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Registro Completo |
Biblioteca(s): |
Embrapa Amapá. |
Data corrente: |
16/02/2017 |
Data da última atualização: |
09/03/2017 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
ROCHA, M. de M.; SILVA, K. J. D. e; MENEZES JUNIOR, J. A. de; OLIVEIRA, I. J. de; RODRIGUES, J. E. L. F.; FREIRE FILHO, F. R.; MARINHO, J. T. de S.; CAVALCANTE, E. da S.; VILARINHO, A. A.; VIEIRA JUNIOR, J. R.; NASCIMENTO, I. R. do. |
Afiliação: |
MAURISRAEL DE MOURA ROCHA, CPAMN; KAESEL JACKSON DAMASCENO E SILVA, CPAMN; JOSE ANGELO NOGUEIRA DE M JUNIOR, CPAMN; INOCENCIO JUNIOR DE OLIVEIRA, CPAA; JOAO ELIAS LOPES F RODRIGUES, CPATU; FRANCISCO RODRIGUES FREIRE FILHO, CPATU; JOSE TADEU DE SOUZA MARINHO, CPAF-Acre; EMANUEL DA SILVA CAVALCANTE, CPAF-AP; ALOISIO ALCANTARA VILARINHO, CPAF-Roraima; JOSE ROBERTO VIEIRA JUNIOR, CPAF-Rondonia; Ildon Rodrigues do Nascimento, UFT. |
Título: |
Adaptabilidade e estabilidade produtiva de genótipos de feijão-caupi semieretos na região Norte do Brasil via procedimento REML/BLUP. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 4., 2016, Sorriso. Feijão-caupi: avanços e desafios tecnológicos e de mercado: resumos. Brasília, DF: Embrapa, 2016. |
Páginas: |
p. 125. |
Idioma: |
Português |
Palavras-Chave: |
Feijão-caupi; Interação genótipo x ambiente. |
Thesagro: |
Produtividade; Vigna Unguiculata. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 01062naa a2200289 a 4500 001 2064537 005 2017-03-09 008 2016 bl uuuu u00u1 u #d 100 1 $aROCHA, M. de M. 245 $aAdaptabilidade e estabilidade produtiva de genótipos de feijão-caupi semieretos na região Norte do Brasil via procedimento REML/BLUP.$h[electronic resource] 260 $c2016 300 $ap. 125. 650 $aProdutividade 650 $aVigna Unguiculata 653 $aFeijão-caupi 653 $aInteração genótipo x ambiente 700 1 $aSILVA, K. J. D. e 700 1 $aMENEZES JUNIOR, J. A. de 700 1 $aOLIVEIRA, I. J. de 700 1 $aRODRIGUES, J. E. L. F. 700 1 $aFREIRE FILHO, F. R. 700 1 $aMARINHO, J. T. de S. 700 1 $aCAVALCANTE, E. da S. 700 1 $aVILARINHO, A. A. 700 1 $aVIEIRA JUNIOR, J. R. 700 1 $aNASCIMENTO, I. R. do 773 $tIn: CONGRESSO NACIONAL DE FEIJÃO-CAUPI, 4., 2016, Sorriso. Feijão-caupi: avanços e desafios tecnológicos e de mercado: resumos. Brasília, DF: Embrapa, 2016.
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Embrapa Amapá (CPAF-AP) |
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Registro Completo
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Caprinos e Ovinos. |
Data corrente: |
27/09/2019 |
Data da última atualização: |
07/11/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
AZEVEDO, D. A. A. de; MONTEIRO, J. P.; PINHEIRO, R. R.; MUDADU, M. de A.; ANDRIOLI, A.; ARAÚJO, J. F.; SOUSA, A. L. M. de; SIDER, L. H.; PEIXOTO, R. M.; TEIXEIRA, M. F. da S. |
Afiliação: |
DALVA ALANA ARAGÃO DE AZEVEDO, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil; JOMAR PATRICIO MONTEIRO, CNPC; RAYMUNDO RIZALDO PINHEIRO, CNPC; MAURICIO DE ALVARENGA MUDADU, CNPTIA; ALICE ANDRIOLI, CNPC; JUSCILÂNIA FURTADO ARAÚJO, Northeast Biotechnology Network, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil; ANA LÍDIA MADEIRA DE SOUSA, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil; LUCIA HELENA SIDER, CNPC; RENATO MESQUITA PEIXOTO, Universidade Federal do Acre (UFAC) - Rio Branco, AC, Brazil; MARIA FÁTIMA DA SILVA TEIXEIRA, Universidade Estadual do Ceará (UECE) - Fortaleza, CE, Brazil. |
Título: |
Molecular characterization of circulating strains of small ruminant lentiviruses in Brazil based on complete gag and pol genes. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Small Ruminant Research, v. 177, p. 160-166, 2019. |
DOI: |
https://doi.org/10.1016/j.smallrumres.2019.06.011 |
Idioma: |
Inglês |
Conteúdo: |
Abstract: Small ruminant lentiviruses (SRLV) are widely distributed in the world and cause productive and economic losses. These pathogens have high genetic variability, which hinders diagnosis by molecular methods. Molecular characterization studies are good strategies for elaborating programs aimed to control or eradicate diseases. Hence, this study aimed to sequence, characterize and compare strains of SRLV, which were isolated from naturally infected goats from the states of Ceará, Rio Grande do Norte and Minas Gerais, Brazil, based on total nucleotide sequences of gag and pol genes. SRLV isolates from the microbiological collection of Sheep and Goat of Embrapa were cultured in goat synovial membrane. Supernatants containing cells were stored and viral particles were purified with sucrose density gradient centrifugation for RNA extraction. Then, sequencing in HiSeq 2500 platform (Illumina) was performed to obtain the total genome of the SRLV strains. The software MEGA version 7.0 was used for sequence editing and phylogeny analysis. A total of 17 isolates were multiplied in cellular culture and six were viable, which were two from Minas Gerais (MG), one from Rio Grande do Norte (RN) and three from Ceará (CE). Two sequences were obtained from the sequencing by synthesis, one from MG (BRMG CNPC) and one from RN (BRRN CNPC). From the total SRLV genome (9,200 bases), sample BRMG CNPC generated a consensus of 6,378 bases, approximately 69,34% of the viral genome and sample BRRN CNPC generated a consensus of 5,351 bases, which comprised 58.16% of the total genome. Samples in this study demonstrated closer phylogenetic relation with genotype B (B1 group). The remaining strains sequenced in Brazil are also present in the same group with the exception of three samples from genotype A. Immunodominant regions of gag gene and motifs of pol gene were similar to the standard CAEV Cork strain. For the first time, the complete gag and pol genes were sequenced in Brazil from the strain BRMG CNPC isolated in Minas Gerais state. In addition, 2490 bases from the total of 3316 bases were sequenced from the pol gene of the BRRN CNPC sample from Rio Grande do Norte. MenosAbstract: Small ruminant lentiviruses (SRLV) are widely distributed in the world and cause productive and economic losses. These pathogens have high genetic variability, which hinders diagnosis by molecular methods. Molecular characterization studies are good strategies for elaborating programs aimed to control or eradicate diseases. Hence, this study aimed to sequence, characterize and compare strains of SRLV, which were isolated from naturally infected goats from the states of Ceará, Rio Grande do Norte and Minas Gerais, Brazil, based on total nucleotide sequences of gag and pol genes. SRLV isolates from the microbiological collection of Sheep and Goat of Embrapa were cultured in goat synovial membrane. Supernatants containing cells were stored and viral particles were purified with sucrose density gradient centrifugation for RNA extraction. Then, sequencing in HiSeq 2500 platform (Illumina) was performed to obtain the total genome of the SRLV strains. The software MEGA version 7.0 was used for sequence editing and phylogeny analysis. A total of 17 isolates were multiplied in cellular culture and six were viable, which were two from Minas Gerais (MG), one from Rio Grande do Norte (RN) and three from Ceará (CE). Two sequences were obtained from the sequencing by synthesis, one from MG (BRMG CNPC) and one from RN (BRRN CNPC). From the total SRLV genome (9,200 bases), sample BRMG CNPC generated a consensus of 6,378 bases, approximately 69,34% of the viral genome and sample BR... Mostrar Tudo |
Palavras-Chave: |
Caracterização molecular; Molecular characterization; SRLV. |
Thesaurus NAL: |
Genome; Goats; Lentivirus; Phylogeny; Sheep. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 03206naa a2200337 a 4500 001 2112529 005 2019-11-07 008 2019 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.smallrumres.2019.06.011$2DOI 100 1 $aAZEVEDO, D. A. A. de 245 $aMolecular characterization of circulating strains of small ruminant lentiviruses in Brazil based on complete gag and pol genes.$h[electronic resource] 260 $c2019 520 $aAbstract: Small ruminant lentiviruses (SRLV) are widely distributed in the world and cause productive and economic losses. These pathogens have high genetic variability, which hinders diagnosis by molecular methods. Molecular characterization studies are good strategies for elaborating programs aimed to control or eradicate diseases. Hence, this study aimed to sequence, characterize and compare strains of SRLV, which were isolated from naturally infected goats from the states of Ceará, Rio Grande do Norte and Minas Gerais, Brazil, based on total nucleotide sequences of gag and pol genes. SRLV isolates from the microbiological collection of Sheep and Goat of Embrapa were cultured in goat synovial membrane. Supernatants containing cells were stored and viral particles were purified with sucrose density gradient centrifugation for RNA extraction. Then, sequencing in HiSeq 2500 platform (Illumina) was performed to obtain the total genome of the SRLV strains. The software MEGA version 7.0 was used for sequence editing and phylogeny analysis. A total of 17 isolates were multiplied in cellular culture and six were viable, which were two from Minas Gerais (MG), one from Rio Grande do Norte (RN) and three from Ceará (CE). Two sequences were obtained from the sequencing by synthesis, one from MG (BRMG CNPC) and one from RN (BRRN CNPC). From the total SRLV genome (9,200 bases), sample BRMG CNPC generated a consensus of 6,378 bases, approximately 69,34% of the viral genome and sample BRRN CNPC generated a consensus of 5,351 bases, which comprised 58.16% of the total genome. Samples in this study demonstrated closer phylogenetic relation with genotype B (B1 group). The remaining strains sequenced in Brazil are also present in the same group with the exception of three samples from genotype A. Immunodominant regions of gag gene and motifs of pol gene were similar to the standard CAEV Cork strain. For the first time, the complete gag and pol genes were sequenced in Brazil from the strain BRMG CNPC isolated in Minas Gerais state. In addition, 2490 bases from the total of 3316 bases were sequenced from the pol gene of the BRRN CNPC sample from Rio Grande do Norte. 650 $aGenome 650 $aGoats 650 $aLentivirus 650 $aPhylogeny 650 $aSheep 653 $aCaracterização molecular 653 $aMolecular characterization 653 $aSRLV 700 1 $aMONTEIRO, J. P. 700 1 $aPINHEIRO, R. R. 700 1 $aMUDADU, M. de A. 700 1 $aANDRIOLI, A. 700 1 $aARAÚJO, J. F. 700 1 $aSOUSA, A. L. M. de 700 1 $aSIDER, L. H. 700 1 $aPEIXOTO, R. M. 700 1 $aTEIXEIRA, M. F. da S. 773 $tSmall Ruminant Research$gv. 177, p. 160-166, 2019.
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