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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
30/12/2019 |
Data da última atualização: |
06/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PILLAI, V. V.; SIQUEIRA, L. G. B.; DAS, M.; KEI, T. G.; TU, L. N.; HERREN, A. W.; PHINNEY, B. S.; CHEONG, S. H.; HANSEN, P. J.; SELVARAJ, V. |
Afiliação: |
VIJU VIJAYAN PILLAI, Cornell University, Ithaca, NY; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL; MOUBANI DAS, Cornell University, Ithaca, NY; TIFFANY G. KEI, Cornell University, Ithaca, NY; LAN N. TU, Cornell University, Ithaca, NY; ANTHONY W. HERREN, University of California; BRETT S. PHINNEY, University of California; SOON HON CHEONG, Cornell University, Ithaca, NY; PETER J. HANSEN, University of Florida, Gainesville, FL; VIMAL SELVARAJ, Cornell University, Ithaca, NY. |
Título: |
Physiological profile of undifferentiated bovine blastocyst-derived trophoblasts. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Biology Open, v. 8, n. 5, article bio037937, 2019. |
DOI: |
https://doi.org/10.1242/bio.037937 |
Idioma: |
Inglês |
Conteúdo: |
Trophectoderm of blastocysts mediate early events in fetal-maternal communication, enabling implantation and establishment of a functional placenta. Inadequate or impaired developmental events linked to trophoblasts directly impact early embryo survival and successful implantation during a crucial period that corresponds with high incidence of pregnancy losses in dairy cows. As yet, the molecular basis of bovine trophectoderm development and signaling towards initiation of implantation remains poorly understood. In this study, we developed methods for culturing undifferentiated bovine blastocyst-derived trophoblasts and used both transcriptomics and proteomics in early colonies to categorize and elucidate their functional characteristics. A total of 9270 transcripts and 1418 proteins were identified and analyzed based on absolute abundance. We profiled an extensive list of growth factors, cytokines and other relevant factors that can effectively influence paracrine communication in the uterine microenvironment. Functional categorization and analysis revealed novel information on structural organization, extracellular matrix composition, cell junction and adhesion components, transcription networks, and metabolic preferences. Our data showcase the fundamental physiology of bovine trophectoderm and indicate hallmarks of the self-renewing undifferentiated state akin to trophoblast stem cells described in other species. Functional features uncovered are essential for understanding early events in bovine pregnancy towards initiation of implantation. MenosTrophectoderm of blastocysts mediate early events in fetal-maternal communication, enabling implantation and establishment of a functional placenta. Inadequate or impaired developmental events linked to trophoblasts directly impact early embryo survival and successful implantation during a crucial period that corresponds with high incidence of pregnancy losses in dairy cows. As yet, the molecular basis of bovine trophectoderm development and signaling towards initiation of implantation remains poorly understood. In this study, we developed methods for culturing undifferentiated bovine blastocyst-derived trophoblasts and used both transcriptomics and proteomics in early colonies to categorize and elucidate their functional characteristics. A total of 9270 transcripts and 1418 proteins were identified and analyzed based on absolute abundance. We profiled an extensive list of growth factors, cytokines and other relevant factors that can effectively influence paracrine communication in the uterine microenvironment. Functional categorization and analysis revealed novel information on structural organization, extracellular matrix composition, cell junction and adhesion components, transcription networks, and metabolic preferences. Our data showcase the fundamental physiology of bovine trophectoderm and indicate hallmarks of the self-renewing undifferentiated state akin to trophoblast stem cells described in other species. Functional features uncovered are essential for understandi... Mostrar Tudo |
Thesagro: |
Placenta. |
Thesaurus Nal: |
Blastocyst; Embryo implantation; Pregnancy; Stem cells; Trophoblast. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/207915/1/BiolOpen-LGustavoSiq-Physiological-bio037937.full.pdf
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Marc: |
LEADER 02435naa a2200313 a 4500 001 2117829 005 2024-02-06 008 2019 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1242/bio.037937$2DOI 100 1 $aPILLAI, V. V. 245 $aPhysiological profile of undifferentiated bovine blastocyst-derived trophoblasts.$h[electronic resource] 260 $c2019 520 $aTrophectoderm of blastocysts mediate early events in fetal-maternal communication, enabling implantation and establishment of a functional placenta. Inadequate or impaired developmental events linked to trophoblasts directly impact early embryo survival and successful implantation during a crucial period that corresponds with high incidence of pregnancy losses in dairy cows. As yet, the molecular basis of bovine trophectoderm development and signaling towards initiation of implantation remains poorly understood. In this study, we developed methods for culturing undifferentiated bovine blastocyst-derived trophoblasts and used both transcriptomics and proteomics in early colonies to categorize and elucidate their functional characteristics. A total of 9270 transcripts and 1418 proteins were identified and analyzed based on absolute abundance. We profiled an extensive list of growth factors, cytokines and other relevant factors that can effectively influence paracrine communication in the uterine microenvironment. Functional categorization and analysis revealed novel information on structural organization, extracellular matrix composition, cell junction and adhesion components, transcription networks, and metabolic preferences. Our data showcase the fundamental physiology of bovine trophectoderm and indicate hallmarks of the self-renewing undifferentiated state akin to trophoblast stem cells described in other species. Functional features uncovered are essential for understanding early events in bovine pregnancy towards initiation of implantation. 650 $aBlastocyst 650 $aEmbryo implantation 650 $aPregnancy 650 $aStem cells 650 $aTrophoblast 650 $aPlacenta 700 1 $aSIQUEIRA, L. G. B. 700 1 $aDAS, M. 700 1 $aKEI, T. G. 700 1 $aTU, L. N. 700 1 $aHERREN, A. W. 700 1 $aPHINNEY, B. S. 700 1 $aCHEONG, S. H. 700 1 $aHANSEN, P. J. 700 1 $aSELVARAJ, V. 773 $tBiology Open$gv. 8, n. 5, article bio037937, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Acre. |
Data corrente: |
16/08/1996 |
Data da última atualização: |
28/06/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Nacional - A |
Autoria: |
CAMPOS, I. S.; ASSUNÇÃO, M. V. |
Afiliação: |
Ivandir Soares Campos, Embrapa Acre; Marcos Vinícius Assunção, Universidade Federal do Ceará (UFC). |
Título: |
Efeitos do cloreto de sódio na germinação e vigor de plântulas de arroz. |
Ano de publicação: |
1990 |
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 25, n. 6, p. 837-843, jun. 1990. |
ISSN: |
0100-204X (impresso) / 1678-3921 (online) |
Idioma: |
Português |
Conteúdo: |
Este trabalho teve como objetivo avaliar os efeitos delet&ios do NaU na germinação e vigor das cultivares de arroz (Oryza saliva L.) IAC-25, IAC-47, IAC-165, CNA-796019, Lebonnet, IRAT-112 e CICA-8. Amostras de sementes foram colocadas para germinar em solução de NaU com potenciais osmóticos de - 0,4 e - 0,8 Megapascal (MPa). As cultivares IAC-25 e IAC-47 foram consideradas tolerantes à salinidade atá - 0,4 MPa de NaCl; Lebonnet, CNA-796019 e CICA-8 foram consideradas moderadamente tolerantes; a IAC-165 e IRAT-112 pouco tolerantes. Entretanto, a -0,8 MPa, em que todo material foi severamente afetado pela salinidade, as cultivares IAC-25, IAC-47 e CNA-796019 foram as menos sensíveis. A cultivar IAC-25, de maneira geral, foi a mais tolerante à salinidade imposta e a IAC-165 foi a mais sensíveL |
Palavras-Chave: |
Germinación de las semillas; Oryza sativa L; Salinidad del suelo. |
Thesagro: |
Arroz; Características Agronômicas; Cloreto de sódio; Germinação; Solo salino; Teste de vigor; Variedade resistente. |
Thesaurus NAL: |
Agronomic traits; Rice; Seed germination; Soil salinity. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/112453/1/899.pdf
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Marc: |
LEADER 01768naa a2200313 a 4500 001 1492146 005 2021-06-28 008 1990 bl uuuu u00u1 u #d 022 $a0100-204X (impresso) / 1678-3921 (online) 100 1 $aCAMPOS, I. S. 245 $aEfeitos do cloreto de sódio na germinação e vigor de plântulas de arroz.$h[electronic resource] 260 $c1990 520 $aEste trabalho teve como objetivo avaliar os efeitos delet&ios do NaU na germinação e vigor das cultivares de arroz (Oryza saliva L.) IAC-25, IAC-47, IAC-165, CNA-796019, Lebonnet, IRAT-112 e CICA-8. Amostras de sementes foram colocadas para germinar em solução de NaU com potenciais osmóticos de - 0,4 e - 0,8 Megapascal (MPa). As cultivares IAC-25 e IAC-47 foram consideradas tolerantes à salinidade atá - 0,4 MPa de NaCl; Lebonnet, CNA-796019 e CICA-8 foram consideradas moderadamente tolerantes; a IAC-165 e IRAT-112 pouco tolerantes. Entretanto, a -0,8 MPa, em que todo material foi severamente afetado pela salinidade, as cultivares IAC-25, IAC-47 e CNA-796019 foram as menos sensíveis. A cultivar IAC-25, de maneira geral, foi a mais tolerante à salinidade imposta e a IAC-165 foi a mais sensíveL 650 $aAgronomic traits 650 $aRice 650 $aSeed germination 650 $aSoil salinity 650 $aArroz 650 $aCaracterísticas Agronômicas 650 $aCloreto de sódio 650 $aGerminação 650 $aSolo salino 650 $aTeste de vigor 650 $aVariedade resistente 653 $aGerminación de las semillas 653 $aOryza sativa L 653 $aSalinidad del suelo 700 1 $aASSUNÇÃO, M. V. 773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 25, n. 6, p. 837-843, jun. 1990.
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