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| 8. |  | ARAUJO, F. R. Tuberculose bovina: sintomas, prejuízos, medidas preventivas. Campo Grande News, 12 de fev 2014, 1 p. Cassilândia News, 12 de fev. 2014; Jornal Agora, MS, 11 de fev, 2014; ZooNews O Portal da Informação do Agronegócio, 11 de fev, 2014; A Tribuna News, 14 de fev, 204| Biblioteca(s): Embrapa Gado de Corte. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Gado de Leite. |
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Data corrente: |
07/06/2023 |
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Data da última atualização: |
29/11/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
FERNANDEZ, Z.; RODRIGUES, R. de A.; TORRES, J. M.; MARCON, G. E. B.; FERREIRA, E. de C.; SOUZA, V. F. de; SARTI, E. F. B.; BERTOLLI, G. F.; ARAUJO, D.; DEMARCHI, L. H. F.; LICHS, G.; ZARDIN, M. U.; GONÇALVES, C. C. M.; CUENCA, V.; FAVACHO, A.; GUILHERMINO, J.; SANTOS, L. R. dos; ARAUJO, F. R.; SILVA, M. R. |
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Afiliação: |
ZORAIDA FERNANDEZ, Fundação Oswaldo Cruz; RUDIELLE DE ARRUDA RODRIGUES, Fundação Oswaldo Cruz; JAIRE MARINHO TORRES, Fundação Oswaldo Cruz; GLAUCIA ELISETE BARBOSA MARCON, Fundação Oswaldo Cruz; EDUARDO DE CASTRO FERREIRA, Fundação Oswaldo Cruz; VANESSA FELIPE DE SOUZA, CNPGC; ELAINE FERNANDES BAEZ SARTI, Universidade Federal de Mato Grosso do Sul; GUILHERME FERMINAO BERTOLLI, Universidade Federal de Mato Grosso do Sul; DANIEL ARAUJO, Universidade Federal de Mato Grosso do Sul; LUIZ HENRIQUE FERRAZ DEMARCHI, Laboratório Central de Saúde do Mato Grosso do Sul; GISLENE LICHS, Laboratório Central de Saúde do Mato Grosso do Sul; MARINA UMAKI ZARDIN, Laboratório Central de Saúde do Mato Grosso do Sul; CRHISTINNE CAVALHEIRO MAYMONE GONÇALVES, Secretaria de Estado de Saúde do Mato Grosso do Sul; VALTER CUENCA, Fundação Universidade Federal de Mato Grosso do Sul; ALEXSANDRA FAVACHO, Fundação Oswaldo Cruz; JISLAINE GUILHERMINO, Fundação Oswaldo Cruz; LENITA RAMIRES DOS SANTOS, CNPGC; FLABIO RIBEIRO DE ARAUJO, CNPGC; MARCIO ROBERTO SILVA, CNPGL. |
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Título: |
Development and validity assessment of ELISA test with recombinant chimeric protein of SARS-CoV-2. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Journal of Immunological Methods, v. 519, e113489, 2023. |
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DOI: |
https://doi.org/10.1016/j.jim.2023.113489 |
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Idioma: |
Inglês |
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Conteúdo: |
Serological tests developed for COVID-19 diagnostic are based on antibodies specific for SARS-CoV-2 antigens. Most of the antigens consist of a fragment or a whole amino acid sequence of the nucleocapsid or spike proteins. We evaluated a chimeric recombinant protein as an antigen in an ELISA test, using the most conserved and hydrophilic portions of the S1-subunit of the S and Nucleocapsid (N) proteins. These proteins, individually, indicated a suitable sensitivity of 93.6 and 100% and a specificity of 94.5 and 91.3%, respectively. However, our study with the chimera containing S1 and N proteins of SARS-CoV-2 suggested that the recombinant protein could better balance both the sensitivity (95.7%) and the specificity (95.5%) of the serological assay when comparing with the ELISA test using the antigens N and S1, individually. Accordingly, the chimera showed a high area under the ROC curve of 0.98 (CI 95% 0.958-1). Thus, our chimeric approach could be used to assess the natural exposure against SARS-CoV-2 virus over time, however, other tests will be necessary to better understand the behaviour of the chimera in samples from people with different vaccination doses and/or infected with different variants of the virus. |
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Palavras-Chave: |
Chimeric protein; COVID 19; Diagnostic; Proteína quimérica; Teste ELISA. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
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024 7 $ahttps://doi.org/10.1016/j.jim.2023.113489$2DOI
100 1 $aFERNANDEZ, Z.
245 $aDevelopment and validity assessment of ELISA test with recombinant chimeric protein of SARS-CoV-2.$h[electronic resource]
260 $c2023
520 $aSerological tests developed for COVID-19 diagnostic are based on antibodies specific for SARS-CoV-2 antigens. Most of the antigens consist of a fragment or a whole amino acid sequence of the nucleocapsid or spike proteins. We evaluated a chimeric recombinant protein as an antigen in an ELISA test, using the most conserved and hydrophilic portions of the S1-subunit of the S and Nucleocapsid (N) proteins. These proteins, individually, indicated a suitable sensitivity of 93.6 and 100% and a specificity of 94.5 and 91.3%, respectively. However, our study with the chimera containing S1 and N proteins of SARS-CoV-2 suggested that the recombinant protein could better balance both the sensitivity (95.7%) and the specificity (95.5%) of the serological assay when comparing with the ELISA test using the antigens N and S1, individually. Accordingly, the chimera showed a high area under the ROC curve of 0.98 (CI 95% 0.958-1). Thus, our chimeric approach could be used to assess the natural exposure against SARS-CoV-2 virus over time, however, other tests will be necessary to better understand the behaviour of the chimera in samples from people with different vaccination doses and/or infected with different variants of the virus.
653 $aChimeric protein
653 $aCOVID 19
653 $aDiagnostic
653 $aProteína quimérica
653 $aTeste ELISA
700 1 $aRODRIGUES, R. de A.
700 1 $aTORRES, J. M.
700 1 $aMARCON, G. E. B.
700 1 $aFERREIRA, E. de C.
700 1 $aSOUZA, V. F. de
700 1 $aSARTI, E. F. B.
700 1 $aBERTOLLI, G. F.
700 1 $aARAUJO, D.
700 1 $aDEMARCHI, L. H. F.
700 1 $aLICHS, G.
700 1 $aZARDIN, M. U.
700 1 $aGONÇALVES, C. C. M.
700 1 $aCUENCA, V.
700 1 $aFAVACHO, A.
700 1 $aGUILHERMINO, J.
700 1 $aSANTOS, L. R. dos
700 1 $aARAUJO, F. R.
700 1 $aSILVA, M. R.
773 $tJournal of Immunological Methods$gv. 519, e113489, 2023.
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