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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
09/11/2004 |
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Data da última atualização: |
23/11/2015 |
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Autoria: |
CARVALHO, C. H. S.; ZEHR, U. B.; GUNARATNA, N.; ANDERSON, J.; KONONOWICZ, H. H.; HODGES, T. K.; AXTELL, J. D. |
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Afiliação: |
CARLOS HENRIQUE S DE CARVALHO, Embrapa Milho e Sorgo; Usha B. Zehr, Purdue University; Nilupa Gunaratna, Purdue University; Joseph Anderson, Purdue University; Halina H. Kononowicz, Purdue University; Thomas K. Hodges, Purdue University; John D. Axtell, Purdue University. |
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Título: |
Agrobacterium-mediated transformation of sorghum: factors that affect transformation efficiency. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Genetics and Molecular Biology, Ribeirão Preto, v. 27, n. 2, p. 259-269, 2004. |
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Idioma: |
Inglês |
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Conteúdo: |
The results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculum and the selection of sorghum genotypes and explants less sensitive to Agrobacterium infection. MenosThe results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculu... Mostrar Tudo |
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Thesagro: |
Sorgo. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32593/1/Agrobacterium-mediated.pdf
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Marc: |
LEADER 02225naa a2200205 a 4500
001 1488374
005 2015-11-23
008 2004 bl uuuu u00u1 u #d
100 1 $aCARVALHO, C. H. S.
245 $aAgrobacterium-mediated transformation of sorghum$bfactors that affect transformation efficiency.$h[electronic resource]
260 $c2004
520 $aThe results presented in this work support the hypothesis that Agrobacterium-mediated transformation of sorghum is feasible, analogous to what has been demonstrated for other cereals such as rice, maize, barley and wheat. The four factors that we found most influenced transformation were: the sensitivity of immature sorghum embryos to Agrobacterium infection, the growth conditions of the donor plant, type of explant and co-cultivation medium. A major problem during the development of our protocol was a necrotic response which developed in explants after co-cultivation. Immature sorghum embryos proved to be very sensitive to Agrobacterium infection and we found that the level of embryo death after co-cultivation was the limiting step in improving transformation efficiency. The addition of coconut water to the co-cultivation medium, the use of vigorous and actively growing immature embryos and the removal of excess bacteria significantly improved the survival rate of sorghum embryos and was critical for successful transformation. Hygromycin phosphotransferase (hpt) proved to be a good selectable marker for sorghum. We also found that β-glucuronidase (GUS) activity was low in most of the transgenic plant tissues tested, although it was very high in immature inflorescences. Although promising, the overall transformation efficiency of the protocol is still low and further optimization will require particular attention to be given to the number of Agrobacterium in the inoculum and the selection of sorghum genotypes and explants less sensitive to Agrobacterium infection.
650 $aSorgo
700 1 $aZEHR, U. B.
700 1 $aGUNARATNA, N.
700 1 $aANDERSON, J.
700 1 $aKONONOWICZ, H. H.
700 1 $aHODGES, T. K.
700 1 $aAXTELL, J. D.
773 $tGenetics and Molecular Biology, Ribeirão Preto$gv. 27, n. 2, p. 259-269, 2004.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Classificação |
Cutter |
Registro |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
15/10/2019 |
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Data da última atualização: |
16/10/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SCHEPASCHENKO, D.; CHAVE, J.; PHILLIPS, O. L.; LEWIS, S. L.; DAVIES, S. J.; RÉJOU-MÉCHAIN, M.; SIST, P.; SCIPAL, K.; PERGER, C.; HERAULT, B.; LABRIÈRE, N.; HOFHANSL, F.; AFFUM-BAFFOE, K.; ALEINIKOV, A.; ALONSO, A.; AMANI, C.; ARAUJO-MURAKAMI, A.; ARMSTON, J.; ARROYO, L.; ASCARRUNZ, N.; AZEVEDO, C. P. de; BAKER, T.; BALAZY, R.; BEDEAU, C.; BERRY, N.; BILOUS, A. M.; BILOUS, S. Y.; BISSIENGOU, P.; BLANC, L.; BOBKOVA, K. S.; BRASLAVSKAYA, T.; BRIENEN, R.; BURSLEM, D. F. R. P.; CONDIT, R.; CUNI-SANCHEZ, A.; DANILINA, D.; TORRES, D. del C.; DERROIRE, G.; DESCROIX, L.; SOTTA, E. D.; OLIVEIRA, M. V. N. d'; DRESEL, C.; ERWIN, T.; EVDOKIMENKO, M. D.; FALCK, J.; FELDPAUSCH, T. R.; FOLI, E. G.; FOSTER, R.; FRITZ, S.; GARCIA-ABRIL, A. D.; GORNOV, A.; GORNOVA, M.; GOTHARD-BASSÉBÉ, E.; GOURLET-FLEURY, S.; GUEDES, M. C.; HAMER, K. C.; SUSANTY, F. H.; HIGUCHI, N.; CORONADO, E. N. H.; HUBAU, W.; HUBBELL, S.; ILSTEDT, U.; IVANOV, V. V.; KANASHIRO, M.; KARLSSON, A.; KARMINOV, V. N.; KILLEEN, T.; KOFFI, J. C. K.; KONOVALOVA, M.; KRAXNER, F.; KREJZA, J.; KRISNAWATI, H.; KRIVOBOKOV, L. V.; KUZNETSOV, M. A.; LAKYDA, I.; LAKYDA, P. I.; LICONA, J. C.; LUCAS, R. M.; LUKINA, N.; LUSSETTI, D.; MALHI, Y.; MANZANERA, J. A.; MARIMON, B.; MARIMON JUNIOR, B. H.; VASQUEZ MARTINEZ, R.; MARTYNENKO, O. V.; MATSALA, M.; MATYASHUK, R. K.; FREITAS, L. J. M. de; MEMIAGHE, H.; MENDOZA, C.; MONTEAGUDO MENDONZA, A.; MOROZIUK, O. V.; MUKHORTOVA, L.; MUSA, S.; NAZIMOVA, D. I.; OKUDA, T.; OLIVEIRA, L. C. de; ONTIKOV, P. V.; OSIPOV, A. F.; PIETSCH, S.; PLAYFAIR, M.; POULSEN, J.; RADCHENKO, V. G.; RODNEY, K.; ROZAK, A. H.; RUSCHEL, A. R.; RUTISHAUSER, E.; SEE, L.; SHCHEPASHCHENKO, M.; SHEVCHENKO, N.; SHVIDENKO, A.; SILVEIRA, M.; SINGH, J.; SONKÉ, B.; SOUZA, C. R. de; STERENCZAK, K.; STONOZHENKO, L.; SULLIVAN, M. J. P.; SZATNIEWSKA, J.; TAEDOUMG, H.; TER STEEGE, H.; TIKHONOVA, E.; TOLEDO, M.; TREFILOVA, O. V.; VALBUENA, R.; VALENZUELA GAMARRA, L.; VASILIEV, S.; VEDROVA, E. F.; VERHOVETS, S. V.; VIDAL, E.; VLADIMIROVA, N. A.; VLEMINCKX, J.; VOS, V. A.; VOZMITEL, F. K.; WANEK, W.; WEST, T. A. P.; WOELL, H.; WOODS, J. T.; WORTEL, V.; YAMADA, T.; HAJAR, Z. S. N.; ZO-BI, I. C. |
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Afiliação: |
DMITRY SCHEPASCHENKO; JÉRÔME CHAVE; OLIVER L. PHILLIPS; SIMON L. LEWIS; STUART J. DAVIES; MAXIME RÉJOU-MÉCHAIN; PLINIO SIST; KLAUS SCIPAL; CHRISTOPH PERGER; BRUNO HERAULT; NICOLAS LABRIÈRE; FLORIAN HOFHANSL; KOFI AFFUM-BAFFOE; ALEXEI ALEINIKOV; ALFONSO ALONSO; CHRISTIAN AMANI; ALEJANDRO ARAUJO-MURAKAMI; JOHN ARMSTON; LUZMILA ARROYO; NATALY ASCARRUNZ; CELSO PAULO DE AZEVEDO, CPAA; TIMOTHY BAKER; RADOMIR BALAZY; CAROLINE BEDEAU; NICHOLAS BERRY; ANDRII M. BILOUS; SVITLANA YU. BILOUS; PULCHÉRIE BISSIENGOU; LILIAN BLANC; KAPITOLINA S. BOBKOVA; TATYANA BRASLAVSKAYA; ROEL BRIENEN; DAVID F. R. P. BURSLEM; RICHARD CONDIT; AIDA CUNI-SANCHEZ; DILSHAD DANILINA; DENNIS DEL CASTILLO TORRES; GÉRALDINE DERROIRE; LAURENT DESCROIX; ELENEIDE DOFF SOTTA; MARCUS VINICIO NEVES D OLIVEIRA, CPAF-AC; CHRISTOPHER DRESEL; TERRY ERWIN; MIKHAIL D. EVDOKIMENKO; JAN FALCK; TED R. FELDPAUSCH; ERNEST G. FOLI; ROBIN FOSTER; STEFFEN FRITZ; ANTONIO DAMIAN GARCIA-ABRIL; ALEKSEY GORNOV; MARIA GORNOVA; ERNEST GOTHARD-BASSÉBÉ; SYLVIE GOURLET-FLEURY; MARCELINO CARNEIRO GUEDES, CPAF-AP; KEITH C. HAMER; FARIDA HERRY SUSANTY; NIRO HIGUCHI; EURÍDICE N. HONORIO CORONADO; WANNES HUBAU; STEPHEN HUBBELL; ULRIK ILSTEDT; VIKTOR V. IVANOV; MILTON KANASHIRO, CPATU; ANDERS KARLSSON; VIKTOR N. KARMINOV; TIMOTHY KILLEEN; JEAN-CLAUDE KONAN KOFFI; MARIA KONOVALOVA; FLORIAN KRAXNER; JAN KREJZA; HARUNI KRISNAWATI; LEONID V. KRIVOBOKOV; MIKHAIL A. KUZNETSOV; IVAN LAKYDA; PETRO I. LAKYDA; JUAN CARLOS LICONA; RICHARD M. LUCAS; NATALIA LUKINA; DANIEL LUSSETTI; YADVINDER MALHI; JOSÉ ANTONIO MANZANERA; BEATRIZ MARIMON; BEN HUR MARIMON JUNIOR; RODOLFO VASQUEZ MARTINEZ; OLGA V. MARTYNENKO; MAKSYM MATSALA; RAISA K. MATYASHUK; LUCAS JOSE MAZZEI DE FREITAS, CPATU; HERVÉ MEMIAGHE; CASIMIRO MENDOZA; ABEL MONTEAGUDO MENDOZA; OLGA V. MOROZIUK; LIUDMILA MUKHORTOVA; SAMSUDIN MUSA; DINA I. NAZIMOVA; TOSHINORI OKUDA; LUIS CLAUDIO DE OLIVEIRA, CPAF-AC; PETR V. ONTIKOV; ANDREY F. OSIPOV; STEPHAN PIETSCH; MAUREEN PLAYFAIR; JOHN POULSEN; VLADIMIR G. RADCHENKO; KENNETH RODNEY; ANDES H. ROZAK; ADEMIR ROBERTO RUSCHEL, CPATU; ERVAN RUTISHAUSER; LINDA SEE; MARIA SHCHEPASHCHENKO; NIKOLAY SHEVCHENKO; ANATOLY SHVIDENKO; MARCOS SILVEIRA; JAMES SINGH; BONAVENTURE SONKÉ; CINTIA RODRIGUES DE SOUZA, CPAA; KRZYSZTOF STERENCZAK; LEONID STONOZHENKO; MARTIN J. P. SULLIVAN; JUSTYNA SZATNIEWSKA; HERMANN TAEDOUMG; HANS TER STEEGE; ELENA TIKHONOVA; MARISOL TOLEDO; OLGA V. TREFILOVA; RUBEN VALBUENA; LUIS VALENZUELA GAMARRA; SERGEY VASILIEV; ESTELLA F. VEDROVA; SERGEY V. VERHOVETS; EDSON VIDAL; NADEZHDA A. VLADIMIROVA; JASON VLEMINCKX; VINCENT A. VOS; FOMA K. VOZMITEL; WOLFGANG WANEK; THALES A. P. WEST; HANNSJORG WOELL; JOHN T. WOODS; VERGINIA WORTEL; TOSHIHIRO YAMADA; ZAMAH SHARI NUR HAJAR; IRIÉ CASIMIR ZO-BI. |
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Título: |
The forest observation system, building a global reference dataset for remote sensing of forest biomass. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Scientific Data, v. 6, n. 198, p. 1-11, 2019. |
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DOI: |
https://doi.org/10.1038/s41597-019-0196-1 |
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Idioma: |
Inglês |
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Conteúdo: |
Forest biomass is an essential indicator for monitoring the Earth?s ecosystems and climate. It is a critical input to greenhouse gas accounting, estimation of carbon losses and forest degradation, assessment of renewable energy potential, and for developing climate change mitigation policies such as REDD+, among others. |
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Thesagro: |
Biomassa; Sensoriamento Remoto. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/202898/1/Nature-Schepaschenko-Biomassa.pdf
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Marc: |
LEADER 05124naa a2201861 a 4500
001 2113119
005 2019-10-16
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/s41597-019-0196-1$2DOI
100 1 $aSCHEPASCHENKO, D.
245 $aThe forest observation system, building a global reference dataset for remote sensing of forest biomass.$h[electronic resource]
260 $c2019
520 $aForest biomass is an essential indicator for monitoring the Earth?s ecosystems and climate. It is a critical input to greenhouse gas accounting, estimation of carbon losses and forest degradation, assessment of renewable energy potential, and for developing climate change mitigation policies such as REDD+, among others.
650 $aBiomassa
650 $aSensoriamento Remoto
700 1 $aCHAVE, J.
700 1 $aPHILLIPS, O. L.
700 1 $aLEWIS, S. L.
700 1 $aDAVIES, S. J.
700 1 $aRÉJOU-MÉCHAIN, M.
700 1 $aSIST, P.
700 1 $aSCIPAL, K.
700 1 $aPERGER, C.
700 1 $aHERAULT, B.
700 1 $aLABRIÈRE, N.
700 1 $aHOFHANSL, F.
700 1 $aAFFUM-BAFFOE, K.
700 1 $aALEINIKOV, A.
700 1 $aALONSO, A.
700 1 $aAMANI, C.
700 1 $aARAUJO-MURAKAMI, A.
700 1 $aARMSTON, J.
700 1 $aARROYO, L.
700 1 $aASCARRUNZ, N.
700 1 $aAZEVEDO, C. P. de
700 1 $aBAKER, T.
700 1 $aBALAZY, R.
700 1 $aBEDEAU, C.
700 1 $aBERRY, N.
700 1 $aBILOUS, A. M.
700 1 $aBILOUS, S. Y.
700 1 $aBISSIENGOU, P.
700 1 $aBLANC, L.
700 1 $aBOBKOVA, K. S.
700 1 $aBRASLAVSKAYA, T.
700 1 $aBRIENEN, R.
700 1 $aBURSLEM, D. F. R. P.
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700 1 $aTORRES, D. del C.
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700 1 $aDESCROIX, L.
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700 1 $aDRESEL, C.
700 1 $aERWIN, T.
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700 1 $aFOLI, E. G.
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700 1 $aFRITZ, S.
700 1 $aGARCIA-ABRIL, A. D.
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700 1 $aGOTHARD-BASSÉBÉ, E.
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700 1 $aKRIVOBOKOV, L. V.
700 1 $aKUZNETSOV, M. A.
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700 1 $aLAKYDA, P. I.
700 1 $aLICONA, J. C.
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700 1 $aMONTEAGUDO MENDONZA, A.
700 1 $aMOROZIUK, O. V.
700 1 $aMUKHORTOVA, L.
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700 1 $aNAZIMOVA, D. I.
700 1 $aOKUDA, T.
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700 1 $aOSIPOV, A. F.
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700 1 $aPLAYFAIR, M.
700 1 $aPOULSEN, J.
700 1 $aRADCHENKO, V. G.
700 1 $aRODNEY, K.
700 1 $aROZAK, A. H.
700 1 $aRUSCHEL, A. R.
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700 1 $aSHEVCHENKO, N.
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700 1 $aVLADIMIROVA, N. A.
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700 1 $aVOZMITEL, F. K.
700 1 $aWANEK, W.
700 1 $aWEST, T. A. P.
700 1 $aWOELL, H.
700 1 $aWOODS, J. T.
700 1 $aWORTEL, V.
700 1 $aYAMADA, T.
700 1 $aHAJAR, Z. S. N.
700 1 $aZO-BI, I. C.
773 $tScientific Data$gv. 6, n. 198, p. 1-11, 2019.
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