Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
24/05/2021 |
Data da última atualização: |
24/05/2021 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
PRADO, G. S.; PINHEIRO, T. T.; FARIA, J. C. de; VIANELLO, R. P. |
Afiliação: |
GUILHERME SOUZA PRADO, especialista visitante CNPAF; THAÍSA TESSUTTI PINHEIRO, especialista visitante CNPAF; JOSIAS CORREA DE FARIA, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. |
Título: |
Genome editing via non-homologous end-joining (NHEJ) and ribonucleoproteins (RNP). |
Título original: |
Tecnologia CRISPR na edição genômica de plantas: biotecnologia aplicada à agricultura, 2020. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
In: MOLINARI, H. B. C.; VIEIRA, L. R.; SILVA, N. V. e; PRADO, G. S.; LOPES FILHO, J. F. (Ed.). CRISPR technology in plant genome editing: biotechnology applied to agriculture. Brasília, DF : Embrapa, 2021. |
Páginas: |
p. 47-88. |
Descrição Física: |
il. |
ISBN: |
978-65-87380-10-0 |
Idioma: |
Inglês |
Conteúdo: |
We will focus here on the use of the CRISPR/Cas system to induce mutations via NHEJ. The NHEJ mechanism acts in most of the cell cycle and is not based on homology to repair DNA, causing mutations such as insertions or deletions (indels) or even substitutions. In this way, the expression of the genes in the place where the repair occurred by NHEJ can, in general, be interrupted, and the encoded proteins may not be functional. To clarify the process of genome editing by CRISPR, this chapter aims to address the details of the NHEJ mechanism, as well as the use of ribonucleoprotein systems (RNPs) to promote editing, in addition to providing a practical procedure for the design of experiments. |
Palavras-Chave: |
CRISPR; CRISPRevolution; Non-homologous. |
Thesagro: |
Biotecnologia; DNA; Engenharia Genética; Genoma; RNA. |
Thesaurus Nal: |
Biotechnology; Genetic engineering; Nucleases; Plant genetics; Ribonucleoproteins; RNA editing. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/223400/1/chap2.pdf
|
Marc: |
LEADER 01908naa a2200361 a 4500 001 2131989 005 2021-05-24 008 2021 bl uuuu u00u1 u #d 020 $a978-65-87380-10-0 100 1 $aPRADO, G. S. 240 $aTecnologia CRISPR na edição genômica de plantas: biotecnologia aplicada à agricultura, 2020. 245 $aGenome editing via non-homologous end-joining (NHEJ) and ribonucleoproteins (RNP).$h[electronic resource] 260 $c2021 300 $ap. 47-88.$cil. 520 $aWe will focus here on the use of the CRISPR/Cas system to induce mutations via NHEJ. The NHEJ mechanism acts in most of the cell cycle and is not based on homology to repair DNA, causing mutations such as insertions or deletions (indels) or even substitutions. In this way, the expression of the genes in the place where the repair occurred by NHEJ can, in general, be interrupted, and the encoded proteins may not be functional. To clarify the process of genome editing by CRISPR, this chapter aims to address the details of the NHEJ mechanism, as well as the use of ribonucleoprotein systems (RNPs) to promote editing, in addition to providing a practical procedure for the design of experiments. 650 $aBiotechnology 650 $aGenetic engineering 650 $aNucleases 650 $aPlant genetics 650 $aRibonucleoproteins 650 $aRNA editing 650 $aBiotecnologia 650 $aDNA 650 $aEngenharia Genética 650 $aGenoma 650 $aRNA 653 $aCRISPR 653 $aCRISPRevolution 653 $aNon-homologous 700 1 $aPINHEIRO, T. T. 700 1 $aFARIA, J. C. de 700 1 $aVIANELLO, R. P. 773 $tIn: MOLINARI, H. B. C.; VIEIRA, L. R.; SILVA, N. V. e; PRADO, G. S.; LOPES FILHO, J. F. (Ed.). CRISPR technology in plant genome editing: biotechnology applied to agriculture. Brasília, DF : Embrapa, 2021.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|