Data/hora: 26/09/2024 11:51:08

Biblioteca(s): 	Embrapa Milho e Sorgo.	
Data corrente: 	24/02/2015	
Data da última atualização: 	23/05/2017	
Autoria: 	BRAGA, R. M.; SANTANA, M. F.; COSTA, R. V. da; BROMMONSCHENKEL, S. H.; ARAÚJO, E. F. de; QUEIROZ, M. V. de.	
Afiliação: 	RODRIGO VERAS DA COSTA, CNPMS.	
Título: 	Transposable elements belonging to the Tc1-Mariner superfamily are heavily mutated in Colletotrichum graminicola.	
Ano de publicação: 	2014	
Fonte/Imprenta: 	Mycologia, New York, v. 106, n. 4, p. 629-641, 2014.	
DOI: 	10.3852/13?262	
Idioma: 	Inglês	
Conteúdo: 	Transposable elements are ubiquitous and constitute an important source of genetic variation in addition to generating deleterious mutations. Several filamentous fungi are able to defend against transposable elements using RIP(repeat-induced point mutation)-like mechanisms, which induce mutations in duplicated sequences. The sequenced Colletotrichum graminicola genome and the availability of transposable element databases provide an efficient approach for identifying and characterizing transposable elements in this fungus, which was the subject of this study. We identified 132 full-sized Tc1-Mariner transposable elements in the sequenced C. graminicola genome, which were divided into six families. Several putative transposases that have been found in these elements have conserved DDE motifs, but all are interrupted by stop codons. An in silico analysis showed evidence for RIP-generated mutations. The TCg1 element, which was cloned from the Brazilian 2908 m isolate, has a putative transposase sequence with three characteristic conserved motifs. However, this sequence is interrupted by five stop codons. Genomic DNA from various isolates was analyzed by hybridization with an internal region of TCg1. All of the isolates featured transposable elements that were similar to TCg1, and several hybridization profiles were identified. C. graminicola has many Tc1-Mariner transposable elements that have been degenerated by characteristic RIP mutations. It is unlikely that any of the characterized elements are autonomous in the sequenced isolate. The possible existence of active copies in field isolates from Brazil was shown. The TCg1 element is present in severalC. graminicola isolates and is a potentially useful molecular marker for population studies of this phytopathogen. Key words: RIP, transposase, transposon	
Thesagro: 	Antracnose.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Milho e Sorgo (CNPMS)	
Exemplares: 	CNPMS - (26375-1) - UPC - AP - DD -  -  -  - DIS