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Registro Completo |
Biblioteca(s): |
Embrapa Agricultura Digital. |
Data corrente: |
16/07/2009 |
Data da última atualização: |
23/09/2011 |
Autoria: |
IDE, C. D.; PEREIRA, M. G.; VIANA, A. P.; PEREIRA, T. N. S. |
Afiliação: |
CARLOS DAVID IDE, PESAGRO-RIO; MESSIAS GONZAGA PEREIRA, UENF; ALEXANDRE PIO VIANA, UENF; TELMA NAIR SANTANA PEREIRA, UENF. |
Título: |
Assessment of genetic diversity in a highly valuable medicinal plant Catharanthus roseus using molecular markers. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
Crop Breeding and Applied Biotechnology, Londrina, v. 9, n. 1, p. 52-59, Mar. 2009. |
Idioma: |
Inglês |
Conteúdo: |
Genetic diversity was evaluated among 14 cultivars of Catharanthus roseus using RAPD and ISSR markers.The RAPD primers resulted in the amplification of 56 bands, among which 46 (82%) bands were polymorphic Four ISSRprimers amplified 31 loci out of which 17 were polymorphic and 14 are monomorphic. The Jaccard's similarity derived from the combined marker system showed that the varieties First Kiss Coral and Cooler Orchid were the most closely relatedcultivars, with 98% similarity. In the dendrogram constructed on the basis of both RAPD and ISSR data two clear clusterswere obtained. The smaller cluster included C. roseus Cv Blue Pearl and C. roseus Cv. Patricia White and the larger clusterwas subdivided into two sub clusters with C. roseus Cv. First Kiss Polka Dot isolated from the rest of the cultivars. This maybe useful for breeding for improved quality. |
Palavras-Chave: |
Diversidade genética; ISSR; Marcadores moleculares; RAPD. |
Thesagro: |
Agricultura; Biotecnologia; Catharanthus Roseus; Genética; Variação Genética. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 01653naa a2200265 a 4500 001 1083160 005 2011-09-23 008 2009 bl uuuu u00u1 u #d 100 1 $aIDE, C. D. 245 $aAssessment of genetic diversity in a highly valuable medicinal plant Catharanthus roseus using molecular markers. 260 $c2009 520 $aGenetic diversity was evaluated among 14 cultivars of Catharanthus roseus using RAPD and ISSR markers.The RAPD primers resulted in the amplification of 56 bands, among which 46 (82%) bands were polymorphic Four ISSRprimers amplified 31 loci out of which 17 were polymorphic and 14 are monomorphic. The Jaccard's similarity derived from the combined marker system showed that the varieties First Kiss Coral and Cooler Orchid were the most closely relatedcultivars, with 98% similarity. In the dendrogram constructed on the basis of both RAPD and ISSR data two clear clusterswere obtained. The smaller cluster included C. roseus Cv Blue Pearl and C. roseus Cv. Patricia White and the larger clusterwas subdivided into two sub clusters with C. roseus Cv. First Kiss Polka Dot isolated from the rest of the cultivars. This maybe useful for breeding for improved quality. 650 $aAgricultura 650 $aBiotecnologia 650 $aCatharanthus Roseus 650 $aGenética 650 $aVariação Genética 653 $aDiversidade genética 653 $aISSR 653 $aMarcadores moleculares 653 $aRAPD 700 1 $aPEREIRA, M. G. 700 1 $aVIANA, A. P. 700 1 $aPEREIRA, T. N. S. 773 $tCrop Breeding and Applied Biotechnology, Londrina$gv. 9, n. 1, p. 52-59, Mar. 2009.
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Embrapa Agricultura Digital (CNPTIA) |
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