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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
31/01/2013 |
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Data da última atualização: |
24/04/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
EIRAS, M.; SILVA, S. R.; STUCHI, E. S.; CARVALHO, S. A.; GARCÊZ, R. M. |
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Afiliação: |
MARCELO EIRAS, Centro de Pesquisa e Desenvolvimento de Sanidade Vegetal; SIMONE R. SILVA, ESALQ; EDUARDO SANCHES STUCHI, CNPMF; SÉRGIO A. CARVALHO, IAC; RENATA M. GARCÊZ, Centro de Pesquisa e Desenvolvimento de Sanidade Vegetal. |
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Título: |
Identification and characterization of viroids in Navelina ISA 315 sweet Orange. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v. 38, n. 1, p. 58-62, 2013. |
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Idioma: |
Inglês |
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Conteúdo: |
To date, seven species of viroids have been described infecting Citrus. However, only Citrus exocortis viroid (CEVd), Hop stunt viroid (HSVd) and Citrus dwarfing viroid (CDVd) have been reported in this crop in Brazil. The aim of our work was to identify and characterize viroids infecting plants of 'Navelina ISA 315' sweet orange cultivar grafted on 'Rangpur' lime from field experiments located in Bebedouro, SP, that showed gumming in the bark and wood symptoms. Biological indexing was done on the indicator host 'Clemelin 1120' tangor plants that reacted with typical gumming symptoms. Viroid infection was assessed by graft-inoculation of buds from the field trees on 'Etrog' citron plants grafted on 'Rangpur' lime, followed by RNA extraction and sPAGE analysis. RNAs were subjected to RTPCR with primers for citrus viroids. The full-length amplified products were sequenced and compared to those available in the GenBank. The trees were found to be infected with cachexia (Ca) variants of HSVd-Ca or HSVd-Ca plus CDVd. The results indicate that efforts have to be made to increase and stimulate the indexing programs, to maintain plants healthy and to develop sanitary programs focused on reducing the spread of viroids and other graft-transmissible agents. |
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Thesaurus Nal: |
viroids. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01766naa a2200181 a 4500
001 1947183
005 2013-04-24
008 2013 bl uuuu u00u1 u #d
100 1 $aEIRAS, M.
245 $aIdentification and characterization of viroids in Navelina ISA 315 sweet Orange.
260 $c2013
520 $aTo date, seven species of viroids have been described infecting Citrus. However, only Citrus exocortis viroid (CEVd), Hop stunt viroid (HSVd) and Citrus dwarfing viroid (CDVd) have been reported in this crop in Brazil. The aim of our work was to identify and characterize viroids infecting plants of 'Navelina ISA 315' sweet orange cultivar grafted on 'Rangpur' lime from field experiments located in Bebedouro, SP, that showed gumming in the bark and wood symptoms. Biological indexing was done on the indicator host 'Clemelin 1120' tangor plants that reacted with typical gumming symptoms. Viroid infection was assessed by graft-inoculation of buds from the field trees on 'Etrog' citron plants grafted on 'Rangpur' lime, followed by RNA extraction and sPAGE analysis. RNAs were subjected to RTPCR with primers for citrus viroids. The full-length amplified products were sequenced and compared to those available in the GenBank. The trees were found to be infected with cachexia (Ca) variants of HSVd-Ca or HSVd-Ca plus CDVd. The results indicate that efforts have to be made to increase and stimulate the indexing programs, to maintain plants healthy and to develop sanitary programs focused on reducing the spread of viroids and other graft-transmissible agents.
650 $aviroids
700 1 $aSILVA, S. R.
700 1 $aSTUCHI, E. S.
700 1 $aCARVALHO, S. A.
700 1 $aGARCÊZ, R. M.
773 $tTropical Plant Pathology$gv. 38, n. 1, p. 58-62, 2013.
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