Data/hora: 22/06/2024 13:56:05

Biblioteca(s): | Embrapa Agricultura Digital; Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais.
Data corrente: | 30/11/2009
Data da última atualização: | 17/05/2022
Autoria: | PINHEIRO, P. V.; FARIA, J. C. de; NOGUEIRA, E. O. P. e L.; ARAGÃO, F. J. L.
Afiliação: | PATRICIA VALLE PINHEIRO, CNPAF; JOSIAS CORREA DE FARIA, CNPAF; ELSA OLIVEIRA P E LAGO NOGUEIRA, CENARGEN; FRANCISCO JOSE LIMA ARAGAO, CENARGEN.
Título: | Transgene inheritances and genetic similarities of near isogenic lines of genetically modified common beans.
Ano de publicação: | 2009
Fonte/Imprenta: | Pesquisa Agropecuária Brasileira, v. 44, n. 9, p. 1168-1176, set. 2009.
Idioma: | Inglês
Conteúdo: | The objective of the present work was to determine the inheritance and stability of transgenes of a transgenic bean line expressing the genes rep-trap-ren from Bean golden mosaic virus and the bar gene. Crosses were done between the transgenic line and four commercial bean cultivars, followed by four backcrosses to the commercial cultivars. Progenies from each cross were evaluated for the presence of the transgenes by brushing the leaves with glufosinate ammonium and by polymerase chain reaction using specifi c oligonucleotides. Advanced generations were rub-inoculated with an isolate of Bean common mosaic necrosis virus (BCMNV). The transgenes were inherited consistently in a Mendelian pattern in the four crosses studied. The analyzed lines recovered close to 80% of the characteristics of the recurrent parent, as determined by the random amplifi ed DNA markers used, besides maintaining important traits such as resistance to BCMNV. The presence of the transgene did not cause any detectable undesirable effect in the evaluated progenies.
Palavras-Chave: | BCMNV; Estabilidade genotípica do transgene; Genetic variability; Herança de transgenes de feijoeiro-comum geneticamente modificado; Variabilidade genética; Vírus do mosaico comum.
Thesagro: | Engenharia Genética; Feijão; Mosaico dourado; Phaseolus vulgaris; Virus.
Thesaurus Nal: | Genetic engineering; Phaseolus.

Categoria do assunto:

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X Pesquisa, Tecnologia e Engenharia

URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE-2010/46933/1/44n09a15.pdf
Registro original: | Embrapa Arroz e Feijão (CNPAF)

Exemplares:

CNPAF - (29425-1) - UPC - AP - DD - 2009 -  - 2009 - DIS
CNPTIA - (14252-1) - EMB - AP - PP -  -  -  - DIS
CENARGEN - (31968-1) - UPC - AP - PP - 630.5 -  -  - DIS
AI-SEDE - (46933-1) - UPE - AP - PP - 630.72081 - P474 -  - DIS