Data/hora: 20/06/2024 04:48:42

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 17/11/2021
Data da última atualização: | 17/11/2021
Autoria: | BEZERRA, Y. C. dos A.; MARQUES, J. P. R.; STIPP, L. C. L.; ATTÍLIO, L. B.; ASTUA, J. de F.; MOURÃO FILHO, F. de A. A.
Afiliação: | YANE CAROLINE DOS ANJOS BEZERRA, USP; JOÃO PAULO RODRIGUES MARQUES, USP; LILIANE CRISTINA LIBORIO STIPP, USP; LÍSIA BORGES ATTÍLIO, USP; JULIANA DE FREITAS ASTUA, CNPMF; FRANCISCO DE ASSIS ALVES MOURÃO FILHO, USP.
Título: | How to drive phloem gene expression? A case study with preferentially expressed citrus gene promoters.
Ano de publicação: | 2021
Fonte/Imprenta: | Revista Brasileira de Fruticultura, Jaboticabal, v. 43, n. 4,e-005, 2021.
ISSN: | 1806-9967
Idioma: | Português
Conteúdo: | New approaches for developing disease-resistant genetically modified organisms have included specific targets for gene expression to enhance the chances for pathogen control. Gene expression driven by phloem-derived Citrus sinensis gene promoters could be evaluated and compared with the expression induced by a strong constitutive promoter in the same tissue, leading to the production of transgenic sweet oranges potentially more resistant to diseases caused by phloem-limited bacteria. ?Carrizo? citrange [Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck] was transformed, via Agrobacterium tumefaciens, with the binary vector pCAMBIA2301 bearing the uidA gene (?-glucuronidase) driven by the CaMV35S constitutive promoter (CaMV35S::uidA) or by the CsPP2.B1 (CsPP2.B1::uidA) or by the CsVTE2 (CsVTE2::uidA) citrus promoters. In vitro regenerated shoots were grafted onto ?Rangpur? lime (C. limonia Osbeck). The genetic transformation was confirmed by Southern blot analyses. uidA gene expression was evaluated by RT-qPCR, and gene histolocalization controlled by these three promoters was accessed by X-GLUC treated stem sections. uidA gene expression exhibited by tissue-specific promoters was overall lower than from the constitutive promoter CaMV35; however, constructs driven by tissuespecific promoters may lead to expression in restricted tissues. CsPP2.B1 and CsVTE2 promoters can be considered adequate for the utilization in gene constructs aiming disease resistance.
Thesagro: | Fruta Cítrica.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/227820/1/How-to-drive-phloem-gene-expression.pdf
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (33417-1) - UPC - AP - DD - Publicação digital -  -  - DIS