LATADO, IAC; DENISE NAVIA MAGALHAES FERREIRA, CENARGEN; RENATA SANTOS MENDONÇA, CENARGEN; MARCELA PAGOTI BERGAMINI, IAC; MARINÊS BASTIANEL, IAC; JULIANA DE FREITAS ASTUA, CNPMF. |
Título: |
The genome size of Brevipalpus phoenicis estimated by flow cytometry: preliminary results. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
In: SYMPOSIUM OF THE EUROPEAN ASSOCIATION OF ACAROLOGISTS, 7., 2012, Viena. Abstracts... Vienna, Austria, 2012. |
Idioma: |
Inglês |
Notas: |
Anais Web. |
Conteúdo: |
Brevipalpus phoenicis (Geijskes) (Acari: Tenuipalpidae) is one of the most important species within the genus; it is highly polyphagous, has worldwide distribution and is also a vector of severa I plant viruses, including Citrus leprosis virus C (CiLV-C), one of the main diseases of citrus in Brazil. |
Thesagro: |
Brevipalpus Phoenicis. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01015nam a2200217 a 4500 001 1950629 005 2013-03-15 008 2012 bl uuuu u00u1 u #d 100 1 $aNOVELLI, V. M. 245 $aThe genome size of Brevipalpus phoenicis estimated by flow cytometry$bpreliminary results.$h[electronic resource] 260 $aIn: SYMPOSIUM OF THE EUROPEAN ASSOCIATION OF ACAROLOGISTS, 7., 2012, Viena. Abstracts... Vienna, Austria$c2012 500 $aAnais Web. 520 $aBrevipalpus phoenicis (Geijskes) (Acari: Tenuipalpidae) is one of the most important species within the genus; it is highly polyphagous, has worldwide distribution and is also a vector of severa I plant viruses, including Citrus leprosis virus C (CiLV-C), one of the main diseases of citrus in Brazil. 650 $aBrevipalpus Phoenicis 700 1 $aNUNES, M. A. 700 1 $aLATADO, R. R. 700 1 $aFERREIRA, D. N. M. 700 1 $aMENDONÇA, R. S. 700 1 $aBERGAMINI, M. P. 700 1 $aBASTIANEL, M. 700 1 $aASTUA, J. de F.
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