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Registros recuperados : 61 | |
3. | | FERES, L. F.; PALHAO, M. P.; SIQUEIRA, L. G. B.; VIANA, J. H. M. Pregnancy losses after transfer of bovine embryos produced by assisted reproductive technologies. Reproduction, Fertility and Development, v. 31, n. 1, p. 167, 2019. Edição dos resumos da Annual Conference of the International Embryo Technology Society, New Orleans, Louisiana, 2019. Biblioteca(s): Embrapa Gado de Leite. |
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8. | | VIANA, J. H. M.; PALHÃO, M. P.; SIQUEIRA, L. G. B.; FONSECA, J. F. da; CAMARGO, L. S. de A. Ovarian follicular dynamics, follicle deviation, and oocyte yield in Gyr breed (Bos indicus) cows undergoing repeated ovum pick-up. Theriogenology, v. 73, n. 7, p. 966-972, 2010. Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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9. | | FONSECA, J. F. da; BRUSCHI, J. H.; ZAMBRINI, F. N.; DEMCZUK, E.; VIANA, J. H. M.; PALHÃO, M. P. Induction of synchronized estrus in dairy goats with different gonadotrophins. Animal Reproduction, Belo Horizonte, v. 2, n. 1, p. 50-53, jan./mar., 2005. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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11. | | PALHÃO, M. P.; PAGANINI FILHO, W.; VIANA, J. H. M.; GUIMARÃES, R. B.; FIGUEIREDO, A. C. S.; LUDGERO, B. F. A.; FERNANDES, C. A. de C. Acurácia da ultrassonografia e da avaliação comportamental na determinação das características funcionais de estruturas ovarianas císticas. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 49, n. 10, p.823-827, 2014. Notas Científicas Biblioteca(s): Embrapa Gado de Leite; Embrapa Unidades Centrais. |
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12. | | FERNANDES, C. A. DE C.; PALHÃO, M. P.; RIBEIRO, J. R.; VIANA, J. H. M.; GIOSO, M. M.; FIGUEIREDO, A. C. S.; OBA, E.; COSTA, D. S. Associação entre oxitetraciclina e cloprostenol no tratamento de vacas leiteiras com retenção de placenta. Revista Brasileira de Ciência Veterinária, v. 19, n. 3, p. 178-182, 2012. Biblioteca(s): Embrapa Gado de Leite. |
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13. | | CAMARGO, L. S. A.; ARASHIRO, E. K. N.; VIANA, J. H. M.; PALHÃO, M. P.; FONSECA, J. F. da; SÁ, W. F.; FERREIRA, A. M. Characteristics of calves produced by oocyte pick-up and in vitro fertilization. Arquivos de Ciências Veterinárias e Zoologia da UNIPAR, Umuarama, v. 7, p. 152, 2004. Suplemento 1. Edição da IX Jornada de Medicina Veterinária da UNIPAR e VI Mostra Científica em Medicina Veterinária, 2004. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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15. | | SIQUEIRA, L. G. B.; AREAS, V. S.; GHETTI, A. M.; FONSECA, J. F. da; PALHAO, M. P.; FERNANDES, C. A. C.; VIANA, J. H. M. Color Doppler flow imaging for the early detection of nonpregnant cattle at 20 days after timed artificial insemination. Journal of Dairy Science, v. 96, n. 10, p. 6461-6472, 2013. Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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16. | | GHETTI, A. M.; BRANDAO, F. Z.; SIQUEIRA, L. G. B.; AREAS, V. S.; ARASHIRO, E. K. N.; FERNANDES, C. A. C.; PALHAO, M. P.; VIANA, J. H. M. Comparação dos metodos e subjetivo de avaliação do padrão vascular do foliculo pre-ovulatorio utilizando-se Doppler colorido. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 26., 2012, Foz do Iguaçu. Anais. Belo Horizonte: CBRA, 2012. p. 478. Biblioteca(s): Embrapa Gado de Leite. |
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17. | | RIZZONI, L. B.; GARCIA, J. A. D.; PALHÃO, M. P.; MIYAUCHI, T. A.; RIBEIRO, S. D. DE A.; VIANA, J. H. M.; FERNANDES, C. A. DE C. Cloprostenol sódico no processo de involução uterina de cabras - resultados parciais. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, 25., 2011, Cumbuco. Resumos... p. 416 Biblioteca(s): Embrapa Gado de Leite. |
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18. | | GRÁZIA, J. G. V. de; ARASHIRO, E. K. N.; PALHAO, M. P.; CAMARGO, L. S. de A.; FERNANDES, C. A. C.; VIANA, J. H. M. Is it possible to increase the efficiency of in vitro embryo production in Holstein preselecting donors with more oocyte production? Animal Reproduction, v.10, n.3, p. 526, Jul./Sept. 2013. Biblioteca(s): Embrapa Gado de Leite. |
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19. | | VIANA, J. H. M.; ARASHIRO, E. K. N.; SIQUEIRA, L. G. B.; PALHAO, M. P.; CAMARGO, L. S. de A.; FERNANDES, C. A. C. Manejo de riscos associados à aspiração folicular orientada por ultrassonografia em bovinos. Spermova, v. 3, n. 1, p. 11-14, 2013. Biblioteca(s): Embrapa Gado de Leite. |
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20. | | WOHLRES-VIANA, S.; ARASHIRO, E. K. N; MACHADO, M. A.; CAMARGO, L. S. de A.; SIQUEIRA, L. G. B.; PALHÃO, M. P.; VIANA, J. H. M. Intrafollicular oestradiol production, expression of the LH receptor (LHR) gene and its isoforms, and early follicular deviation in Bos indicus. Reproduction Fertility and Development, v. 29, n. 10, p. 1958-1970, 2017. Publicado online em 19 nov. 2016. Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 61 | |
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Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
09/01/2019 |
Data da última atualização: |
12/02/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 2 |
Autoria: |
VASCONCELOS, M. J. V. de; ANTUNES, M. S.; OLIVEIRA, M. F. de; LOPES, M. A.; FIGUEIREDO, J. E. F. |
Afiliação: |
MARIA JOSE VILACA DE VASCONCELOS, CNPMS; MAURICIO SCHUSTERSCHITZ ANTUNES, Colorado State University; MAURILIO FERNANDES DE OLIVEIRA, CNPMS; MAURICIO ANTONIO LOPES, DE/PR; JOSE EDSON FONTES FIGUEIREDO, CNPMS. |
Título: |
Callus induction and plant regeneration from immature embryos culture of tropical maize. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 17, n. 3, p. 359-368, 2018. |
Idioma: |
Inglês |
Conteúdo: |
The development of protocols to overcome the current limitations of callus induction and in vitro regeneration of highly recalcitrant tropical maize is crucial for plant genetic transformation. The ability of embryogenic callus (EC) formation of 46 tropical maize hybrids and 14 inbred lines was tested using N6 medium with the following changes: medium A (N6 + 15 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium B (N6 + 30 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium C (N6+ 30 µM dicamba + 6 mM L-proline), and medium D (N6+ 30 µM dicamba + 25 mM L-proline). Compact (Type I) and friable (Type II) callus were induced in the four media. Twenty genotypes produced callus in all media, and four genotypes (CO32, AG8012, CMS477BC4F2, and CMS-HGZ10) produced the highest number of callus (114, 134, 131, and 126 calli, respectively). All immature embryos of ten genotypes produced callus in at least one medium, while eight genotypes were highly recalcitrant and they did not produce any callus. The frequencies of EC ranged from 0% to 38%, and the highest rate of EC was observed on medium B (0.40) with a total of 865 calli, and the lowest induction rate was obtained with medium D (0.29) with 555 calli (P= 0.05). From the seventy-two EC of 26 genotypes transferred to Murashige & Skoog regeneration medium, twenty-four (66.7%) differentiated into green plants which produced seeds in R0 and R1 generations, and twelve (33.3%) developed into albino plants. The results demonstrated that the problem of the recalcitrant genotypes can be, at least partially, overcome by using immature embryos as explants together with tissue culture media formulations. Keywords: Zea mays, immature embryos, somatic embryogenesis, tissue culture, type I and type II calli. MenosThe development of protocols to overcome the current limitations of callus induction and in vitro regeneration of highly recalcitrant tropical maize is crucial for plant genetic transformation. The ability of embryogenic callus (EC) formation of 46 tropical maize hybrids and 14 inbred lines was tested using N6 medium with the following changes: medium A (N6 + 15 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium B (N6 + 30 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium C (N6+ 30 µM dicamba + 6 mM L-proline), and medium D (N6+ 30 µM dicamba + 25 mM L-proline). Compact (Type I) and friable (Type II) callus were induced in the four media. Twenty genotypes produced callus in all media, and four genotypes (CO32, AG8012, CMS477BC4F2, and CMS-HGZ10) produced the highest number of callus (114, 134, 131, and 126 calli, respectively). All immature embryos of ten genotypes produced callus in at least one medium, while eight genotypes were highly recalcitrant and they did not produce any callus. The frequencies of EC ranged from 0% to 38%, and the highest rate of EC was observed on medium B (0.40) with a total of 865 calli, and the lowest induction rate was obtained with medium D (0.29) with 555 calli (P= 0.05). From the seventy-two EC of 26 genotypes transferred to Murashige & Skoog regeneration medium, twenty-four (66.7%) differentiated into green plants which produced seeds in R0 and R1 generations, and twelve (33.3%) developed into albino plants. The results demonstrated that ... Mostrar Tudo |
Palavras-Chave: |
Calos tipo I e tipo II; Embriogênese somática. |
Thesagro: |
Cultura de Tecido; Embrião Vegetal; Zea Mays. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/190064/1/Callus-induction.pdf
|
Marc: |
LEADER 02500naa a2200229 a 4500 001 2103395 005 2019-02-12 008 2018 bl uuuu u00u1 u #d 100 1 $aVASCONCELOS, M. J. V. de 245 $aCallus induction and plant regeneration from immature embryos culture of tropical maize.$h[electronic resource] 260 $c2018 520 $aThe development of protocols to overcome the current limitations of callus induction and in vitro regeneration of highly recalcitrant tropical maize is crucial for plant genetic transformation. The ability of embryogenic callus (EC) formation of 46 tropical maize hybrids and 14 inbred lines was tested using N6 medium with the following changes: medium A (N6 + 15 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium B (N6 + 30 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium C (N6+ 30 µM dicamba + 6 mM L-proline), and medium D (N6+ 30 µM dicamba + 25 mM L-proline). Compact (Type I) and friable (Type II) callus were induced in the four media. Twenty genotypes produced callus in all media, and four genotypes (CO32, AG8012, CMS477BC4F2, and CMS-HGZ10) produced the highest number of callus (114, 134, 131, and 126 calli, respectively). All immature embryos of ten genotypes produced callus in at least one medium, while eight genotypes were highly recalcitrant and they did not produce any callus. The frequencies of EC ranged from 0% to 38%, and the highest rate of EC was observed on medium B (0.40) with a total of 865 calli, and the lowest induction rate was obtained with medium D (0.29) with 555 calli (P= 0.05). From the seventy-two EC of 26 genotypes transferred to Murashige & Skoog regeneration medium, twenty-four (66.7%) differentiated into green plants which produced seeds in R0 and R1 generations, and twelve (33.3%) developed into albino plants. The results demonstrated that the problem of the recalcitrant genotypes can be, at least partially, overcome by using immature embryos as explants together with tissue culture media formulations. Keywords: Zea mays, immature embryos, somatic embryogenesis, tissue culture, type I and type II calli. 650 $aCultura de Tecido 650 $aEmbrião Vegetal 650 $aZea Mays 653 $aCalos tipo I e tipo II 653 $aEmbriogênese somática 700 1 $aANTUNES, M. S. 700 1 $aOLIVEIRA, M. F. de 700 1 $aLOPES, M. A. 700 1 $aFIGUEIREDO, J. E. F. 773 $tRevista Brasileira de Milho e Sorgo, Sete Lagoas$gv. 17, n. 3, p. 359-368, 2018.
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