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| Registros recuperados : 317 | |
| 141. |  | ANDREOTTI, R.; GOMES, A.; PIRES, P. P.; RIVERA, F. E. B. Planejamento sanitario de gado de corte. In: CURSO DE MANEJO SANITARIO, REPRODUTIVO E NUTRICIONAL EM BOVINOS, Programa Repasto - Seprotur, turma 5 - 2. etapa, 2003, Campo Grande. Palestras apresentadas. Campo Grande: Embrapa Gado de Corte, 2003. p. 72-80. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 142. | | OLIVEIRA, J. M. de; MATOS, M. de F. C.; OSHIRO, L. M.; ANDREOTTI, R. Prevalence of anti-Neospora caninum antibodies in dogs in the urban area of Campo Grande, MS, Brazil. Revista Brasileira de Parasitologia Veterinária, São Paulo, v.13, n. 4, p.155-158, out./dez. 2004. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 145. | | ANDREOTTI, R.; GARCIA, M. V.; CUNHA, R. C.; BARROS, J. C. Protective action of Tagetes minuta (Asteraceae) essential oil in the control of Rhipicephalus microplus (Canestrini, 1887) (Acari: Ixodidae) in a cattle pen trial. Veterinary Parasitology, v.197, n. 1-2, out., 2013, p. 341-345| Biblioteca(s): Embrapa Gado de Corte. |
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| 146. | | OSHIRO, L. M.; BARROS, J. C.; GRECCO, B.; GONÇALVES, K. N.; ANDREOTTI, R. Avaliação sorológica para Neospora caninum em fêmeas de um rebanho ovino de cria em Campo Grande - Mato Grosso do Sul. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 15.; SEMINÁRIO DE PARASITOLOGIA VETERINÁRIA DOS PAÍSES DO MERCOSUL, 2., 2008., Curitiba. Programa & Resumos. Jaboticabal: CBPV, 2008. 1 p. 1 CD-ROM. Resumos_Apresentação ORAL . Código O-051| Biblioteca(s): Embrapa Gado de Corte. |
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| 148. | | ANDREOTTI, R.; GARCIA, M. V.; HIGA, L. O. de S.; PIÑA, F. T. B. Biology, Ecology and Importance of Rhipicephalus Ticks in Brazil. In: KUMAR, S.; BAVUGAR, R. C.; SHARMA, A. K.; MIRANDA, E. M.; CHAUBEY, A. K. (ed.) The Entomological Guide to Rhipicephalus. Nova York: Nova Science Publishers, 2021. p. 1-14. (Insects and Other Terrestrial Arthropods: Biology, Chemistry and Behavior)| Biblioteca(s): Embrapa Gado de Corte. |
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| 150. | | SANTOS JUNIOR, A. G.; CUNHA, R. C.; ANDREOTTI, R.; LEITE, F. P. L. Avaliação de diferentes métodos de purificação da proteína bm86-cg expressa em pichia pastoris. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., 2010, Campo Grande, MS. [Anais...]. Campo Grande, MS: Colégio Brasileiro de Parasitologia Veterinária, 2010. 1 CD-ROM. 1 p.| Biblioteca(s): Embrapa Gado de Corte. |
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| 151. | | CUNHA, R.; LEITÃO, A.; LEITE, F.; BARROS, J. C.; ANDREOTTI, R. Associação de OprI à rBmCG na modulação imune para imunização contra carrapato bovino. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 17., 2012, São Luis. Parasitologia veterinária, bem estar e produção animal: anais. São Luis: Colégio Brasileiro de Parasitologia Veterinária, 2012. p.174 PA081| Biblioteca(s): Embrapa Gado de Corte. |
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| 153. | | SOARES, M. A.; ANDREOTTI, R.; BARROS, J. C.; MELLO, A. K. M. Eficiência in vitro de acaricidas em cepas de campo de carrapato-do-boi. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., 2010, Campo Grande, MS. [Anais...]. Campo Grande, MS: Colégio Brasileiro de Parasitologia Veterinária, 2010. 1 CD-ROM. 1 p.| Biblioteca(s): Embrapa Gado de Corte. |
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| 157. | | ANDREOTTI, R.; PINKNEY, R. D.; PIRES, P. P.; SILVA, E. A. E. Evidence of Neospora canimum in beef cattle and dogs in the State of Mato Grosso do Sul, Center-Western Region, Brazil. Revista Brasileira de Parasitologia Veterinária, São Paulo, v. 13, n. 3, p. 129-131, jul./set. 2004. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 158. | | HIGA, L. de O. S.; GARCIA, M. V.; BARROS, J. C.; KOLLER, W. W.; ANDREOTTI, R. Evaluation of Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) resistance to different acaricide formulations using samples from brazilian properties. Revista Brasileira de Parasitologia Veterinária , Jaboticabal, v. 25, n. 2, p. 163-171, abr./jul. 2016.| Biblioteca(s): Embrapa Gado de Corte. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
22/03/2011 |
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Data da última atualização: |
09/09/2014 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
ANDREOTTI, R.; SOARES, M.; CUNHA, R. C.; LEITE, F. P. L. |
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Afiliação: |
RENATO ANDREOTTI E SILVA, CNPGC; UFPEL; UFPEL; UFPEL. |
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Título: |
Heterologous expression in Pichia pastoris of gene BMTI optimized. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONGRESS OF PARASITOLOGY, 12., 2010, Melbourne. Conference abstracts. Melbourne: The Australian Society for Parasitology:World Federation of Parasitologists, 2010. |
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Páginas: |
p.149-152 |
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Idioma: |
Inglês
Português |
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Notas: |
ICOPA XII |
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Conteúdo: |
The cattle tick, Rhipicephalus microplus, is the main tick species that undermines productivity losses in livestock resulting in the production of milk and meat throughout Brazil. Its control is primarily chemical and faces problems due to the resistance of ticks to several active principles used. The immune control is a viable alternative to tick control, and the protein BMTI is a strong candidate as a vaccine antigen. The aim of this work was to synthesize the sequencing of BMTI (BmTIS), optimizing it to "codon usage" of Pichia pastoris in an attempt to improve its expression. This sequence was cloned in eukaryotic expression vector pPICZaA, respecting the make reading the AOX1 promoter, present in the plasmid and that is strongly induced by the presence of methanol, following the sign "a-factor for protein secretion, and the tail of poly-histidine. The resulting plasmid, pPICZaBmTIS, X33 was transformed into strains of P. pastoris. Clones were characterized by PCR using primers of the vector and also by dot blot analysis using anti-histidine. A clone (X33BmTIS17) was sequenced and selected to be induced. A single colony clone X33BmTIS17 was inoculated with 25mL of minimal medium with glycerol and incubated at 200rpm, 300C, overnight. The culture was centrifuged at 1500 g, 5min and resuspended in minimal medium with methanol. The supernatant was subjected to SDS-PAGE 0.7%, which showed a major band with the approximate size of 49 kDa. In Western bloting, a band at the same time was recognized by the anti-histidine and by bovine serum immunized with a synthetic based pepitídeo (BmTI). Thus, a recombinant protein (rBmTI) was
expressed, and was recognized by the anti-histidine and characterized as potential antigenic by bovine serum immunized with the synthetic pepitídeo. MenosThe cattle tick, Rhipicephalus microplus, is the main tick species that undermines productivity losses in livestock resulting in the production of milk and meat throughout Brazil. Its control is primarily chemical and faces problems due to the resistance of ticks to several active principles used. The immune control is a viable alternative to tick control, and the protein BMTI is a strong candidate as a vaccine antigen. The aim of this work was to synthesize the sequencing of BMTI (BmTIS), optimizing it to "codon usage" of Pichia pastoris in an attempt to improve its expression. This sequence was cloned in eukaryotic expression vector pPICZaA, respecting the make reading the AOX1 promoter, present in the plasmid and that is strongly induced by the presence of methanol, following the sign "a-factor for protein secretion, and the tail of poly-histidine. The resulting plasmid, pPICZaBmTIS, X33 was transformed into strains of P. pastoris. Clones were characterized by PCR using primers of the vector and also by dot blot analysis using anti-histidine. A clone (X33BmTIS17) was sequenced and selected to be induced. A single colony clone X33BmTIS17 was inoculated with 25mL of minimal medium with glycerol and incubated at 200rpm, 300C, overnight. The culture was centrifuged at 1500 g, 5min and resuspended in minimal medium with methanol. The supernatant was subjected to SDS-PAGE 0.7%, which showed a major band with the approximate size of 49 kDa. In Western bloting, a band at the same... Mostrar Tudo |
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Thesagro: |
Carrapato; Sanidade Animal. |
|
Thesaurus NAL: |
Rhipicephalus microplus. |
|
Categoria do assunto: |
H Saúde e Patologia
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02512naa a2200217 a 4500
001 1883447
005 2014-09-09
008 2010 bl --- 0-- u #d
100 1 $aANDREOTTI, R.
245 $aHeterologous expression in Pichia pastoris of gene BMTI optimized.
260 $c2010
300 $ap.149-152
500 $aICOPA XII
520 $aThe cattle tick, Rhipicephalus microplus, is the main tick species that undermines productivity losses in livestock resulting in the production of milk and meat throughout Brazil. Its control is primarily chemical and faces problems due to the resistance of ticks to several active principles used. The immune control is a viable alternative to tick control, and the protein BMTI is a strong candidate as a vaccine antigen. The aim of this work was to synthesize the sequencing of BMTI (BmTIS), optimizing it to "codon usage" of Pichia pastoris in an attempt to improve its expression. This sequence was cloned in eukaryotic expression vector pPICZaA, respecting the make reading the AOX1 promoter, present in the plasmid and that is strongly induced by the presence of methanol, following the sign "a-factor for protein secretion, and the tail of poly-histidine. The resulting plasmid, pPICZaBmTIS, X33 was transformed into strains of P. pastoris. Clones were characterized by PCR using primers of the vector and also by dot blot analysis using anti-histidine. A clone (X33BmTIS17) was sequenced and selected to be induced. A single colony clone X33BmTIS17 was inoculated with 25mL of minimal medium with glycerol and incubated at 200rpm, 300C, overnight. The culture was centrifuged at 1500 g, 5min and resuspended in minimal medium with methanol. The supernatant was subjected to SDS-PAGE 0.7%, which showed a major band with the approximate size of 49 kDa. In Western bloting, a band at the same time was recognized by the anti-histidine and by bovine serum immunized with a synthetic based pepitídeo (BmTI). Thus, a recombinant protein (rBmTI) was expressed, and was recognized by the anti-histidine and characterized as potential antigenic by bovine serum immunized with the synthetic pepitídeo.
650 $aRhipicephalus microplus
650 $aCarrapato
650 $aSanidade Animal
700 1 $aSOARES, M.
700 1 $aCUNHA, R. C.
700 1 $aLEITE, F. P. L.
773 $tIn: INTERNATIONAL CONGRESS OF PARASITOLOGY, 12., 2010, Melbourne. Conference abstracts. Melbourne: The Australian Society for Parasitology:World Federation of Parasitologists, 2010.
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