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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
18/04/2023 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VASQUEZ, D. D. N.; PINHEIRO, D. H.; TEIXEIRA, L. A.; MOREIRA-PINTO, C. E.; MACEDO, L. L. P. de; SALLES-FILHO, A. L. O.; SILVA, M. C. M. da; LOURENCO, I. T.; MORGANTE, C. V.; SILVA, L. P. da; SA, M. F. G. de. |
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Afiliação: |
DANIEL D. N. VASQUEZ, Catholic University of Brasília; DANIELE H. PINHEIRO; LAYS A. TEIXEIRA, Catholic University of Brasília; CLIDIA E. MOREIRA-PINTO; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; ALVARO L. O. SALLES-FILHO, Federal University of Paraná; MARIA CRISTINA MATTAR DA SILVA, Cenargen; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; LUCIANO PAULINO DA SILVA, Cenargen; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Simultaneous silencing of juvenile hormone metabolism genes through RNAi interrupts metamorphosis in the cotton boll weevil. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Frontiers in Molecular Biosciences, v. 10, 2023. |
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DOI: |
https://doi.org/10.3389/fmolb.2023.1073721 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Leonardo L. P. Macedo; Maria C. M. Silva; Isabela T. Lourenço-Tessutti; Luciano P. Silva; Maria F. Grossi-de-Sa. |
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Conteúdo: |
The cotton boll weevil (CBW) (Anthonomus grandis) is one of the major insect pests of cotton in Brazil. Currently, CBW control is mainly achieved by insecticide application, which is costly and insufficient to ensure effective crop protection. RNA interference (RNAi) has been used in gene function analysis and the development of insect control methods. However, some insect species respond poorly to RNAi, limiting the widespread application of this approach. Therefore, nanoparticles have been explored as an option to increase RNAi efficiency in recalcitrant insects. Herein, we investigated the potential of chitosan?tripolyphosphate (CS-TPP) and polyethylenimine (PEI) nanoparticles as a dsRNA carrier system to improve RNAi efficiency in the CBW. Different formulations of the nanoparticles with dsRNAs targeting genes associated with juvenile hormone metabolism, such as juvenile hormone diol kinase (JHDK), juvenile hormone epoxide hydrolase (JHEH), and methyl farnesoate hydrolase (MFE), were tested. The formulations were delivered to CBW larvae through injection (0.05?2 ?g), and the expression of the target genes was evaluated using RT-qPCR. PEI nanoparticles increased targeted gene silencing compared with naked dsRNAs (up to 80%), whereas CS-TPP-dsRNA nanoparticles decreased gene silencing (0%?20%) or led to the same level of gene silencing as the naked dsRNAs (up to 50%). We next evaluated the effects of targeting a single gene orsimultaneously targeting two genes via the injection of naked dsRNAs or dsRNAs complexed with PEI (500 ng) on CBW survival and phenotypes. Overall, the gene expression analysis showed that the treatments with PEI targeting either a single gene or multiple genes induced greater gene silencing than naked dsRNA (~60%). In addition, the injection of dsJHEH/JHDK, either naked or complexed with PEI, significantly affected CBW survival (18% for PEI nanoparticles and 47% for naked dsRNA) and metamorphosis. Phenotypic alterations, such as uncompleted pupation or malformed pupae, suggested that JHEH and JHDK are involved in developmental regulation. Moreover, CBW larvae treated with dsJHEH/JHDK + PEI (1,000 ng/g) exhibited significantly lower survival rate (55%) than those that MenosThe cotton boll weevil (CBW) (Anthonomus grandis) is one of the major insect pests of cotton in Brazil. Currently, CBW control is mainly achieved by insecticide application, which is costly and insufficient to ensure effective crop protection. RNA interference (RNAi) has been used in gene function analysis and the development of insect control methods. However, some insect species respond poorly to RNAi, limiting the widespread application of this approach. Therefore, nanoparticles have been explored as an option to increase RNAi efficiency in recalcitrant insects. Herein, we investigated the potential of chitosan?tripolyphosphate (CS-TPP) and polyethylenimine (PEI) nanoparticles as a dsRNA carrier system to improve RNAi efficiency in the CBW. Different formulations of the nanoparticles with dsRNAs targeting genes associated with juvenile hormone metabolism, such as juvenile hormone diol kinase (JHDK), juvenile hormone epoxide hydrolase (JHEH), and methyl farnesoate hydrolase (MFE), were tested. The formulations were delivered to CBW larvae through injection (0.05?2 ?g), and the expression of the target genes was evaluated using RT-qPCR. PEI nanoparticles increased targeted gene silencing compared with naked dsRNAs (up to 80%), whereas CS-TPP-dsRNA nanoparticles decreased gene silencing (0%?20%) or led to the same level of gene silencing as the naked dsRNAs (up to 50%). We next evaluated the effects of targeting a single gene orsimultaneously targeting two genes via the inj... Mostrar Tudo |
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Palavras-Chave: |
Bicudo do algodoeiro; Juvenile diol kinase; Juvenile hormone epoxide hydrolase; Polyethylenimine; Pragas do algodoeiro. |
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Thesagro: |
Algodão; Anthonomus Grandis; Inseto; Praga. |
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Thesaurus Nal: |
Chitosan; Cotton; Insect control; Insect pests. |
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Categoria do assunto: |
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O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1153217/1/fmolb-10-1073721.pdf
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Marc: |
LEADER 03612naa a2200421 a 4500
001 2153271
005 2023-05-25
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3389/fmolb.2023.1073721$2DOI
100 1 $aVASQUEZ, D. D. N.
245 $aSimultaneous silencing of juvenile hormone metabolism genes through RNAi interrupts metamorphosis in the cotton boll weevil.$h[electronic resource]
260 $c2023
500 $aNa publicação: Leonardo L. P. Macedo; Maria C. M. Silva; Isabela T. Lourenço-Tessutti; Luciano P. Silva; Maria F. Grossi-de-Sa.
520 $aThe cotton boll weevil (CBW) (Anthonomus grandis) is one of the major insect pests of cotton in Brazil. Currently, CBW control is mainly achieved by insecticide application, which is costly and insufficient to ensure effective crop protection. RNA interference (RNAi) has been used in gene function analysis and the development of insect control methods. However, some insect species respond poorly to RNAi, limiting the widespread application of this approach. Therefore, nanoparticles have been explored as an option to increase RNAi efficiency in recalcitrant insects. Herein, we investigated the potential of chitosan?tripolyphosphate (CS-TPP) and polyethylenimine (PEI) nanoparticles as a dsRNA carrier system to improve RNAi efficiency in the CBW. Different formulations of the nanoparticles with dsRNAs targeting genes associated with juvenile hormone metabolism, such as juvenile hormone diol kinase (JHDK), juvenile hormone epoxide hydrolase (JHEH), and methyl farnesoate hydrolase (MFE), were tested. The formulations were delivered to CBW larvae through injection (0.05?2 ?g), and the expression of the target genes was evaluated using RT-qPCR. PEI nanoparticles increased targeted gene silencing compared with naked dsRNAs (up to 80%), whereas CS-TPP-dsRNA nanoparticles decreased gene silencing (0%?20%) or led to the same level of gene silencing as the naked dsRNAs (up to 50%). We next evaluated the effects of targeting a single gene orsimultaneously targeting two genes via the injection of naked dsRNAs or dsRNAs complexed with PEI (500 ng) on CBW survival and phenotypes. Overall, the gene expression analysis showed that the treatments with PEI targeting either a single gene or multiple genes induced greater gene silencing than naked dsRNA (~60%). In addition, the injection of dsJHEH/JHDK, either naked or complexed with PEI, significantly affected CBW survival (18% for PEI nanoparticles and 47% for naked dsRNA) and metamorphosis. Phenotypic alterations, such as uncompleted pupation or malformed pupae, suggested that JHEH and JHDK are involved in developmental regulation. Moreover, CBW larvae treated with dsJHEH/JHDK + PEI (1,000 ng/g) exhibited significantly lower survival rate (55%) than those that
650 $aChitosan
650 $aCotton
650 $aInsect control
650 $aInsect pests
650 $aAlgodão
650 $aAnthonomus Grandis
650 $aInseto
650 $aPraga
653 $aBicudo do algodoeiro
653 $aJuvenile diol kinase
653 $aJuvenile hormone epoxide hydrolase
653 $aPolyethylenimine
653 $aPragas do algodoeiro
700 1 $aPINHEIRO, D. H.
700 1 $aTEIXEIRA, L. A.
700 1 $aMOREIRA-PINTO, C. E.
700 1 $aMACEDO, L. L. P. de
700 1 $aSALLES-FILHO, A. L. O.
700 1 $aSILVA, M. C. M. da
700 1 $aLOURENCO, I. T.
700 1 $aMORGANTE, C. V.
700 1 $aSILVA, L. P. da
700 1 $aSA, M. F. G. de
773 $tFrontiers in Molecular Biosciences$gv. 10, 2023.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Unidades Centrais. |
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Data corrente: |
24/08/2018 |
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Data da última atualização: |
22/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
FONSECA, C. E. L. da; MORAIS, F. M. de; GONCALVES, H. M.; AQUINO, F. de G.; ROCHA, F. S. |
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Afiliação: |
CARLOS EDUARDO LAZARINI DA FONSECA, CPAC; FERNANDA MONTEIRO DE MORAIS, CPAC; HELENICE MOURA GONCALVES, CPAC; FABIANA DE GOIS AQUINO, CPAC; FERNANDO SOUZA ROCHA, CPAC. |
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Título: |
Repeatability of fruit traits from two Hancornia speciosa populations from the core region of the Brazilian Cerrado. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 53, n. 6, p. 710-716, June 2018. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Repetibilidade de características de frutos de duas populações de Hancornia speciosa da região do Cerrado do Brasil. |
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Conteúdo: |
The objective of this work was to estimate the repeatability coefficients of mangaba (Hancornia speciosa) fruit traits, in order to define the number of fruit needed for an accurate selection of superior genotypes, as well as to conjecture about the nature of the phenotypic variation of these traits. Evaluations were performed for 160 fruit of 16 genotypes from two native H. speciosa populations of Goiás Velho and Padre Bernardo, in the state of Goiás, Brazil. Repeatability was estimated by the analysis of variance, principal component analyses based on covariance and on the correlation matrix, and structural analysis based on the correlation matrix. Repeatability estimates for fruit weight, diameter, and length, as well as seed number and weight, were of low magnitude, from 0.02 to 0.62, indicating low heritability. Repeatability estimates for ºBrix, titratable acidity, and ºBrix/acidity ratio were higher, from 0.34 to 0.91, indicating a low to potentially moderate heritability. The number of fruit for an effective selection of the best genotypes for titratable acidity, ºBrix, ºBrix/acidity ratio, and fruit weight is four for a 0.85 accuracy level. However, seven fruit would allow 0.90 accuracy for the same traits, and 0.85 accuracy for fruit length and diameter. The number and weight of seed per fruit are not effective for predicting the real value of a genotype. |
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Palavras-Chave: |
Coefficient of determination; Coeficiente de determinação; Eficiência seletiva; Medida repetida; Repeated measures. |
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Thesagro: |
Cerrado; Mangaba. |
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Thesaurus NAL: |
Savannas. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/181927/1/Repeatability-of-fruit-traits-from-two-Hancornia.pdf
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Marc: |
LEADER 02418naa a2200277 a 4500
001 2095788
005 2024-04-22
008 2018 bl uuuu u00u1 u #d
100 1 $aFONSECA, C. E. L. da
245 $aRepeatability of fruit traits from two Hancornia speciosa populations from the core region of the Brazilian Cerrado.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Repetibilidade de características de frutos de duas populações de Hancornia speciosa da região do Cerrado do Brasil.
520 $aThe objective of this work was to estimate the repeatability coefficients of mangaba (Hancornia speciosa) fruit traits, in order to define the number of fruit needed for an accurate selection of superior genotypes, as well as to conjecture about the nature of the phenotypic variation of these traits. Evaluations were performed for 160 fruit of 16 genotypes from two native H. speciosa populations of Goiás Velho and Padre Bernardo, in the state of Goiás, Brazil. Repeatability was estimated by the analysis of variance, principal component analyses based on covariance and on the correlation matrix, and structural analysis based on the correlation matrix. Repeatability estimates for fruit weight, diameter, and length, as well as seed number and weight, were of low magnitude, from 0.02 to 0.62, indicating low heritability. Repeatability estimates for ºBrix, titratable acidity, and ºBrix/acidity ratio were higher, from 0.34 to 0.91, indicating a low to potentially moderate heritability. The number of fruit for an effective selection of the best genotypes for titratable acidity, ºBrix, ºBrix/acidity ratio, and fruit weight is four for a 0.85 accuracy level. However, seven fruit would allow 0.90 accuracy for the same traits, and 0.85 accuracy for fruit length and diameter. The number and weight of seed per fruit are not effective for predicting the real value of a genotype.
650 $aSavannas
650 $aCerrado
650 $aMangaba
653 $aCoefficient of determination
653 $aCoeficiente de determinação
653 $aEficiência seletiva
653 $aMedida repetida
653 $aRepeated measures
700 1 $aMORAIS, F. M. de
700 1 $aGONCALVES, H. M.
700 1 $aAQUINO, F. de G.
700 1 $aROCHA, F. S.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 53, n. 6, p. 710-716, June 2018.
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Embrapa Cerrados (CPAC) |
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