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Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
19/02/2016 |
Data da última atualização: |
15/04/2016 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
CASTELIANI, A. G. B.; KAVAMURA, V. N.; MORAIS, J. F. A.; SILVA, J. L. da; PANSA, C. C.; MELO, I. S. de. |
Afiliação: |
A. G. B. CASTELIANI, ESALQ/USP; V. N. KAVAMURA, ESALQ/USP; J. F. A. MORAIS, UNIP; J. L. da SILVA; C. C. PANSA, ESALQ/USP; ITAMAR SOARES DE MELO, CNPMA. |
Título: |
Bioprospecting biofilm-forming epiphytic bacteria from wheat under oligotrophic conditions. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 1655-1. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Naturally occurring epiphytic microorganisms display different colonization strategies. Studies report their potential in acting as biocontrol agents through several mechanisms such as competition for space and nutrients, production of antibiotics, resistance induction, parasitism, among others. However, little is known about the mechanisms that could possibly act in the control of the pathogenic fungus Magnaporthe grisea, responsible for wheat blast. Thus, the present work aimed the selection of epiphytic bacteria with the potential to act as biological control agents against M. grisea through different mechanisms of action. Bacterial isolates were subjected to direct antagonism tests. The growth rate in oligotrophic conditions (1% sucrose and 1% glucose) after 14 and 36 hours, biofilm production on 1% glucose and 1% sucrose and production of indole-3-acetic acid (IAA) were also tested. Thirty bacterial strains were isolated from wheat leaves (variety IAC 385) obtained from a field in Palmital, São Paulo state, Brazil. Any isolate showed direct antagonistic activity, indicating that no inhibition occurs through antibiosis. After 14 hours of growth, two strains (2EPPE3 and 1LCR10) exhibited better growth rate in medium supplemented with 1% sucrose. Biofilm production was favoured in one strain (1LCR3). More than 50 ?g.mL-1 of IAA was detected in one strain (1L10) and the production of this hormone might be related to the induction of defence response in plants. Isolates displaying the best growth rate in oligotrophic conditions and production of different compounds may be used together targeting a better biological control response against the plant pathogen. However, further analyses are necessary for verification of such mechanisms of action. MenosAbstract: Naturally occurring epiphytic microorganisms display different colonization strategies. Studies report their potential in acting as biocontrol agents through several mechanisms such as competition for space and nutrients, production of antibiotics, resistance induction, parasitism, among others. However, little is known about the mechanisms that could possibly act in the control of the pathogenic fungus Magnaporthe grisea, responsible for wheat blast. Thus, the present work aimed the selection of epiphytic bacteria with the potential to act as biological control agents against M. grisea through different mechanisms of action. Bacterial isolates were subjected to direct antagonism tests. The growth rate in oligotrophic conditions (1% sucrose and 1% glucose) after 14 and 36 hours, biofilm production on 1% glucose and 1% sucrose and production of indole-3-acetic acid (IAA) were also tested. Thirty bacterial strains were isolated from wheat leaves (variety IAC 385) obtained from a field in Palmital, São Paulo state, Brazil. Any isolate showed direct antagonistic activity, indicating that no inhibition occurs through antibiosis. After 14 hours of growth, two strains (2EPPE3 and 1LCR10) exhibited better growth rate in medium supplemented with 1% sucrose. Biofilm production was favoured in one strain (1LCR3). More than 50 ?g.mL-1 of IAA was detected in one strain (1L10) and the production of this hormone might be related to the induction of defence response in plants. Iso... Mostrar Tudo |
Palavras-Chave: |
Epiphytic bacteria; Wheat blast. |
Thesaurus Nal: |
Biological control. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/139525/1/2015RA-013.pdf
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Marc: |
LEADER 02516nam a2200205 a 4500 001 2037823 005 2016-04-15 008 2015 bl uuuu u00u1 u #d 100 1 $aCASTELIANI, A. G. B. 245 $aBioprospecting biofilm-forming epiphytic bacteria from wheat under oligotrophic conditions.$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 1655-1.$c1655 520 $aAbstract: Naturally occurring epiphytic microorganisms display different colonization strategies. Studies report their potential in acting as biocontrol agents through several mechanisms such as competition for space and nutrients, production of antibiotics, resistance induction, parasitism, among others. However, little is known about the mechanisms that could possibly act in the control of the pathogenic fungus Magnaporthe grisea, responsible for wheat blast. Thus, the present work aimed the selection of epiphytic bacteria with the potential to act as biological control agents against M. grisea through different mechanisms of action. Bacterial isolates were subjected to direct antagonism tests. The growth rate in oligotrophic conditions (1% sucrose and 1% glucose) after 14 and 36 hours, biofilm production on 1% glucose and 1% sucrose and production of indole-3-acetic acid (IAA) were also tested. Thirty bacterial strains were isolated from wheat leaves (variety IAC 385) obtained from a field in Palmital, São Paulo state, Brazil. Any isolate showed direct antagonistic activity, indicating that no inhibition occurs through antibiosis. After 14 hours of growth, two strains (2EPPE3 and 1LCR10) exhibited better growth rate in medium supplemented with 1% sucrose. Biofilm production was favoured in one strain (1LCR3). More than 50 ?g.mL-1 of IAA was detected in one strain (1L10) and the production of this hormone might be related to the induction of defence response in plants. Isolates displaying the best growth rate in oligotrophic conditions and production of different compounds may be used together targeting a better biological control response against the plant pathogen. However, further analyses are necessary for verification of such mechanisms of action. 650 $aBiological control 653 $aEpiphytic bacteria 653 $aWheat blast 700 1 $aKAVAMURA, V. N. 700 1 $aMORAIS, J. F. A. 700 1 $aSILVA, J. L. da 700 1 $aPANSA, C. C. 700 1 $aMELO, I. S. de
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
Data corrente: |
23/11/2017 |
Data da última atualização: |
09/04/2024 |
Tipo da produção científica: |
Nota Técnica/Nota Científica |
Autoria: |
PINTO, P. H. N.; BRAGANÇA, G. M.; BALARO, M. F. A.; ARASHIRO, E. K. N.; SANTOS, G. B.; SOUZA, G. N. de; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da; BRANDÃO, F. Z. |
Afiliação: |
Pedro Henrique Nicolau Pinto, Universidade Federal Fluminante (UFF) - Niterói, RJ; Gláucia Mota Bragança, UFF - Niterói, RJ; Mário Felipe Alvarez Balaro, UFF - Niterói, RJ; Eduardo Kenji Nunes Arashiro, UFF - Niterói, RJ; Gustavo Bervian dos Santos, UFF - Niterói, RJ; GUILHERME NUNES DE SOUZA, CNPGL; Joanna Maria Gonçalves Souza-Fabjan, UFF - Niterói, RJ; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Zandonade Brandão, UFF - Niterói, RJ. |
Título: |
Colour-Doppler ultrasound imaging as a laparoscopy substitute to count corpora lutea in superovulated sheep. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Reproduction in Domestic Animals, v. 53, n. 1, p. 266-269, Feb. 2018. |
Idioma: |
Inglês |
Conteúdo: |
Abstract: This study evaluated colour-Doppler ultrasound imaging (UI) as a substitute for laparoscopy to count the corpora lutea (CL) in superovulated sheep. Twenty-five Santa Ines ewes were superovulated three times at 21-day intervals. Corpora lutea were counted by colour-Doppler UI (CLDOPPLER ) 6 days after each superovulation and confirmed by laparoscopy (CLLAP ) 12 hr later. The mean number of CL was similar for both techniques (2.1 ± 2.5 vs. 2.1 ± 2.7 for CLDOPPLER and CLLAP , respectively) with a significant positive correlation (r = .94; r2 =.89). Colour-Doppler UI effectively evaluated the ovarian response in superovulated ewes and efficiently identified animals that did not respond to superovulation. |
Palavras-Chave: |
Colour-Doppler; Diagnostic imaging; Doppler ultrasound; Drug effects; Oestrus synchronization; Ovaries; Ultra-Som. |
Thesagro: |
Corpo lúteo; Laparoscopia; Ovário; Ovino; Reprodução animal; Sincronização do cio; Superovulação; Ultrassom. |
Thesaurus NAL: |
Corpus luteum; Laparoscopy; Reproduction; Sheep; Superovulation; Ultrasonography. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02044naa a2200469 a 4500 001 2080607 005 2024-04-09 008 2018 bl uuuu u00u1 u #d 100 1 $aPINTO, P. H. N. 245 $aColour-Doppler ultrasound imaging as a laparoscopy substitute to count corpora lutea in superovulated sheep.$h[electronic resource] 260 $c2018 520 $aAbstract: This study evaluated colour-Doppler ultrasound imaging (UI) as a substitute for laparoscopy to count the corpora lutea (CL) in superovulated sheep. Twenty-five Santa Ines ewes were superovulated three times at 21-day intervals. Corpora lutea were counted by colour-Doppler UI (CLDOPPLER ) 6 days after each superovulation and confirmed by laparoscopy (CLLAP ) 12 hr later. The mean number of CL was similar for both techniques (2.1 ± 2.5 vs. 2.1 ± 2.7 for CLDOPPLER and CLLAP , respectively) with a significant positive correlation (r = .94; r2 =.89). Colour-Doppler UI effectively evaluated the ovarian response in superovulated ewes and efficiently identified animals that did not respond to superovulation. 650 $aCorpus luteum 650 $aLaparoscopy 650 $aReproduction 650 $aSheep 650 $aSuperovulation 650 $aUltrasonography 650 $aCorpo lúteo 650 $aLaparoscopia 650 $aOvário 650 $aOvino 650 $aReprodução animal 650 $aSincronização do cio 650 $aSuperovulação 650 $aUltrassom 653 $aColour-Doppler 653 $aDiagnostic imaging 653 $aDoppler ultrasound 653 $aDrug effects 653 $aOestrus synchronization 653 $aOvaries 653 $aUltra-Som 700 1 $aBRAGANÇA, G. M. 700 1 $aBALARO, M. F. A. 700 1 $aARASHIRO, E. K. N. 700 1 $aSANTOS, G. B. 700 1 $aSOUZA, G. N. de 700 1 $aSOUZA-FABJAN, J. M. G. 700 1 $aFONSECA, J. F. da 700 1 $aBRANDÃO, F. Z. 773 $tReproduction in Domestic Animals$gv. 53, n. 1, p. 266-269, Feb. 2018.
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