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Registro Completo |
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Meio Ambiente. |
Data corrente: |
16/10/1997 |
Data da última atualização: |
17/07/2017 |
Autoria: |
LIMA, M. A. de. |
Afiliação: |
MAGDA APARECIDA DE LIMA, CNPMA. |
Título: |
Planejamento urbano: utilização de Sistema de Informação Geográfica - SIG na avaliação socioeconômica e ecológica - um estudo de caso. |
Ano de publicação: |
1997 |
Fonte/Imprenta: |
In: ROMEIRO, A.R.; REYDON, B.P.; LEONARDI, M.L.A. Org.). Economia do meio ambiente: teoria, políticas e a gestão de espaços regionais. Campinas: UNICAMP-IE, 1997. |
Páginas: |
p.219-239 |
ISBN: |
85-86215-04-X |
Idioma: |
Português |
Conteúdo: |
Planejamento: medida de prevenção e controle da degradação ambiental. Recuperação ambiental: medida corretiva da degradação ambiental. |
Palavras-Chave: |
Custos; Desapropriações; Economia - meio ambiente; Legislação ambiental; Planejamento ambiental; Planejamento urbano; Preservação; Recuperação ambiental; Recuperação de solos; Recursos naturais; Resíduos sólidos; Vegetação natural. |
Thesagro: |
Degradação Ambiental. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/146719/1/1997PL012-Lima-Planejamento-3337.pdf
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Marc: |
LEADER 01196naa a2200301 a 4500 001 1012737 005 2017-07-17 008 1997 bl uuuu u00u1 u #d 020 $a85-86215-04-X 100 1 $aLIMA, M. A. de 245 $aPlanejamento urbano$butilização de Sistema de Informação Geográfica - SIG na avaliação socioeconômica e ecológica - um estudo de caso. 260 $c1997 300 $ap.219-239 520 $aPlanejamento: medida de prevenção e controle da degradação ambiental. Recuperação ambiental: medida corretiva da degradação ambiental. 650 $aDegradação Ambiental 653 $aCustos 653 $aDesapropriações 653 $aEconomia - meio ambiente 653 $aLegislação ambiental 653 $aPlanejamento ambiental 653 $aPlanejamento urbano 653 $aPreservação 653 $aRecuperação ambiental 653 $aRecuperação de solos 653 $aRecursos naturais 653 $aResíduos sólidos 653 $aVegetação natural 773 $tIn: ROMEIRO, A.R.; REYDON, B.P.; LEONARDI, M.L.A. Org.). Economia do meio ambiente: teoria, políticas e a gestão de espaços regionais. Campinas: UNICAMP-IE, 1997.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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Registro Completo
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
12/09/2022 |
Data da última atualização: |
12/04/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
ANGELO, P. C. da S.; PEREIRA, L. F. P.; SERA, G. H.; SETA, T. |
Afiliação: |
PAULA CRISTINA DA SILVA ANGELO, CNPCa. |
Título: |
Coffee microspore cultivation to attain doubled-haploid stable plantlets. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
INTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França. |
DOI: |
10.17660/ActaHortic.2023.1359.14 |
Idioma: |
Inglês |
Notas: |
E-poster. |
Conteúdo: |
The aim of this study was to explore the potentials of progenies produced by the introgression of disease resistance from related Coffea species to C. arabica . In the IDR-Parana, Brazil, hybrids of C. arabica (tetraploid) x C. racemosa (diploid) were obtained. Self-pollination of some of these hybrids produced very mild yields, despite the backcros to C. arabica . In an attempt to regenerate stable double-haploids, anthers and isolated microspores were grown in vitro . It was assumed that chromosomes captured in a microspore after meiosis can acquire homo and/or homeologous stability faster in vitro than in vivo. C. arabica anthers served as control. Flowers were collected when the young microspores were uninucleated, and disinfected with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer that worked for a few seconds, washed and centrifuged twice at 100xg and grown in modified N6 liquid medium (10 5 cells/mL in 35 mm diameter plates). Explants were grown continuously for six months on the same N6 medium containing 6.5 mg/L auxins, 1.0 mg/L cytokinins and 0.5 mg/L gibberellin (GA3), at 27 o C in the dark. Isolated microspores produced embryo-like structures or microcalli at very low frequencies (0.3 per plate). The anthers, on the other hand, produced embryogenic calli . One particular endogenous fungus infected 70% of the control anthers but 5% produced friable calli . Some of these were embryogenic, but arrested when compared to calli produced by the interspecific hybrid anthers. Regarding the hybrid anthers, 25% were infected by a same endogenous bacteria but 8% produced embryogenic tissue, with globular embryos multiplying and maturing simultaneously upon transfer to N6 medium without growth regulators. After 1.2 years, the photomorphogenesis has been induced under light, and auxin/cytokinin ratio = 2 plus GA3. MenosThe aim of this study was to explore the potentials of progenies produced by the introgression of disease resistance from related Coffea species to C. arabica . In the IDR-Parana, Brazil, hybrids of C. arabica (tetraploid) x C. racemosa (diploid) were obtained. Self-pollination of some of these hybrids produced very mild yields, despite the backcros to C. arabica . In an attempt to regenerate stable double-haploids, anthers and isolated microspores were grown in vitro . It was assumed that chromosomes captured in a microspore after meiosis can acquire homo and/or homeologous stability faster in vitro than in vivo. C. arabica anthers served as control. Flowers were collected when the young microspores were uninucleated, and disinfected with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer that worked for a few seconds, washed and centrifuged twice at 100xg and grown in modified N6 liquid medium (10 5 cells/mL in 35 mm diameter plates). Explants were grown continuously for six months on the same N6 medium containing 6.5 mg/L auxins, 1.0 mg/L cytokinins and 0.5 mg/L gibberellin (GA3), at 27 o C in the dark. Isolated microspores produced embryo-like structures or microcalli at very low frequencies (0.3 per plate). The anthers, on the other hand, produced embryogenic calli . One particular endogenous fungus infected 70% of the control anthers but 5% produced friable calli . Some of these were embryogenic, but arrested when compared to calli prod... Mostrar Tudo |
Thesagro: |
Café; Embriogénese; Pólen; Rubiaceae. |
Thesaurus NAL: |
Embryogenesis; Pollen. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/249059/1/Coffee-microspore-cultivation.pdf
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Marc: |
LEADER 02577nam a2200241 a 4500 001 2146372 005 2023-04-12 008 2022 bl uuuu u00u1 u #d 024 7 $a10.17660/ActaHortic.2023.1359.14$2DOI 100 1 $aANGELO, P. C. da S. 245 $aCoffee microspore cultivation to attain doubled-haploid stable plantlets.$h[electronic resource] 260 $aINTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França.$c2022 500 $aE-poster. 520 $aThe aim of this study was to explore the potentials of progenies produced by the introgression of disease resistance from related Coffea species to C. arabica . In the IDR-Parana, Brazil, hybrids of C. arabica (tetraploid) x C. racemosa (diploid) were obtained. Self-pollination of some of these hybrids produced very mild yields, despite the backcros to C. arabica . In an attempt to regenerate stable double-haploids, anthers and isolated microspores were grown in vitro . It was assumed that chromosomes captured in a microspore after meiosis can acquire homo and/or homeologous stability faster in vitro than in vivo. C. arabica anthers served as control. Flowers were collected when the young microspores were uninucleated, and disinfected with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer that worked for a few seconds, washed and centrifuged twice at 100xg and grown in modified N6 liquid medium (10 5 cells/mL in 35 mm diameter plates). Explants were grown continuously for six months on the same N6 medium containing 6.5 mg/L auxins, 1.0 mg/L cytokinins and 0.5 mg/L gibberellin (GA3), at 27 o C in the dark. Isolated microspores produced embryo-like structures or microcalli at very low frequencies (0.3 per plate). The anthers, on the other hand, produced embryogenic calli . One particular endogenous fungus infected 70% of the control anthers but 5% produced friable calli . Some of these were embryogenic, but arrested when compared to calli produced by the interspecific hybrid anthers. Regarding the hybrid anthers, 25% were infected by a same endogenous bacteria but 8% produced embryogenic tissue, with globular embryos multiplying and maturing simultaneously upon transfer to N6 medium without growth regulators. After 1.2 years, the photomorphogenesis has been induced under light, and auxin/cytokinin ratio = 2 plus GA3. 650 $aEmbryogenesis 650 $aPollen 650 $aCafé 650 $aEmbriogénese 650 $aPólen 650 $aRubiaceae 700 1 $aPEREIRA, L. F. P. 700 1 $aSERA, G. H. 700 1 $aSETA, T.
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