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Registros recuperados : 181 | |
21. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | LEASTRO, M. O.; KITAJIMA, E. W.; SILVA, M. S.; RESENDE, R. O.; ASTUA, J. de F. Dissecting the subcellular localization, intracellular trafficking, interactions, membrane association, and topology of Citrus Leprosis Virus. C proteins. Frontiers in Plant Science, v. 19, 2018. Biblioteca(s): Embrapa Mandioca e Fruticultura; Embrapa Recursos Genéticos e Biotecnologia. |
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37. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SAITO, M.; ASTUA, J. de F.; RAMOS-GONZALEZ, P.; NOVELLI, V. M.; KITAJIMA, E. W. Detection of Passion fruit green spot virus (PFGSV), a Brevipalpus transmitted virus in the mite vector B. yothersi In: SYMPOSIUM OF THE EUROPEAN ASSOCIATION OF ACAROLOGISTS, 8., 2016, València. [Abstracts...]. Valencia: Universitat Politècnica de Valencia, 2016. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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40. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | PEREIRA, J. A.; NUNES, M. A.; BRETON, M. C.; MACHADO, M. A.; ASTUA, J. de F. Genetic variability of P29, the putative coat protein gene of Citrus Leprosis Virus C. In: Conference of the International Organization of Citrus Virologists 19. Mpumalanga, South Africa, 2013. Program & Abstracts... Mpumalanga, South, 2013. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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Registros recuperados : 181 | |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
08/02/2021 |
Data da última atualização: |
09/02/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LEASTRO, M. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; NAVARRO, J. S. |
Afiliação: |
MIKHAIL OLIVEIRA LEASTRO, Instituto Biológico, São Paulo; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT WATANABE KITAJIMA, ESALQ; VICENTE PALLÁS, Universidad Politécnica de Valencia; JESÚSA. SÁNCHEZ NAVARRO, Universidad Politécnica de Valencia. |
Título: |
Unravelling the involvement of cilevirus p32 protein in the viral transport. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Scientifc Reports, n.11, v.2943, 2021. |
Idioma: |
Inglês |
Conteúdo: |
Citrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model for the role of the p32 in the intra- and intercellular viral spread. MenosCitrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model... Mostrar Tudo |
Thesagro: |
Doença de Planta; Fruta Cítrica. |
Thesaurus NAL: |
Citrus; Plant diseases and disorders. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02172naa a2200217 a 4500 001 2129841 005 2021-02-09 008 2021 bl uuuu u00u1 u #d 100 1 $aLEASTRO, M. O. 245 $aUnravelling the involvement of cilevirus p32 protein in the viral transport.$h[electronic resource] 260 $c2021 520 $aCitrus leprosis (CL) is a severe disease that affects citrus orchards mainly in Latin America. It is caused by Brevipalpus-transmitted viruses from genera Cilevirus and Dichorhavirus. Currently, no reports have explored the movement machinery for the cilevirus. Here, we have performed a detailed functional study of the p32 movement protein (MP) of two cileviruses. Citrus leprosis-associated viruses are not able to move systemically in neither their natural nor experimental host plants. However, here we show that cilevirus MPs are able to allow the cell-to-cell and long-distance transport of movement-defective alfalfa mosaic virus (AMV). Several features related with the viral transport were explored, including: (i) the ability of cilevirus MPs to facilitate virus movement on a nucleocapsid assembly independent-manner; (ii) the generation of tubular structures from transient expression in protoplast; (iii) the capability of the N- and C- terminus of MP to interact with the cognate capsid protein (p29) and; (iv) the role of the C-terminus of p32 in the cell-to-cell and long-distance transport, tubule formation and the MP-plasmodesmata co-localization. The MP was able to direct the p29 to the plasmodesmata, whereby the C-terminus of MP is independently responsible to recruit the p29 to the cell periphery. Furthermore, we report that MP possess the capacity to enter the nucleolus and to bind to a major nucleolar protein, the fibrillarin. Based on our findings, we provide a model for the role of the p32 in the intra- and intercellular viral spread. 650 $aCitrus 650 $aPlant diseases and disorders 650 $aDoença de Planta 650 $aFruta Cítrica 700 1 $aASTUA, J. de F. 700 1 $aKITAJIMA, E. W. 700 1 $aPALLÁS, V. 700 1 $aNAVARRO, J. S. 773 $tScientifc Reports$gn.11
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