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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
08/12/2017 |
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Data da última atualização: |
08/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FIGUEIREDO, M. A. de; COELHO, S. V. B.; ROSA, S. D. V. F. da; VILELA, A. L.; SILVA, L. C. |
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Afiliação: |
MADELEINE ALVES DE FIGUEIREDO, DA/UFLA; STEFANIA VILAS BOAS COELHO, DA/UFLA; STTELA DELLYZETE VEIGA F DA ROSA, SAPC; AMANDA LIMA VILELA, DA/UFLA; LUCIANO COUTINHO SILVA, DBCM/UFPB. |
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Título: |
Exploratory |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Journal of Seed Science, v. 39, n. 2, p. 150-158, 2017 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Estudos exploratórios para a criopreservação de sementes de Coffea arabica L. |
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Conteúdo: |
Cryopreservation is a viable option for conservation of coffee germplasm. However, for this technique to be completely successful, it is of fundamental importance to carry out studies that ensure maintenance of cell integrity before and after immersion in liquid nitrogen (LN). Therefore, the aim in this study was to investigate the water content, cooling rate, and final temperature most suitable for cryopreservation of Coffea arabica L. seeds. The seeds were dried by silica gel to water contents of 5, 10, 15, 20, 30 and 40 % wb, subjected to slow cooling treatments at speeds of -1, -3 and -5 °C min.-1 to final temperatures of -40, -50 and -60 °C and then directly immersed in LN. After storage, seeds were rewarmed at 40 °C for two minutes. The survival rate and viability of the seeds and embryos were evaluated by the tetrazolium and germination tests. Results of the tetrazolium test indicate that embryos excised from cryopreserved seeds are less sensitive to cryopreservation than whole seeds are. The water content of 20% wb and the use of zygotic embryos led to the highest survival rate of the coffee seeds, depending on the cooling rate and the final temperature of precooling. |
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Palavras-Chave: |
Coffee; Liquid nitrogen; Nitrogênio líquido; Resfriamento lento; Slow cooling; Teor de água. |
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Thesagro: |
Café. |
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Thesaurus Nal: |
silica gel; Water content. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/168535/1/Exploratory-studies-for-cryopreservation.pdf
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Marc: |
LEADER 02030naa a2200289 a 4500
001 2081992
005 2017-12-08
008 2017 bl uuuu u00u1 u #d
100 1 $aFIGUEIREDO, M. A. de
245 $aExploratory$h[electronic resource]
260 $c2017
500 $aTítulo em português: Estudos exploratórios para a criopreservação de sementes de Coffea arabica L.
520 $aCryopreservation is a viable option for conservation of coffee germplasm. However, for this technique to be completely successful, it is of fundamental importance to carry out studies that ensure maintenance of cell integrity before and after immersion in liquid nitrogen (LN). Therefore, the aim in this study was to investigate the water content, cooling rate, and final temperature most suitable for cryopreservation of Coffea arabica L. seeds. The seeds were dried by silica gel to water contents of 5, 10, 15, 20, 30 and 40 % wb, subjected to slow cooling treatments at speeds of -1, -3 and -5 °C min.-1 to final temperatures of -40, -50 and -60 °C and then directly immersed in LN. After storage, seeds were rewarmed at 40 °C for two minutes. The survival rate and viability of the seeds and embryos were evaluated by the tetrazolium and germination tests. Results of the tetrazolium test indicate that embryos excised from cryopreserved seeds are less sensitive to cryopreservation than whole seeds are. The water content of 20% wb and the use of zygotic embryos led to the highest survival rate of the coffee seeds, depending on the cooling rate and the final temperature of precooling.
650 $asilica gel
650 $aWater content
650 $aCafé
653 $aCoffee
653 $aLiquid nitrogen
653 $aNitrogênio líquido
653 $aResfriamento lento
653 $aSlow cooling
653 $aTeor de água
700 1 $aCOELHO, S. V. B.
700 1 $aROSA, S. D. V. F. da
700 1 $aVILELA, A. L.
700 1 $aSILVA, L. C.
773 $tJournal of Seed Science$gv. 39, n. 2, p. 150-158, 2017
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Registro original: |
Embrapa Café (CNPCa) |
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