| |
|
|
| Registros recuperados : 60 | |
| 21. |  | FERREIRA, A. J.; ROJAS, J. D.; FAVARO, L. C. de L.; BRAGA, R. M.; ARAUJO, W. L. Desenvolvimento de ferramentas na análise de genes de Epicoccum nigrum. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 27., 2013, Natal, RN. Abstracts... São Paulo: Sociedade Brasileira de Microbiologia, 2013.| Biblioteca(s): Embrapa Agroenergia. |
|    |
| 22. | | DIAS, A. C. F.; ANDREOTE, F. D.; RIGONATO, J.; FIORE, M. F.; MELO, I. S. de; ARAUJO, W. L. The bacterial diversity in a Brazilian non-disturbed mangrove sediment. Antonie van Leeuwenhoek, v. 98, n. 4, p. 541-551, 2010.| Biblioteca(s): Embrapa Meio Ambiente. |
| |
| 23. | | CARAVIERI, F. A.; FERREIRA, A. J.; FERREIRA, A.; CLIVATI, D.; MIRANDA, V. F. O. de; ARAÚJO, W. L. Bacterial community associated with traps of the carnivorous plants Utricularia hydrocarpa and Genlisea filiformis Aquatic Botany, Amsterdam, v. 116, p. 8-12, 2014.| Biblioteca(s): Embrapa Agrossilvipastoril. |
|  |
| 25. | | TORRES, A. R.; ARAÚJO, W. L.; CURSINO, L.; ROSSETTO, P. de B.; MONDIN, M.; HUNGRIA, M.; AZEVEDO, J. L. Colonization of Madagascar periwinkle (Catharanthus roseus), by endophytes encoding gfp marker. Archives of Microbiology, Berlin, v. 195, n. 7, p. 483-489, Jul. 2013.| Biblioteca(s): Embrapa Soja. |
| |
| 26. | | Quecine, M. C.; Araújo, W. L.; Rosseto, P. B.; FERREIRA, A.; TSUI, S.; Lacava, P. T.; Mondin, M.; Azevedo, J. L.; Pizzirani-Kleiner, A. A. Sugarcane growth promotion by the endophytic bacterium Pantoea agglomerans 33.1. Applied and Environmental Microbiology, v. 78, n. 21, p. 7511-7518, 2012.| Biblioteca(s): Embrapa Agrossilvipastoril. |
| |
| 27. | | MARTINS, P. C.; CAMPOS, M. M.; JAYME, C. G.; MACHADO, F. S.; TOMICH, T. R.; PEREIRA, L. G. R.; SILVA, R. B. e; ARAÚJO, W. L. de P. Levantamento das técnicas zootécnicas utilizadas na criação de bezerras leiteiras no município de Rio Pomba, MG. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 15., 2015, Juiz de Fora, MG. Anais... Juiz de Fora: Embrapa Gado de Leite, 2015. (Embrapa Gado de Leite. Documentos, 176.). 1 p.| Biblioteca(s): Embrapa Gado de Leite. |
| |
| 28. | | DIAS, A. C. F.; ANDREOTE, F. D.; DINI-ANDREOTE, F.; LACAVA, P. T.; SÁ, A. L. B.; MELO, I. S. de; AZEVEDO, J. L.; ARAUJO, W. L. Diversity and biotechnological potential of culturable bacteria from Brazilian mangrove sediment. World Journal of Microbiology and Biotechnology, v. 25, n. 7, p. 1305-1311, 2009.| Biblioteca(s): Embrapa Meio Ambiente. |
| |
| 29. | | LACAVA, P. T.; SILVA-STENICO, M. E.; ARAÚJO, W. L.; SIMIONATO, A. V. C.; CARRILHO, E.; TSAI, S. M.; AZEVEDO, J. L. Detection of siderophores in endophytic bacteria Methylobacterium spp. associated with Xylella fastidiosa subsp. pauca. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 43, n. 4, p. 521-528, abr. 2008 Título em português: Detecção de sideróforos nas bactérias endofíticas Methylobacterium spp. associadas com Xylella fastidiosa subsp. pauca.| Biblioteca(s): Embrapa Unidades Centrais. |
| |
| 30. | | BATISTA, K. D.; ARAÚJO, W. L.; ANTUNES, W. C.; CAVATTE, P. C.; MORAES, G. A. B. K.; MARTINS, S. C. V.; DAMATTA, F. M. Photosynthetic limitations in coffee plants are chiefly governed by diffusive factors. Trees, v. 26, p. 459-468, 2012.| Biblioteca(s): Embrapa Roraima. |
| |
| 31. | | FAVARO, L. C. de L.; MELO, F. L.; ARAÚJO, F. D. S.; MARSAIOLI, A. J.; SILVA, M. A.; ROJAS, R. D.; ARAÚJO, W. L. Rational bioprospecting and identi cation of genes from sugarcane endophytic fungi Epicoccum nigrum and Epicoccum sp. involved in the biosynthesis of bioactive metabolites. In: REUNIÃO DE GENÉTICA DE MICRORGANISMOS - REGEM, 28., 2012, Foz do Iguaçu, PR. [Anais...]. Ribeirão Preto: Sociedade Brasileira de Genética, 2012. Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
| |
| 32. | | RODRIGUES, M. B. C.; ANDREOTE, F. D.; SPÓSITO, M. B.; AGUILLAR-VILDOSO, C. I.; ARAÚJO, W. L.; PIZZIRANI-KLEINER, A.A. Resistência a benzimidazóis por Guignardia citricarpa. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 42, n. 3, p. 323-327, mar. 2007 Título em inglês: Guignardia citricarpa resistance to benzimidazoles.| Biblioteca(s): Embrapa Unidades Centrais. |
| |
| 33. | | SOUSA, G. P.; JARAMILLO, P. M. D.; MENDES, T. D.; SALUM, T. F. C.; ARAÚJO, W. L.; FAVARO, L. C. de L. Prospecção de fungos endofíticos de cana-de-açúcar para produção de enzimas lignocelulolítcas. In: SIMPÓSIO DE RECURSOS GENÉTICOS PARA A AMÉRICA LATINA E CARIBE, 10., 2015, Bento Gonçalves. Recursos genéticos no século 21: de Vavilov a Svalbard. Anais... [s.l.]: Sociedade Brasileira de Recursos Genéticos, 2015.| Biblioteca(s): Embrapa Agroenergia. |
| |
| 34. | | GOMES, T. C.; CONRADO, R.; OLIVEIRA, R. C. de; SELARI, P. J. R. G.; MELO, I. S. de; ARAÚJO, W. L.; MARIA, D. A.; SOUZA, A. O. de. Effect of Monocerin, a fungal secondary metabolite, on endothelial cells. Toxins v. 15, n. 5, article 344, 2023.| Biblioteca(s): Embrapa Meio Ambiente. |
| |
| 35. | | SIQUEIRA, J. A.; BARROS, J. A. S.; DAL-BIANCO, M.; MARTINS, S. C. V.; MAGALHAES, P. C.; DAMATTA, F. M.; ARAÚJO, W. L.; RIBEIRO, C. Metabolic and physiological adjustments of maize leaves in response to aluminum stress. Theoretical and Experimental Plant Physiology, v. 32, n. 2, p. 133-145, 2020.| Biblioteca(s): Embrapa Milho e Sorgo. |
| |
| 36. | | CHAPPER, M.; LOUREIRO, M. E.; MOSQUIM, P. R.; ARAÚJO, W. L.; PEREIRA, A. da S.; FINGER, F. L.; SIMÕES, A. N. Mudanças metabólicas após recondicionamento a 15 C de tubérculos de batata armazenados a baixa temperatura. Horticultura Brasileira, Brasília, DF, v. 22, n. 4, p. 700-705, out./dez. 2006.| Biblioteca(s): Embrapa Hortaliças. |
| |
| 37. | | FAVARO, L. C. de L.; SEBASTINES, F. L. S.; LACAVA, P. T.; RODRIGUES, M. B. C.; ARAÚJO, W. L.; AZEVEDO, J. L.; KLEINER, A. A. P. Genetic transformation of Diaporthe phaseolorum, an endophytic fungus found in mangrove forests, mediated by Agrobacterium tumefaciens. Current Genetics, v. 58, p. 21?33, 2012. 21-33| Biblioteca(s): Embrapa Agroenergia. |
| |
| 38. | | SEBASTIANES, F. L. S.; LACAVA, P. T.; FAVARO, L. C. de L.; RODRIGUES, M. B. C.; ARAÚJO, W. L.; AZEVEDO, J. L.; PIZZIRANI-KLEINER, A. A. Genetic transformation of Diaporthe phaseolorum, an endophytic fungus found in mangrove forests, mediated by Agrobacterium tumefaciens. Current Genetics, Dec. 2011. Não paginado. DOI 10.1007/s00294-011-0362-2.| Biblioteca(s): Embrapa Agroenergia. |
| |
| 39. | | FERREIRA, A. J.; OLIVEIRA, L. S.; ALVES, J. M. P.; THON, M.; DURHAM, A. M.; FAVARO, L. C. de L.; GRUBER, A.; ARAÚJO, W. L. Genome analysis of E. nigrum and other filamentous fungi reveals molecular mechanisms related to endophytic/pathogenic lifestyles. In: X-MEETING; INTERNATIONAL CONFERENCE OF the AB3C, 2016, Belo Horizonte, MG. [Anais ... ]. Ribeirão Preto: AB3C, 2016. Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
| |
| 40. | | SOUSA, G. P.; MENDES, T. D.; FRANCO, P. F.; ARAÚJO, W. L.; RODRIGUES, D. de S.; SALUM, T. F. C.; FAVARO, L. C. de L. Fungos endofíticos de cana-de-açúcar e seu potencial para produção de enzimas lignocelulolíticas. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 3., 2014, Santos, SP. [Anais ...]. Brasília, DF: SBRG, 2014. Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
| |
| Registros recuperados : 60 | |
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Agrossilvipastoril. |
|
Data corrente: |
03/02/2014 |
|
Data da última atualização: |
10/08/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 1 |
|
Autoria: |
RODRIGUES, M. B. C.; FAVARO, L. C. de L.; PALLU, A. P. de S.; FERREIRA, A.; SEBASTIANES, F. de S.; RODRIGUES, M. J. C.; SPÓSITO, M. B.; ARAÚJO, W. L. de; PIZZIRANI-KLEINER, A. A. |
|
Afiliação: |
Maria Beatriz Calderan Rodrigues, USP-ESALQ; LEIA CECILIA DE LIMA FAVARO, CNPAE; Ana Paula de Souza Pallu, USP-ESALQ; ANDERSON FERREIRA, CPAMT; Fernanda de Souza Sebastianes, USP-ESALQ; Maria Juliana Calderan Rodrigues, USP-ESALQ; Marcel Bellato Spósito, USP-ESALQ; Welington Luiz DE Araújo, UMC; Aline Aparecida Pizzirani-Kleiner, USP-ESALQ. |
|
Título: |
Agrobacterium-mediated transformation of Guignardia citricarpa: an efficient tool to gene transfer and random mutagenesis. |
|
Ano de publicação: |
2013 |
|
Fonte/Imprenta: |
Fungal Biology, Oxford, v. 117, n. 7/8, p. 556-568, jul./aug., 2013. |
|
ISSN: |
1878-6146 |
|
DOI: |
http://dx.doi.org/10.1016/j.funbio.2013.06.005 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Guignardia citricarpa is the causal agent of Citrus Black Spot (CBS), an important disease in Citriculture. Due to the expressive value of this activity worldwide, especially in Brazil, understanding more about the functioning of this fungus is of utmost relevance, making possible the elucidation of its infection mechanisms, and providing tools to control CBS. This work describes for the first time an efficient and successful methodology for genetic transformation of G. citricarpa mycelia, which generated transformants expressing the gene encoding for the gfp (green fluorescent protein) and also their interaction with citrus plant. Mycelia of G. citricarpa were transformed via Agrobacterium tumefaciens, which carried the plasmid pFAT-gfp, contains the genes for hygromycin resistance (hph) as well as gfp. The optimization of the agrotransformation protocol was performed testing different conditions (type of membrane; inductor agent concentration [acetosyringonee - AS] and cocultivation time). Results demonstrated that the best condition occurred with the utilization of cellulose?s ester membrane; 200µM of AS and 96 h as cocultivation time. High mitotic stability (82 %) was displayed by transformants using Polymerase Chain Reaction (PCR) technique to confirm the hph gene insertion. In addition, the presence of gfp was observed inside mycelia by epifluorescence optical microscopy. This technique easy visualization of the behaviour of the pathogen interacting with the plant for the first time, allowing future studies on the pathogenesis of this fungus. The establishment of a transformation method for G. citricarpa opens a range of possibilities and facilitates the study of insertional mutagenesis and genetic knockouts, in order to identify the most important genes involved in the pathogenesis mechanisms and plant-pathogen interaction. MenosGuignardia citricarpa is the causal agent of Citrus Black Spot (CBS), an important disease in Citriculture. Due to the expressive value of this activity worldwide, especially in Brazil, understanding more about the functioning of this fungus is of utmost relevance, making possible the elucidation of its infection mechanisms, and providing tools to control CBS. This work describes for the first time an efficient and successful methodology for genetic transformation of G. citricarpa mycelia, which generated transformants expressing the gene encoding for the gfp (green fluorescent protein) and also their interaction with citrus plant. Mycelia of G. citricarpa were transformed via Agrobacterium tumefaciens, which carried the plasmid pFAT-gfp, contains the genes for hygromycin resistance (hph) as well as gfp. The optimization of the agrotransformation protocol was performed testing different conditions (type of membrane; inductor agent concentration [acetosyringonee - AS] and cocultivation time). Results demonstrated that the best condition occurred with the utilization of cellulose?s ester membrane; 200µM of AS and 96 h as cocultivation time. High mitotic stability (82 %) was displayed by transformants using Polymerase Chain Reaction (PCR) technique to confirm the hph gene insertion. In addition, the presence of gfp was observed inside mycelia by epifluorescence optical microscopy. This technique easy visualization of the behaviour of the pathogen interacting with the plant for ... Mostrar Tudo |
|
Palavras-Chave: |
Agrobacterium-Mediated; Plant-Pathogen Interaction. |
|
Thesaurus NAL: |
citrus black spot; genetic transformation; Guignardia citricarpa. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/96268/1/cpamt-Ferreira-1878-6146-2013.pdf
|
|
Marc: |
LEADER 02874naa a2200301 a 4500
001 1978312
005 2017-08-10
008 2013 bl uuuu u00u1 u #d
022 $a1878-6146
024 7 $ahttp://dx.doi.org/10.1016/j.funbio.2013.06.005$2DOI
100 1 $aRODRIGUES, M. B. C.
245 $aAgrobacterium-mediated transformation of Guignardia citricarpa$ban efficient tool to gene transfer and random mutagenesis.$h[electronic resource]
260 $c2013
520 $aGuignardia citricarpa is the causal agent of Citrus Black Spot (CBS), an important disease in Citriculture. Due to the expressive value of this activity worldwide, especially in Brazil, understanding more about the functioning of this fungus is of utmost relevance, making possible the elucidation of its infection mechanisms, and providing tools to control CBS. This work describes for the first time an efficient and successful methodology for genetic transformation of G. citricarpa mycelia, which generated transformants expressing the gene encoding for the gfp (green fluorescent protein) and also their interaction with citrus plant. Mycelia of G. citricarpa were transformed via Agrobacterium tumefaciens, which carried the plasmid pFAT-gfp, contains the genes for hygromycin resistance (hph) as well as gfp. The optimization of the agrotransformation protocol was performed testing different conditions (type of membrane; inductor agent concentration [acetosyringonee - AS] and cocultivation time). Results demonstrated that the best condition occurred with the utilization of cellulose?s ester membrane; 200µM of AS and 96 h as cocultivation time. High mitotic stability (82 %) was displayed by transformants using Polymerase Chain Reaction (PCR) technique to confirm the hph gene insertion. In addition, the presence of gfp was observed inside mycelia by epifluorescence optical microscopy. This technique easy visualization of the behaviour of the pathogen interacting with the plant for the first time, allowing future studies on the pathogenesis of this fungus. The establishment of a transformation method for G. citricarpa opens a range of possibilities and facilitates the study of insertional mutagenesis and genetic knockouts, in order to identify the most important genes involved in the pathogenesis mechanisms and plant-pathogen interaction.
650 $acitrus black spot
650 $agenetic transformation
650 $aGuignardia citricarpa
653 $aAgrobacterium-Mediated
653 $aPlant-Pathogen Interaction
700 1 $aFAVARO, L. C. de L.
700 1 $aPALLU, A. P. de S.
700 1 $aFERREIRA, A.
700 1 $aSEBASTIANES, F. de S.
700 1 $aRODRIGUES, M. J. C.
700 1 $aSPÓSITO, M. B.
700 1 $aARAÚJO, W. L. de
700 1 $aPIZZIRANI-KLEINER, A. A.
773 $tFungal Biology, Oxford$gv. 117, n. 7/8, p. 556-568, jul./aug., 2013.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agrossilvipastoril (CPAMT) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
