Data/hora: 04/06/2024 05:42:54

Biblioteca(s): 	Embrapa Mandioca e Fruticultura; Embrapa Recursos Genéticos e Biotecnologia.	
Data corrente: 	20/02/2013	
Data da última atualização: 	19/06/2023	
Autoria: 	ABREU, E. F. M.; TINOCO, M. L. P.; ANDRADE, E. C. de; ARAGAO, F. J. L.	
Afiliação: 	EMANUEL FELIPE MEDEIROS ABREU, CNPMF; MARIA LAINE PENHA TINOCO, CENARGEN; EDUARDO CHUMBINHO DE ANDRADE, SRI; FRANCISCO JOSE LIMA ARAGAO, CENARGEN.	
Título: 	Diversity among isolates of cowpea severe mosaic virus infecting cowpeas in northeastern Brazil.	
Ano de publicação: 	2012	
Fonte/Imprenta: 	Genetics and Molecular Research, v. 11, n. 3, p. 3146-3153, 2012.	
ISSN: 	1676-5680	
Idioma: 	Inglês	
Conteúdo: 	Eleven isolates of cowpea severe mosaic virus (CPSMV), a member of the genus Comovirus, were selected from 50 samples collected of nine cowpea fields in Northeastern Brazil (Piauí, Ceará, Rio Grande do Norte, Paraíba, Pernambuco, Alagoas, Sergipe, Bahia, and Distrito Federal) and partially sequenced. The RNA1 partial sequence, corresponding to the helicase, viral genome-linked protein, picornain 3C-like protease, and the RNA-directed RNA polymerase genes from CPSMV, had high identity among isolates, varying from 98 to 100%. No evidence was found for intermolecular or intramolecular recombination. Phylogenetic analysis confirmed that the Brazilian CPSMV isolates are substantially different from the CPSMV strain USA. Despite the low variability found among Brazilian CPSMV isolates, there were notable differences in the symptomatology of infected cowpea plants, ranging from mild to moderate. Previous reports have demonstrated an association between CPSMV symptom determinants and helicase. However, we found no correlation between the helicase Diversity among isolates of cowpea severe mosaic virus 3147 mutations and symptoms caused by CPSMV. Nevertheless, all isolates with mutation R to K in the protease provoked severe symptoms. This type of information can provide a foundation for the development of strategies to produce durable resistant cowpea lines. It is crucial for strategies based on DNA sequence-dependent technologies, such as inhibition with RNAi.	
Palavras-Chave: 	Isolate of cowpea.	
Thesaurus Nal: 	Cowpea severe mosaic virus.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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CENARGEN - (34375-1) - UPC - AP - DD - SP 20418 -  - SP 20418 - DIS