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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
09/11/2017 |
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Data da última atualização: |
14/12/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FREITAS, J. P. X. de; DINIZ, R. P. A.; OLIVEIRA, S. A. S. de; SANTOS, V. da S.; OLIVEIRA, E. J. de. |
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Afiliação: |
JUAN PAULO XAVIER DE FREITAS, UFRB; RAFAEL PARREIRA DINIZ, UFRB; SAULO ALVES SANTOS DE OLIVEIRA, CNPMF; VANDERLEI DA SILVA SANTOS, CNPMF; EDER JORGE DE OLIVEIRA, CNPMF. |
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Título: |
Inbreeding depression for severity caused by leaf diseases in cassava. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Euphytica, p. 213-215, August, 2017. |
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ISSN: |
1573-5060 |
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DOI: |
10.1007/s10681-017-1995-0 |
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Idioma: |
Inglês |
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Conteúdo: |
he use of inbred populations in cassava has been little exploited by breeding programs. Therefore, the objective of this study was to evaluate the effects of inbreeding depression (ID) in S1 families for resistance to leaf diseases and to select transgressive individuals. Five cassava varieties (Cascuda, BRS Formosa, Fécula Branca, Mani-Branca and BRS Mulatinha) were selfed, and the families S0 and S1 were evaluated for severity caused by cassava white leaf spot (CWLS), cassava brown leaf spot (CBRLS) and cassava blight leaf spot (CBLS) under field conditions. Differences among S1 families and between generations (S0 × S1) were identified for all diseases. The wide variation of CWLS (2.16?93.38%), CBRLS (15.31?69.39%) and CBLS (15.70?53.64%) severity highlighted the occurrence of ID in S1 families, in which the average was 12.61% (CWLS), 13.72% (CBRLS) and 15.92% (CBLS). Mean estimates of homozygous loci contribution (µ + ?) were of higher magnitude compared with heterozygous loci contribution (?) for all diseases and families. Even in the occurrence of ID, it was possible to select resistant S1 individuals, in a way that the reduction in the severity of diseases based on the selection of the five most resistant individuals was 75.80% (S1-BRS Formosa) for CWLS, 38.64% (S1-Cascuda) for CBRLS and 33.51% (S1-Cascuda) for CBLS. These results demonstrate the predominance of additive genetic effects and the possibility to select transgressive individuals in S1 families for increasing the resistance to cassava leaf diseases. Menoshe use of inbred populations in cassava has been little exploited by breeding programs. Therefore, the objective of this study was to evaluate the effects of inbreeding depression (ID) in S1 families for resistance to leaf diseases and to select transgressive individuals. Five cassava varieties (Cascuda, BRS Formosa, Fécula Branca, Mani-Branca and BRS Mulatinha) were selfed, and the families S0 and S1 were evaluated for severity caused by cassava white leaf spot (CWLS), cassava brown leaf spot (CBRLS) and cassava blight leaf spot (CBLS) under field conditions. Differences among S1 families and between generations (S0 × S1) were identified for all diseases. The wide variation of CWLS (2.16?93.38%), CBRLS (15.31?69.39%) and CBLS (15.70?53.64%) severity highlighted the occurrence of ID in S1 families, in which the average was 12.61% (CWLS), 13.72% (CBRLS) and 15.92% (CBLS). Mean estimates of homozygous loci contribution (µ + ?) were of higher magnitude compared with heterozygous loci contribution (?) for all diseases and families. Even in the occurrence of ID, it was possible to select resistant S1 individuals, in a way that the reduction in the severity of diseases based on the selection of the five most resistant individuals was 75.80% (S1-BRS Formosa) for CWLS, 38.64% (S1-Cascuda) for CBRLS and 33.51% (S1-Cascuda) for CBLS. These results demonstrate the predominance of additive genetic effects and the possibility to select transgressive individuals in S1 families for increas... Mostrar Tudo |
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Thesagro: |
Mandioca. |
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Thesaurus Nal: |
Cassava. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02166naa a2200217 a 4500
001 2079401
005 2017-12-14
008 2017 bl uuuu u00u1 u #d
022 $a1573-5060
024 7 $a10.1007/s10681-017-1995-0$2DOI
100 1 $aFREITAS, J. P. X. de
245 $aInbreeding depression for severity caused by leaf diseases in cassava.$h[electronic resource]
260 $c2017
520 $ahe use of inbred populations in cassava has been little exploited by breeding programs. Therefore, the objective of this study was to evaluate the effects of inbreeding depression (ID) in S1 families for resistance to leaf diseases and to select transgressive individuals. Five cassava varieties (Cascuda, BRS Formosa, Fécula Branca, Mani-Branca and BRS Mulatinha) were selfed, and the families S0 and S1 were evaluated for severity caused by cassava white leaf spot (CWLS), cassava brown leaf spot (CBRLS) and cassava blight leaf spot (CBLS) under field conditions. Differences among S1 families and between generations (S0 × S1) were identified for all diseases. The wide variation of CWLS (2.16?93.38%), CBRLS (15.31?69.39%) and CBLS (15.70?53.64%) severity highlighted the occurrence of ID in S1 families, in which the average was 12.61% (CWLS), 13.72% (CBRLS) and 15.92% (CBLS). Mean estimates of homozygous loci contribution (µ + ?) were of higher magnitude compared with heterozygous loci contribution (?) for all diseases and families. Even in the occurrence of ID, it was possible to select resistant S1 individuals, in a way that the reduction in the severity of diseases based on the selection of the five most resistant individuals was 75.80% (S1-BRS Formosa) for CWLS, 38.64% (S1-Cascuda) for CBRLS and 33.51% (S1-Cascuda) for CBLS. These results demonstrate the predominance of additive genetic effects and the possibility to select transgressive individuals in S1 families for increasing the resistance to cassava leaf diseases.
650 $aCassava
650 $aMandioca
700 1 $aDINIZ, R. P. A.
700 1 $aOLIVEIRA, S. A. S. de
700 1 $aSANTOS, V. da S.
700 1 $aOLIVEIRA, E. J. de
773 $tEuphytica, p. 213-215, August, 2017.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
27/04/2011 |
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Data da última atualização: |
29/04/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ABREU, E. F. M.; ANDRADE, E. C. de; ARAGAO, F. J. L. |
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Afiliação: |
Emanuel Felipe Medereiros Abreu, UnB; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; FRANCISCO JOSE LIMA ARAGAO, CENARGEN. |
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Título: |
Molecular comparison between isolates of Cowpea severe mosaic virus and Cowpea aphid borne mosaic virus from Northeast Brazil. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM. |
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Idioma: |
Inglês |
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Notas: |
pdf 34699 |
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Conteúdo: |
Cowpea (Vigna unguiculata) is an important plant crop in Northeast Brazil being traditionally cultivated by small farmers. Virus diseases are considered to be the main factor limiting cowpea yield in the region. The severe mosaic disease caused by the Cowpea severe mosaic virus (CpSMV), family Comoviridae, genus Comovirus, seems to be one of the most prevalent diseases leading to high yield losses in this crop. The CABMV belongs to the genus Potyvirus in the Potyviridae family, and infects cowpea worldwide. In the northeastern Region of Brazil, both viruses can be found in cowpea planted areas. The aim of the present study was to access the degree of homology among a 2500 and 1500bp region of different isolates of CpSMV and CABMV, respectively; obtained in different northeastern regions in Brazil, and to compare it to isolates throughout the world. Plants with CPSMV and CABMV symptoms from the states of Piauí, Ceará, Rio Grande do Norte, Paraiba, Pernambuco, Alagoas, Sergipe and Bahia were collected, and the isolates were identified by RT-PCR analysis. Total RNA was extracted from infected tissue with Trizol Reagent (Invitrogen) according to manufacturer's recommendations, and afterwards used for synthesis of cDNA fragments by RT-PCR using the Superscript® III Reverse Polymerase and Taq DNA Polymerase (Invitrogen), according to manufacturer?s recommendations. The synthesized primers were able to amplify fragments of 2500 and 1500bp of the CpSMV and CABMV virus, respectively, by RT-PCR. Amplification products were directly cloned into the pGEMT-Easy plasmid vector (Promega), according to the manufacturer?s instructions. Cloned fragments were sequenced in both orientations. Deduced amino acid sequences of the virus were compared to sequences available from GenBank. Multiple sequence alignments were obtained with Clustal W. Phylogenetic trees using the MEGA version 4.1 software package and the neighbour-joining method with Poisson correction. Tree branches were bootstrapped with 1000 permutations. CpSMV and CABMV diseases remain as limiting factors in this crop in Brazil, and breeding programs, either by conventional or engineered approaches, should be targeted at establishing resistance of cowpeas to CpSMV and CABMV. MenosCowpea (Vigna unguiculata) is an important plant crop in Northeast Brazil being traditionally cultivated by small farmers. Virus diseases are considered to be the main factor limiting cowpea yield in the region. The severe mosaic disease caused by the Cowpea severe mosaic virus (CpSMV), family Comoviridae, genus Comovirus, seems to be one of the most prevalent diseases leading to high yield losses in this crop. The CABMV belongs to the genus Potyvirus in the Potyviridae family, and infects cowpea worldwide. In the northeastern Region of Brazil, both viruses can be found in cowpea planted areas. The aim of the present study was to access the degree of homology among a 2500 and 1500bp region of different isolates of CpSMV and CABMV, respectively; obtained in different northeastern regions in Brazil, and to compare it to isolates throughout the world. Plants with CPSMV and CABMV symptoms from the states of Piauí, Ceará, Rio Grande do Norte, Paraiba, Pernambuco, Alagoas, Sergipe and Bahia were collected, and the isolates were identified by RT-PCR analysis. Total RNA was extracted from infected tissue with Trizol Reagent (Invitrogen) according to manufacturer's recommendations, and afterwards used for synthesis of cDNA fragments by RT-PCR using the Superscript® III Reverse Polymerase and Taq DNA Polymerase (Invitrogen), according to manufacturer?s recommendations. The synthesized primers were able to amplify fragments of 2500 and 1500bp of the CpSMV and CABMV virus, respectively,... Mostrar Tudo |
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Palavras-Chave: |
CABMV; Cowpea; CpSMV; Molecular and RT-PCR analysis; Virus diseases. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02977nam a2200205 a 4500
001 1886945
005 2011-04-29
008 2011 bl uuuu u00u1 u #d
100 1 $aABREU, E. F. M.
245 $aMolecular comparison between isolates of Cowpea severe mosaic virus and Cowpea aphid borne mosaic virus from Northeast Brazil.
260 $aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. 1 CD-ROM.$c2011
500 $apdf 34699
520 $aCowpea (Vigna unguiculata) is an important plant crop in Northeast Brazil being traditionally cultivated by small farmers. Virus diseases are considered to be the main factor limiting cowpea yield in the region. The severe mosaic disease caused by the Cowpea severe mosaic virus (CpSMV), family Comoviridae, genus Comovirus, seems to be one of the most prevalent diseases leading to high yield losses in this crop. The CABMV belongs to the genus Potyvirus in the Potyviridae family, and infects cowpea worldwide. In the northeastern Region of Brazil, both viruses can be found in cowpea planted areas. The aim of the present study was to access the degree of homology among a 2500 and 1500bp region of different isolates of CpSMV and CABMV, respectively; obtained in different northeastern regions in Brazil, and to compare it to isolates throughout the world. Plants with CPSMV and CABMV symptoms from the states of Piauí, Ceará, Rio Grande do Norte, Paraiba, Pernambuco, Alagoas, Sergipe and Bahia were collected, and the isolates were identified by RT-PCR analysis. Total RNA was extracted from infected tissue with Trizol Reagent (Invitrogen) according to manufacturer's recommendations, and afterwards used for synthesis of cDNA fragments by RT-PCR using the Superscript® III Reverse Polymerase and Taq DNA Polymerase (Invitrogen), according to manufacturer?s recommendations. The synthesized primers were able to amplify fragments of 2500 and 1500bp of the CpSMV and CABMV virus, respectively, by RT-PCR. Amplification products were directly cloned into the pGEMT-Easy plasmid vector (Promega), according to the manufacturer?s instructions. Cloned fragments were sequenced in both orientations. Deduced amino acid sequences of the virus were compared to sequences available from GenBank. Multiple sequence alignments were obtained with Clustal W. Phylogenetic trees using the MEGA version 4.1 software package and the neighbour-joining method with Poisson correction. Tree branches were bootstrapped with 1000 permutations. CpSMV and CABMV diseases remain as limiting factors in this crop in Brazil, and breeding programs, either by conventional or engineered approaches, should be targeted at establishing resistance of cowpeas to CpSMV and CABMV.
653 $aCABMV
653 $aCowpea
653 $aCpSMV
653 $aMolecular and RT-PCR analysis
653 $aVirus diseases
700 1 $aANDRADE, E. C. de
700 1 $aARAGAO, F. J. L.
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