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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, C. S.; SERAPIÃO, R. V.; CAMARGO, A. J. DOS R.; FREITAS, C. de; IGUMA, L. T.; CARVALHO, B. C. de; CAMARGO, L. S. de A.; OLIVEIRA, L. Z.; VERNEQUE, R. da S. |
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Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; Raquel Varella Serapião; Agostinho Jorge dos Reis Camargo; CELIO DE FREITAS, CNPGL; Lilian Tamy Iguma; BRUNO CAMPOS DE CARVALHO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Letícia Zoccolaro Oliveira; RUI DA SILVA VERNEQUE, CNPGL. |
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Título: |
Oocyte origin affects the in vitro embryo production and development of Holstein (Bos taurus taurus) - Gyr (Bos taurus indicus) reciprocal cross embryos. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Animal Reproduction Science, v. 209, article 106165, 2019. |
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DOI: |
https://doi.org/10.1016/j.anireprosci.2019.106165 |
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Idioma: |
Inglês |
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Conteúdo: |
A reciprocal crossbred embryo production approach was used to assess effects of maternal breed on embryo development in tropical conditions (average temperature 22.0 °C and 77.9% relative humidity). Oocytes were recovered by ovum pick-up (OPU) from Gyr and Holstein donors (n = 90 Holstein and 83 Gyr OPUs). Female F1 embryos were produced by fertilization with sperm bearing X-chromosomes from Holstein semen (n = 615 Gyr oocytes) or Gyr semen (n = 255 Holstein oocytes). Blastocysts were transferred to recipients 168 h post-insemination (h.p.i.) (n= 70?144) and there were assessments of pregnancies until birth. Oocyte number per OPU (Gyr 10.0 ± 0.7 compared with Holstein 6.3 ± 0.4) and percentage viable oocytes (Gyr 78.8 ± 1.9% compared with Holstein 71.2 ± 2.2%) were less for Holstein donor animals. There was a 2.8 fold fewer total number of F1 blastocysts when Holstein donors were used (Gyr: 260, Holstein: 91). Pregnancy assessment during the different stages of gestation indicated the percentage pregnancy was less when embryos were produced from Holstein oocytes (Gyr and Holstein respectively: early pregnancy, 47.9% compared with 38.6%; mid-pregnancy, 44.4% compared with 31.4%; late pregnancy, 41.0% compared with 22.9%). Pregnancy length was also affected by maternal breed (Gyr: 280.8 ± 0.6, Holstein: 286.3 ± 0.7). It is concluded that in a tropical environment the maternal breed affects crossbred embryo development with pregnancy rates during the latter stages of gestation being greater when Gyr oocytes are used for production of embryos. MenosA reciprocal crossbred embryo production approach was used to assess effects of maternal breed on embryo development in tropical conditions (average temperature 22.0 °C and 77.9% relative humidity). Oocytes were recovered by ovum pick-up (OPU) from Gyr and Holstein donors (n = 90 Holstein and 83 Gyr OPUs). Female F1 embryos were produced by fertilization with sperm bearing X-chromosomes from Holstein semen (n = 615 Gyr oocytes) or Gyr semen (n = 255 Holstein oocytes). Blastocysts were transferred to recipients 168 h post-insemination (h.p.i.) (n= 70?144) and there were assessments of pregnancies until birth. Oocyte number per OPU (Gyr 10.0 ± 0.7 compared with Holstein 6.3 ± 0.4) and percentage viable oocytes (Gyr 78.8 ± 1.9% compared with Holstein 71.2 ± 2.2%) were less for Holstein donor animals. There was a 2.8 fold fewer total number of F1 blastocysts when Holstein donors were used (Gyr: 260, Holstein: 91). Pregnancy assessment during the different stages of gestation indicated the percentage pregnancy was less when embryos were produced from Holstein oocytes (Gyr and Holstein respectively: early pregnancy, 47.9% compared with 38.6%; mid-pregnancy, 44.4% compared with 31.4%; late pregnancy, 41.0% compared with 22.9%). Pregnancy length was also affected by maternal breed (Gyr: 280.8 ± 0.6, Holstein: 286.3 ± 0.7). It is concluded that in a tropical environment the maternal breed affects crossbred embryo development with pregnancy rates during the latter stages of gestation ... Mostrar Tudo |
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Palavras-Chave: |
Maternal breed; Oocyte quality. |
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Thesaurus Nal: |
Crossbreds; Gir (cattle breed); Girolando. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02523naa a2200289 a 4500
001 2118697
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.anireprosci.2019.106165$2DOI
100 1 $aOLIVEIRA, C. S.
245 $aOocyte origin affects the in vitro embryo production and development of Holstein (Bos taurus taurus) - Gyr (Bos taurus indicus) reciprocal cross embryos.$h[electronic resource]
260 $c2019
520 $aA reciprocal crossbred embryo production approach was used to assess effects of maternal breed on embryo development in tropical conditions (average temperature 22.0 °C and 77.9% relative humidity). Oocytes were recovered by ovum pick-up (OPU) from Gyr and Holstein donors (n = 90 Holstein and 83 Gyr OPUs). Female F1 embryos were produced by fertilization with sperm bearing X-chromosomes from Holstein semen (n = 615 Gyr oocytes) or Gyr semen (n = 255 Holstein oocytes). Blastocysts were transferred to recipients 168 h post-insemination (h.p.i.) (n= 70?144) and there were assessments of pregnancies until birth. Oocyte number per OPU (Gyr 10.0 ± 0.7 compared with Holstein 6.3 ± 0.4) and percentage viable oocytes (Gyr 78.8 ± 1.9% compared with Holstein 71.2 ± 2.2%) were less for Holstein donor animals. There was a 2.8 fold fewer total number of F1 blastocysts when Holstein donors were used (Gyr: 260, Holstein: 91). Pregnancy assessment during the different stages of gestation indicated the percentage pregnancy was less when embryos were produced from Holstein oocytes (Gyr and Holstein respectively: early pregnancy, 47.9% compared with 38.6%; mid-pregnancy, 44.4% compared with 31.4%; late pregnancy, 41.0% compared with 22.9%). Pregnancy length was also affected by maternal breed (Gyr: 280.8 ± 0.6, Holstein: 286.3 ± 0.7). It is concluded that in a tropical environment the maternal breed affects crossbred embryo development with pregnancy rates during the latter stages of gestation being greater when Gyr oocytes are used for production of embryos.
650 $aCrossbreds
650 $aGir (cattle breed)
650 $aGirolando
653 $aMaternal breed
653 $aOocyte quality
700 1 $aSERAPIÃO, R. V.
700 1 $aCAMARGO, A. J. DOS R.
700 1 $aFREITAS, C. de
700 1 $aIGUMA, L. T.
700 1 $aCARVALHO, B. C. de
700 1 $aCAMARGO, L. S. de A.
700 1 $aOLIVEIRA, L. Z.
700 1 $aVERNEQUE, R. da S.
773 $tAnimal Reproduction Science$gv. 209, article 106165, 2019.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Roraima. |
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Data corrente: |
28/01/2022 |
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Data da última atualização: |
28/01/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ARAÚJO, M. da C. da R.; CHAGAS, E. A.; VENDRAME, W.; RIBEIRO, M. I. G.; MOURA, E. A. de; TAVEIRA, D. L. L.; CHAGAS, P. C.; GRIGIO, M. L. |
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Afiliação: |
EDVAN ALVES CHAGAS, CPAF-RR. |
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Título: |
Callus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, v. 21, N. 3, 2021. |
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Idioma: |
Inglês |
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Conteúdo: |
Caçari is a fruit tree that is native to the Amazon region of Brazil. The fruit is considered to have the highest vitamin C content of any edible fruit (13.757 mg 100 g-1 dry weight). The objective of this study was to evaluate the effects of 2,4-D and 6-BAP alone or in combination on in vitro callus induction and pro-embryogenic mass formation in caçari stem segments in different culture media as a precursor for caçari micropropagation. The experiment consisted of two culture media (MS and WPM) and combinations of four concentrations of 2,4-D (0, 1, 2 and 4 mg L-1) and BAP (0, 0.25, 0.5 and 1 mg L-1). The results showed that the highest percentage of callus formation (99%) was obtained in WPM supplemented with 4 mg L-1, 2,4-D and 1 mg L-1 BAP, and this combination resulted in 93% pro-embryogenic mass formation. |
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Palavras-Chave: |
Camu-camu. |
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Thesaurus NAL: |
Growth regulators; Somatic embryogenesis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/230705/1/DocPlayer.pdf
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Marc: |
LEADER 01576naa a2200241 a 4500
001 2139460
005 2022-01-28
008 2021 bl uuuu u00u1 u #d
100 1 $aARAÚJO, M. da C. da R.
245 $aCallus induction and pro-embryogenic mass formation in Myrciaria dubia, an important medicinal and nutritional plant.$h[electronic resource]
260 $c2021
520 $aCaçari is a fruit tree that is native to the Amazon region of Brazil. The fruit is considered to have the highest vitamin C content of any edible fruit (13.757 mg 100 g-1 dry weight). The objective of this study was to evaluate the effects of 2,4-D and 6-BAP alone or in combination on in vitro callus induction and pro-embryogenic mass formation in caçari stem segments in different culture media as a precursor for caçari micropropagation. The experiment consisted of two culture media (MS and WPM) and combinations of four concentrations of 2,4-D (0, 1, 2 and 4 mg L-1) and BAP (0, 0.25, 0.5 and 1 mg L-1). The results showed that the highest percentage of callus formation (99%) was obtained in WPM supplemented with 4 mg L-1, 2,4-D and 1 mg L-1 BAP, and this combination resulted in 93% pro-embryogenic mass formation.
650 $aGrowth regulators
650 $aSomatic embryogenesis
653 $aCamu-camu
700 1 $aCHAGAS, E. A.
700 1 $aVENDRAME, W.
700 1 $aRIBEIRO, M. I. G.
700 1 $aMOURA, E. A. de
700 1 $aTAVEIRA, D. L. L.
700 1 $aCHAGAS, P. C.
700 1 $aGRIGIO, M. L.
773 $tCrop Breeding and Applied Biotechnology$gv. 21, N. 3, 2021.
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Registro original: |
Embrapa Roraima (CPAF-RR) |
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