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Registro Completo |
Biblioteca(s): |
Embrapa Meio-Norte. |
Data corrente: |
01/12/1998 |
Data da última atualização: |
24/07/2009 |
Autoria: |
SOUZA, V. A. B. de; ARAUJO, E. E. A.; VASCONCELOS, L. F. L.; LIMA, P. S. da C.; ALCOFORADO FILHO, F. G. |
Afiliação: |
EMBRAPA-CPAMN. |
Título: |
Coleta e caracterizacao fisico-quimica de frutos de germoplasma de bacuri (Platonia insignis Mart.) da regiao Meio-Norte do Brasil. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE GENETICA, 44., 1998, Aguas de Lindoia. Programa e resumos... Aguas de Lindoia: SBG, 1998. Genetics and Molecular Biology, v.21, n.3, p.217. Supplement. Resumo n.32. |
Idioma: |
Português |
Palavras-Chave: |
Brasil; Coleta; Regiao Meio-Norte. |
Thesagro: |
Bacuri; Germoplasma; Platonia Insignis. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00843naa a2200229 a 4500 001 1054497 005 2009-07-24 008 1998 bl uuuu u00u1 u #d 100 1 $aSOUZA, V. A. B. de 245 $aColeta e caracterizacao fisico-quimica de frutos de germoplasma de bacuri (Platonia insignis Mart.) da regiao Meio-Norte do Brasil. 260 $c1998 650 $aBacuri 650 $aGermoplasma 650 $aPlatonia Insignis 653 $aBrasil 653 $aColeta 653 $aRegiao Meio-Norte 700 1 $aARAUJO, E. E. A. 700 1 $aVASCONCELOS, L. F. L. 700 1 $aLIMA, P. S. da C. 700 1 $aALCOFORADO FILHO, F. G. 773 $tIn: CONGRESSO NACIONAL DE GENETICA, 44., 1998, Aguas de Lindoia. Programa e resumos... Aguas de Lindoia: SBG, 1998. Genetics and Molecular Biology$gv.21, n.3, p.217. Supplement. Resumo n.32.
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
01/06/2023 |
Data da última atualização: |
01/06/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
DORTA, S. DE O.; ATTÍLIO, L. B.; ZANARDI, O. Z.; LOPES, J. R. S.; MACHADO, M. A.; ASTUA, J. de F. |
Afiliação: |
SÍLVIA DE OLIVEIRA DORTA, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; LÍSIA BORGES ATTÍLIO, INSTITUTO AGRONÔMICO DE CAMPINAS; ODIMAR ZANUZO ZANARDI, INSTITUTO FEDERAL DE SANTA CATARINA; JOÃO ROBERTO SPOTTI LOPES, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; MARCOS ANTONIO MACHADO, INSTITUTO AGRONÔMICO DE CAMPINAS; JULIANA DE FREITAS ASTUA, CNPMF. |
Título: |
Genetic transformation of 'Hamlin' and 'Valencia' sweet orange plants expressing the cry11A gene of Bacillus thuringiensis as an additional tool for the management of Diaphorina citri (Hemiptera: Liviidae). |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Journal of Biotechnolog, v.68, May, p.60-70, 2023. |
ISSN: |
0168-1656 |
DOI: |
https://doi.org/10.1016/j.jbiotec.2023.04.007 |
Idioma: |
Inglês |
Conteúdo: |
The Asian citrus psyllid (ACP) Diaphorina citri Kuwayama (Hemiptera: Liviidae) is the vector of Candidatus Liberibacter spp., the bacteria associated with huanglongbing (HLB), the most devastating disease of citrus worldwide. HLB management has heavily relied on insecticide applications to control the ACP, although there are efforts towards more sustainable alternatives. In previous work, our group assessed the potential bioactivity of different strains of Bacillus thuringiensis (Eubacteriales: Bacillaceae) (Bt) containing cry/cyt genes as feasible tools to control ACP nymphs. Here, we report an attempt to use the cry11A gene from Bt to produce transgenic sweet orange plants using two promoters. For the genetic transformation, ?Hamlin? and ?Valencia? sweet orange seedlings were used as sources of explants. Transgenic plants were detected by polymerase chain reaction (PCR) with specific primers, and the transgene copy number was confirmed by Southern blot analyses. Transcript expression levels were determined by qPCR. Mortality assays of D. citri nymphs were carried out in a greenhouse, and the effect of the events tested ranged from 22% to 43% at the end of the five-day exposure period. To our knowledge, this is the first manuscript reporting the production of citrus plants expressing the Bt cry11A gene for the management of D. citri nymphs. |
Thesagro: |
Laranja; Melhoramento Vegetal. |
Thesaurus NAL: |
Oranges. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02204naa a2200241 a 4500 001 2154159 005 2023-06-01 008 2023 bl uuuu u00u1 u #d 022 $a0168-1656 024 7 $ahttps://doi.org/10.1016/j.jbiotec.2023.04.007$2DOI 100 1 $aDORTA, S. DE O. 245 $aGenetic transformation of 'Hamlin' and 'Valencia' sweet orange plants expressing the cry11A gene of Bacillus thuringiensis as an additional tool for the management of Diaphorina citri (Hemiptera$bLiviidae).$h[electronic resource] 260 $c2023 520 $aThe Asian citrus psyllid (ACP) Diaphorina citri Kuwayama (Hemiptera: Liviidae) is the vector of Candidatus Liberibacter spp., the bacteria associated with huanglongbing (HLB), the most devastating disease of citrus worldwide. HLB management has heavily relied on insecticide applications to control the ACP, although there are efforts towards more sustainable alternatives. In previous work, our group assessed the potential bioactivity of different strains of Bacillus thuringiensis (Eubacteriales: Bacillaceae) (Bt) containing cry/cyt genes as feasible tools to control ACP nymphs. Here, we report an attempt to use the cry11A gene from Bt to produce transgenic sweet orange plants using two promoters. For the genetic transformation, ?Hamlin? and ?Valencia? sweet orange seedlings were used as sources of explants. Transgenic plants were detected by polymerase chain reaction (PCR) with specific primers, and the transgene copy number was confirmed by Southern blot analyses. Transcript expression levels were determined by qPCR. Mortality assays of D. citri nymphs were carried out in a greenhouse, and the effect of the events tested ranged from 22% to 43% at the end of the five-day exposure period. To our knowledge, this is the first manuscript reporting the production of citrus plants expressing the Bt cry11A gene for the management of D. citri nymphs. 650 $aOranges 650 $aLaranja 650 $aMelhoramento Vegetal 700 1 $aATTÍLIO, L. B. 700 1 $aZANARDI, O. Z. 700 1 $aLOPES, J. R. S. 700 1 $aMACHADO, M. A. 700 1 $aASTUA, J. de F. 773 $tJournal of Biotechnolog$gv.68, May, p.60-70, 2023.
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