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Registro Completo |
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
25/09/2008 |
Data da última atualização: |
25/09/2008 |
Autoria: |
DECAENS, T.; ROUGERIE, R.; RICHARD, B.; JAMES, S.; HEBERT, P. |
Título: |
A taxonomic survey of Upper-Normandy earthorms with DNA barecodes. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM. |
Idioma: |
Inglês |
Conteúdo: |
Earthworms are represented in most terrestrial ecosystems, where they perform key ecological
functions. Their relatively large body-size facilitates their sampling and their study when compared
with smaller groups of the soil fauna. Despite these characteristics, taxonomic knowledge of
these organisms is still weak, even for the family Lumbricidae which is the exclusive representative
of the group in Western Europe. Identification difficulties are particularly dramatic for juveniles
for which the classical diagnostic characters (structure and position of the clitellum) are absent.
The main objective of this study was to survey the taxonomy of earthworm species in Upper
Normandy by building a database of DNA barcodes (partial sequences of the mitochondrial
gene COI). In April 2007 and 2008, we made an exhaustive collection of individuals (about 20
per species) of the species pool of the Upper Normandy region (ca. 20 species). Special attention
was paid to inclusion of intraspecific phenotypic variability, by sampling specimens varying in
size and coloration, two characters usually neglected in earthworm taxonomy though they have
been shown to be potential indicators of cryptic diversity. Small fragments of cutaneous tissues
were sampled from each specimen before its fixation in formaldehyde and storage in alcohol.
Sequencing of the COI gene was performed at the Canadian Centre for DNA Barcoding, and
results were analysed through the BOLD bioinformatics platform (Ratnasingham & Hebert 2007).
The results allow exploration of some more specific aspects of earthworm taxonomy, such as
the diversity of local species that are known as invasive in other regions; examining some
taxonomic problems raised by polymorphic species suspected to be complex of cryptic species;
verifying the status of potential cryptic species revealed by DNA barcoding. Preliminary results
indicate that three of the most common species in the target area (Lumbricus terrestris,
Allolobophora chlorotica and Aporrectodea caliginosa) all represent complexes of cryptic
species. MenosEarthworms are represented in most terrestrial ecosystems, where they perform key ecological
functions. Their relatively large body-size facilitates their sampling and their study when compared
with smaller groups of the soil fauna. Despite these characteristics, taxonomic knowledge of
these organisms is still weak, even for the family Lumbricidae which is the exclusive representative
of the group in Western Europe. Identification difficulties are particularly dramatic for juveniles
for which the classical diagnostic characters (structure and position of the clitellum) are absent.
The main objective of this study was to survey the taxonomy of earthworm species in Upper
Normandy by building a database of DNA barcodes (partial sequences of the mitochondrial
gene COI). In April 2007 and 2008, we made an exhaustive collection of individuals (about 20
per species) of the species pool of the Upper Normandy region (ca. 20 species). Special attention
was paid to inclusion of intraspecific phenotypic variability, by sampling specimens varying in
size and coloration, two characters usually neglected in earthworm taxonomy though they have
been shown to be potential indicators of cryptic diversity. Small fragments of cutaneous tissues
were sampled from each specimen before its fixation in formaldehyde and storage in alcohol.
Sequencing of the COI gene was performed at the Canadian Centre for DNA Barcoding, and
results were analysed through the BOLD bioinformatics platform (Ratnasingham ... Mostrar Tudo |
Categoria do assunto: |
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Marc: |
LEADER 02787naa a2200169 a 4500 001 1314944 005 2008-09-25 008 2008 bl uuuu u00u1 u #d 100 1 $aDECAENS, T. 245 $aA taxonomic survey of Upper-Normandy earthorms with DNA barecodes. 260 $c2008 520 $aEarthworms are represented in most terrestrial ecosystems, where they perform key ecological functions. Their relatively large body-size facilitates their sampling and their study when compared with smaller groups of the soil fauna. Despite these characteristics, taxonomic knowledge of these organisms is still weak, even for the family Lumbricidae which is the exclusive representative of the group in Western Europe. Identification difficulties are particularly dramatic for juveniles for which the classical diagnostic characters (structure and position of the clitellum) are absent. The main objective of this study was to survey the taxonomy of earthworm species in Upper Normandy by building a database of DNA barcodes (partial sequences of the mitochondrial gene COI). In April 2007 and 2008, we made an exhaustive collection of individuals (about 20 per species) of the species pool of the Upper Normandy region (ca. 20 species). Special attention was paid to inclusion of intraspecific phenotypic variability, by sampling specimens varying in size and coloration, two characters usually neglected in earthworm taxonomy though they have been shown to be potential indicators of cryptic diversity. Small fragments of cutaneous tissues were sampled from each specimen before its fixation in formaldehyde and storage in alcohol. Sequencing of the COI gene was performed at the Canadian Centre for DNA Barcoding, and results were analysed through the BOLD bioinformatics platform (Ratnasingham & Hebert 2007). The results allow exploration of some more specific aspects of earthworm taxonomy, such as the diversity of local species that are known as invasive in other regions; examining some taxonomic problems raised by polymorphic species suspected to be complex of cryptic species; verifying the status of potential cryptic species revealed by DNA barcoding. Preliminary results indicate that three of the most common species in the target area (Lumbricus terrestris, Allolobophora chlorotica and Aporrectodea caliginosa) all represent complexes of cryptic species. 700 1 $aROUGERIE, R. 700 1 $aRICHARD, B. 700 1 $aJAMES, S. 700 1 $aHEBERT, P. 773 $tIn: INTERNATIONAL COLLOQUIUM ON SOIL ZOOLOGY, 15; INTERNATIONAL COLLOQUIUM ON APTERYGOTA, 12., 2008, Curitiba. Biodiversity, conservation and sustainabele management of soil animal: abstracts. Colombo: Embrapa Florestas. Editors: George Gardner Brown; Klaus Dieter Sautter; Renato Marques; Amarildo Pasini. 1 CD-ROM.
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Embrapa Florestas (CNPF) |
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Registro Completo
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
16/11/2017 |
Data da última atualização: |
16/11/2017 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
REIS, A. M.; CARLI, J. D.; VELOZA, Z. M.; BONFIM, G. D.; BARTELEGA, L.; FERREIRA, I. B.; BORATO, P. B.; TAVARES, M.; CARVALHO, C. H. S. de; PAIVA, A. C. R. S.; ANGELO, P. C. da S. |
Afiliação: |
Bolsista Fundação Procafé; Bolsista SAPC na Fundação Procafé; Estagiária UNIS na Fundação Procafé; Estagiária Fundação Procafé; Bolsista SAPC na Fundação Procafé; Fundação Procafé; Bolsista SAPC na Fundação Procafé; Fundação Procafé; CARLOS HENRIQUE S DE CARVALHO, SAPC; Fundação Procafé; PAULA CRISTINA DA SILVA ANGELO, SAPC. |
Título: |
Métodos de manejo de viroplantas de cafeeiro arabica aos oito meses de aclimatização para a produção de micro-estacas. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE PESQUISAS CAFEEIRAS, 43., 2017. Poços de Caldas, 2017. Novas Tecnologias difundir, pro bom café produzir. Brasília, DF: Embrapa Café, 2017. |
Idioma: |
Português |
Conteúdo: |
Os custos de produção de vitroplantas de cafeeiro ainda são elevados e dificultam a sua utilização como mudas ou como passo inicial para a multiplicação de genótipos melhorados, selecionados em campo. Este trabalho teve como objetivo comparar três métodos de manejo de vitroplantas aclimatizadas que poderão contribuir para a redução dos custos da micropropagação, facilitando a inserção das vitroplantas na cadeia produtiva do cafeeiro. Vitroplantas de cafeeiro arábica Catucaí (567), produzidas por embriogênese somática induzida em tecidos de folha seguindo protocolo utilizado no Laboratório de Cultura de Tecidos da Fundação Procafé, Varginha - MG, foram transplantadas para substrato de fibra de coco e mantidas em casa de vegetação, com sistema de nebulização automatizado e temperatura controlada, por três meses para aclimatização. |
Palavras-Chave: |
Aclimatização; Embriogênese somática. |
Thesagro: |
Café; Coffea arabica; Estufa; Micropropagação; Reprodução vegetal. |
Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166762/1/Metodos-de-manejo-de-vitroplantas-de-cafeeiro-arabica.pdf
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Marc: |
LEADER 01926nam a2200313 a 4500 001 2079812 005 2017-11-16 008 2017 bl uuuu u00u1 u #d 100 1 $aREIS, A. M. 245 $aMétodos de manejo de viroplantas de cafeeiro arabica aos oito meses de aclimatização para a produção de micro-estacas.$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE PESQUISAS CAFEEIRAS, 43., 2017. Poços de Caldas, 2017. Novas Tecnologias difundir, pro bom café produzir. Brasília, DF: Embrapa Café$c2017 520 $aOs custos de produção de vitroplantas de cafeeiro ainda são elevados e dificultam a sua utilização como mudas ou como passo inicial para a multiplicação de genótipos melhorados, selecionados em campo. Este trabalho teve como objetivo comparar três métodos de manejo de vitroplantas aclimatizadas que poderão contribuir para a redução dos custos da micropropagação, facilitando a inserção das vitroplantas na cadeia produtiva do cafeeiro. Vitroplantas de cafeeiro arábica Catucaí (567), produzidas por embriogênese somática induzida em tecidos de folha seguindo protocolo utilizado no Laboratório de Cultura de Tecidos da Fundação Procafé, Varginha - MG, foram transplantadas para substrato de fibra de coco e mantidas em casa de vegetação, com sistema de nebulização automatizado e temperatura controlada, por três meses para aclimatização. 650 $aCafé 650 $aCoffea arabica 650 $aEstufa 650 $aMicropropagação 650 $aReprodução vegetal 653 $aAclimatização 653 $aEmbriogênese somática 700 1 $aCARLI, J. D. 700 1 $aVELOZA, Z. M. 700 1 $aBONFIM, G. D. 700 1 $aBARTELEGA, L. 700 1 $aFERREIRA, I. B. 700 1 $aBORATO, P. B. 700 1 $aTAVARES, M. 700 1 $aCARVALHO, C. H. S. de 700 1 $aPAIVA, A. C. R. S. 700 1 $aANGELO, P. C. da S.
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