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| Registros recuperados : 180 | |
| 81. |  | LEASTRO, M. O.; VILLAR-ÁLVAREZ, D.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. Spontaneous mutation in the movement protein of Citrus Leprosis Virus C2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage. Viruses , v.13, n.12, 2021.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 83. | | FERREIRA, D. N. M.; NOVELLI, V. M.; POLEZ, V. L. P.; CAMPOS, E.; NUNES, M. A.; MENDONÇA, R. S.; PIMENTA, L.; ASTUA, J. de F. The flat mite Brevipalpus phoenicis (Acari: Tenuipalpidae) genome project: update and perspectives. In: SYMPOSIUM OF THE EUROPEAN ASSOCIATION OF ACAROLOGISTS, 7., 2012, Viena. Program, abstracts, participantes... Viena: University of Natural Resources and life Sicences, 2012. p. 55 1 CD-ROM Anais Web.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 84. | | FERREIRA, D. N. M.; NOVELLI, V. M.; POLEZ, V. L. P.; CAMPOS, E.; NUNES, M. A.; MENDONÇA, R. S.; PIMENTA, L.; ASTUA, J. de F. The flat mite Brevipalpus phoenicis (Acari: Tenuipalpidae) genome project: update and perspectives. In: SYMPOSIUM OF THE EUROPEAN ASSOCIATION OF ACAROLOGISTS, 7., 2012, Viena. Abstracts... Vienna, Austria, 2012. Anais Web.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 85. | | SAUVÊTRE, P.; VENIANT, E.; CROQ, G.; TASSI, A. D.; KITAJIMA, E. W.; CHABI-JESUS, C.; RAMOS-GONZÁLEZ, P. L.; ASTUA, J. de F.; FERREIRA, D. N. M. First report of orchid fleck virus in the orchid collection of Jardin du Luxembourg, Paris, France. Plant Disease, v. 102, n. 12, p. 2670, 2018. Na publicação: J. Freitas-Astúa, D. Navia.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 86. | | COSTA-RODRIGUES, M. da; PEREIRA, L. R.; RAMOS-GONZÁLEZ, P. L.; TASSI, D.; LEÃO, I. G.; HARAKAVA, R.; KITAJIMA, E. W.; ASTUA, J. de F. First report of Passion fruit green spot virusinfecting hibiscus plants. New Disease Report, v.45, n.2, e12080, 2022.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 88. | | NUNES, M. A.; MINEIRO, L. de C.; ROGERIO, L. A.; FERREIRA, L. M.; TASSI, A.; NOVELLI, V. M.; KITAJIMA, E. W.; ASTUA, J. de F. First Report of Brevipalpus papayensis as Vector of Coffee ringspot virus and Citrus leprosis virus C Plant Disease, v.102, n. 5, p.1046, May 2018. Disease Note.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 89. | | CHABI-JESUS, C.; RAMOS-GONZÁLEZ, P. L.; TASSI, A. D.; BARGUIL, B. M.; BESERRA JUNIOR, J. E. A.; HARAKAVA, R.; KITAJIMA, E. W.; ASTUA, J. de F. First Report of Citrus Chlorotic Spot Virus Infecting the Succulent Plant Agave desmettiana. Plant Disease, v.103, n. 6, June 2019.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 90. | | GARITA, L. C.; TASSI, A. D.; CALEGARIO, R. F.; ASTUA, J. de F.; SALAROLI, R. B.; ROMÃO, G. O.; KITAJIMA, E. W. Experimental host range of Citrus leprosis virus C (CiLV-C). Tropical Plant Pathology, v. 39, n.1, p.043-055, 2014.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 91. | | NUNES, M. A; NOVELLI, V. M.; CUNHA, B. A. da; SOARES, A. J.; MINEIRO, J. L. C. de; ASTUA, J. de F.; BASTIANEL, M. Survey of the citrus leprosis vector (Brevipalpus yothersi) and phytoseiids in spontaneous plants of an organic citrus orchard. Experimental and Applied Acarology, v. 82, p.199?209, 2020.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 92. | | NUNES, M. A.; LAMEIRO, P.; CUNHA, B. A. da; SOARES, A. J.; MINEIR0, J. L. DE C.; ASTUA, J. de F.; NOVELLI, V. M.; BASTIANEL, M. Survey of phytoseiids and mite-transmitted viruses in weeds from an organic sweet orange orchard in Brazil. In: SYMPOSIUM OF THE EUROPEAN ASSOCIATION OF ACAROLOGISTS, 7., 2012, Viena. Abstracts... Vienna, Austria, 2012. Anais Web.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 93. | | CHABI-JESUS, C.; RAMOS-GONZÁLEZ, P. L.; TASSI, A. D.; GUERRA-PERAZA, O.; KITAJIMA, E. W.; HARAKAVA, R.; BESERRA, JR. J. E. A.; ASTUA, J. de F. Identification and characterization of citrus chlorotic spot virus, a new dichorhavirus associated with citrus leprosis-like symptoms. Plant Disease, v.102, p. 1588-1598, 2018.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 95. | | BARBOSA, F. F. L.; SILVA, S. X. B.; ANDRADE, E. C. de; ASTUA, J. de F.; SILVA, T. S. M. da; SOARES, A. C. F.; ALMEIDA, D. O. Influência de variáveis climáticas na dinâmica de infestação de Brevipalpus phoenicis (Geijskes, 1939) (Acari: Tenuipalpidae) em pomares cítricos no recôncavo baiano. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 24., 2012, Curitiba. SEB - 40 anos de avanços da Ciência Entomológica Brasileira. Curitiba: SEB, 2012. Anais Web| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 96. | | ARENA, G. D.; RAMOS-GONZÁLEZ, P. L.; ROGERIO, L. A.; RIBEIRO-ALVES, M.; CASTEEL, C. L.; ASTUA, J. de F.; MACHADO, M. A. Making a better home: modulation of plant defensive response by brevipalpus mites. Frontiers in Plant Science, v.9, August, 2018.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 97. | | DORTA, S. de O.; ATTÍLIO, L. B.; LOPES, J. R. S.; PONTES, R. G. M. S. de; MACHADO, M. A.; ASTUA, J. de F. Atividade inseticida de Bacillus thuringiensis contra Diaphorina citri e produção de plantas transgênicas de citros Bt. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 15., 2017, Ribeirão Preto. Os novos desafios do controle. São Paulo: FCAV/Unesp: ESALQ, 2017.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 98. | | ARENA, G. D.; RAMOS-GONZÁLEZ, P. L.; NUNES, M. A.; JESUS, C. C.; CALEGARIO, R. F.; KITAJIMA, E. W.; NOVELLI, V. M.; ASTUA, J. de F. Arabidopsis thaliana as a model host for Brevipalpus mite-transmitted viruses. Scientia Agricola, v.74, n.1, p.1-10X, 2017| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 99. | | ASTUA, J. de F.; KITAJIMA, E. W.; KUBO, K. S.; BASTIANEL, M.; CRISTOFANI-VALY, M.; LOCALI-FABRIS, E.; NOVELLI, V. M.; MACHADO, M. A. The atypical Brevipalpus-transmitted viruses and their interactions with host plants and mite vectors. In: EMBO WORLD LECTURE COURSE VIRUS-HOST: PARTNERS IN PATHOGENICITY, 2010, San Jose. [Abstracts...].San Jose: EMBO, 2010. p. 40. Organizado por Cecilia Ramirez.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 100. | | SÁNCHEZ-VELÁZQUEZ, E. J.; SANTILLÁN-GALICIA, M. T.; NOVELLI, V. M.; NUNES, M. A.; MORA-AGUILERA, G.; VALDEZ-CARRASCO, J. M.; OTERO-COLINA, G.; ASTUA, J. de F. Diversity and genetic variation among brevipalpus populations from Brazil and Mexico. PLOS ONE, v. 10, n. 7, p.1-16, 2015.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 180 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
17/11/2021 |
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Data da última atualização: |
17/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
BEZERRA, Y. C. dos A.; MARQUES, J. P. R.; STIPP, L. C. L.; ATTÍLIO, L. B.; ASTUA, J. de F.; MOURÃO FILHO, F. de A. A. |
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Afiliação: |
YANE CAROLINE DOS ANJOS BEZERRA, USP; JOÃO PAULO RODRIGUES MARQUES, USP; LILIANE CRISTINA LIBORIO STIPP, USP; LÍSIA BORGES ATTÍLIO, USP; JULIANA DE FREITAS ASTUA, CNPMF; FRANCISCO DE ASSIS ALVES MOURÃO FILHO, USP. |
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Título: |
How to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Revista Brasileira de Fruticultura, Jaboticabal, v. 43, n. 4,e-005, 2021. |
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ISSN: |
1806-9967 |
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Idioma: |
Português |
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Conteúdo: |
New approaches for developing disease-resistant genetically modified organisms have included specific targets for gene expression to enhance the chances for pathogen control. Gene expression driven by phloem-derived Citrus sinensis gene promoters could be evaluated and compared with the expression induced by a strong constitutive promoter in the same tissue, leading to the production of transgenic sweet oranges potentially more resistant to diseases caused by phloem-limited bacteria. ?Carrizo? citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck] was transformed, via Agrobacterium tumefaciens, with the binary vector pCAMBIA2301 bearing the uidA gene (?-glucuronidase) driven by the CaMV35S constitutive promoter (CaMV35S::uidA) or by the CsPP2.B1 (CsPP2.B1::uidA) or by the CsVTE2 (CsVTE2::uidA) citrus promoters. In vitro regenerated shoots were grafted onto ?Rangpur? lime (C. limonia Osbeck). The genetic transformation was confirmed by Southern blot analyses. uidA gene expression was evaluated by RT-qPCR, and gene histolocalization controlled by these three promoters was accessed by X-GLUC treated stem sections. uidA gene expression exhibited by tissue-specific promoters was overall lower than from the constitutive promoter CaMV35; however, constructs driven by tissuespecific promoters may lead to expression in restricted tissues. CsPP2.B1 and CsVTE2 promoters can be considered adequate for the utilization in gene constructs aiming disease resistance. |
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Thesagro: |
Fruta Cítrica. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227820/1/How-to-drive-phloem-gene-expression.pdf
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Marc: |
LEADER 02150naa a2200205 a 4500
001 2136203
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022 $a1806-9967
100 1 $aBEZERRA, Y. C. dos A.
245 $aHow to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters.$h[electronic resource]
260 $c2021
520 $aNew approaches for developing disease-resistant genetically modified organisms have included specific targets for gene expression to enhance the chances for pathogen control. Gene expression driven by phloem-derived Citrus sinensis gene promoters could be evaluated and compared with the expression induced by a strong constitutive promoter in the same tissue, leading to the production of transgenic sweet oranges potentially more resistant to diseases caused by phloem-limited bacteria. ?Carrizo? citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck] was transformed, via Agrobacterium tumefaciens, with the binary vector pCAMBIA2301 bearing the uidA gene (?-glucuronidase) driven by the CaMV35S constitutive promoter (CaMV35S::uidA) or by the CsPP2.B1 (CsPP2.B1::uidA) or by the CsVTE2 (CsVTE2::uidA) citrus promoters. In vitro regenerated shoots were grafted onto ?Rangpur? lime (C. limonia Osbeck). The genetic transformation was confirmed by Southern blot analyses. uidA gene expression was evaluated by RT-qPCR, and gene histolocalization controlled by these three promoters was accessed by X-GLUC treated stem sections. uidA gene expression exhibited by tissue-specific promoters was overall lower than from the constitutive promoter CaMV35; however, constructs driven by tissuespecific promoters may lead to expression in restricted tissues. CsPP2.B1 and CsVTE2 promoters can be considered adequate for the utilization in gene constructs aiming disease resistance.
650 $aFruta Cítrica
700 1 $aMARQUES, J. P. R.
700 1 $aSTIPP, L. C. L.
700 1 $aATTÍLIO, L. B.
700 1 $aASTUA, J. de F.
700 1 $aMOURÃO FILHO, F. de A. A.
773 $tRevista Brasileira de Fruticultura, Jaboticabal$gv. 43, n. 4,e-005, 2021.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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