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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
15/04/2004 |
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Data da última atualização: |
15/04/2004 |
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Autoria: |
RAEMAKERS, C. C. J. M.; ROZEBOOM, M.; JACOBSEN, E.; VISSER, R. G. F. |
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Título: |
Production of transgenic plants by partcile bombardment, electroporation and Agrobacterium tumefaciens. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Revista Brasileira de Mandioca, Cruz das Almas, v. 17, p. 33, nov., 1998. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Since the first production of transgenic plants by particle bombardment in 1996 the method has been constantly improved and new methods were developed. The luciferase selection method used for the production of the first transgenics had as disadvantage that only a fraction of the tissue was transgenic. The luciferase selection method was refined leading to complete transgenic cultures. Furthermore, transgenic plants have been produced via combination of luciferase selection with electroporation and via combination of kanamycin and hygromycin selection with Agrobacterium tumefaciens. The transformation methods will be presented and compared with each other with respect to time and labour required using a model. Also the effect of some improved methods of regeneration of plants from FEC are presented using this model. Furthermore, the morphology of the transgenic will be compared with control regenerants and control plants. |
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Categoria do assunto: |
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Marc: |
LEADER 01457naa a2200157 a 4500
001 1651908
005 2004-04-15
008 1998 bl uuuu u00u1 u #d
100 1 $aRAEMAKERS, C. C. J. M.
245 $aProduction of transgenic plants by partcile bombardment, electroporation and Agrobacterium tumefaciens.
260 $c1998
520 $aAbstract: Since the first production of transgenic plants by particle bombardment in 1996 the method has been constantly improved and new methods were developed. The luciferase selection method used for the production of the first transgenics had as disadvantage that only a fraction of the tissue was transgenic. The luciferase selection method was refined leading to complete transgenic cultures. Furthermore, transgenic plants have been produced via combination of luciferase selection with electroporation and via combination of kanamycin and hygromycin selection with Agrobacterium tumefaciens. The transformation methods will be presented and compared with each other with respect to time and labour required using a model. Also the effect of some improved methods of regeneration of plants from FEC are presented using this model. Furthermore, the morphology of the transgenic will be compared with control regenerants and control plants.
700 1 $aROZEBOOM, M.
700 1 $aJACOBSEN, E.
700 1 $aVISSER, R. G. F.
773 $tRevista Brasileira de Mandioca, Cruz das Almas$gv. 17, p. 33, nov., 1998. Suplemento.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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