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| Registros recuperados : 317 | |
| 102. |  | ANDREOTTI, R.; GARCIA, M. V.; CUNHA, R. C.; BARROS, J. C. Action of Tagetes minuta (Asteraceae) essential oil in the control of Rhipicephalus microplus (Acari: ixodidae). In: INTERNATIONAL SYMPOSIUM ON TICK CONTROL AND TICK-BORNE DISEASES, 2013, Campo Grande, MS. [Proceedings..]. Campo Grande, MS: Embrapa Gado de Corte, 2013. (Embrapa Gado de Corte. Documentos, 202). Comissão organizadora: Renato Andreotti, Fernando Paiva, Wilson Werner Koller, Jacqueline Cavalcante Barros, Luiz Antonio Dias Leal, Jaqueline Matias.| Biblioteca(s): Embrapa Gado de Corte. |
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| 104. | | OSHIRO, L. M; REIS, F. A.; CAVALCANTE, A. C. R.; SILVA, D. C. da; ANDREOTTI, R. Anticorpos anti-neospora caninum em ovinos no Mato Grosso do Sul. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 17., 2012, São Luis. Parasitologia veterinária, bem estar e produção animal: anais. São Luis: Colégio Brasileiro de Parasitologia Veterinária, 2012. p. 236. Resumo PPR-142.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Corte. |
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| 105. | | MIZIARA, S. R.; PAIVA, F.; KOLLER, W. W.; ANDREOTTI, R.; LOPES, V. A.; PONTES, N. T. Amblyomma humerale koch, 1844 (acarina: ixodidae) parasitando geochelone carbonaria spix, 1824 (chelonia: testudinidae) no estado do mato grosso do sul, brasil CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., 2010, Campo Grande, MS. [Anais...]. Campo Grande, MS: Colégio Brasileiro de Parasitologia Veterinária, 2010. 1 CD-ROM. 1 p.| Biblioteca(s): Embrapa Gado de Corte. |
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| 107. | | AGUIRRE, A. A. R.; GARCIA, M. V.; COSTA, I. N.; MEDEIROS, J. F.; ANDREOTTI, R. Comparação da ixodofauna em peridomicílio nas regiões de amazônia e cerrado do estado de rondônia In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 19; NOVEL APPROACHES TO THE CONTROL OF HELMINTH PARASITES OF LIVESTOCK, 8, 2016, Belém, PA. Anais... Jaboticabal: CBPV, 2016| Biblioteca(s): Embrapa Gado de Corte. |
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| 108. | | ANDREOTTI, R.; PAIVA, F.; LIMA JUNIOR, M. S. C.; CAETANO, A.; MATOS, M. F. C. Clonagem e expressão da porção C-teminal do antígeno de superfície NC-43 de Neospora caninum e sua identidade com anticorpos de bovinos no estado de Mato Grosso do Sul. In: FÓRUM BRASILEIRO DE ESTUDOS SOBRE NEOSPORA CANINUM, 1., 2005, São Paulo, SP. Anais... [São Paulo]: Colégio Brasileiro de Parasitologia Veterinária, 2005. p. 20-21.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 111. | | ANDREOTTI, R.; GARCIA, M. V.; REIS, F. A.; RODRIGUES, V. da S.; BARROS, J. C. Controle de carrapatos em sistemas de produção de bovinos associado ao manejo nutricional no campo. In: ANDREOTTI, R.; GARCIA, M. V.; KOLLER, W. W. (Ed.). Carrapatos na cadeia produtiva de bovinos. Brasília, DF: Embrapa, 2019. 240 p. il. color. p. 181-190| Biblioteca(s): Embrapa Gado de Corte. |
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| 114. | | MATIAS, J. S. 1; ANDREOTTI, R.; BARROS, J. C.; KOLLER, W. W. Levantamento e comercialização de produtos indicados para o controle do carrapato-do-boi rhipicephalus (boophilus) microplus e a mosca-dos-chifres haematobia irritans no município de campo grande, ms. CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., 2010, Campo Grande, MS. [Anais...]. Campo Grande, MS: Colégio Brasileiro de Parasitologia Veterinária, 2010. 1 CD-ROM. 1 p.| Biblioteca(s): Embrapa Gado de Corte. |
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| 116. | | SILVA, B. R. da; GARCIA, M. V.; RODRIGUES, V. da S.; ANDREOTTI, R.; DITTRICH, R. L. Ixodidae fauna of domestic dogs in Parana, southern Brazil. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 26, n. 3, p. 375-377, jul.-set. 2017.| Biblioteca(s): Embrapa Gado de Corte. |
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| 117. | | CUNHA, R. C.; ANDREOTTI, R.; SILVA, E. A. e; ELISÂNGELA PEREIRA; SATO, T.; THOMAZ-SOCCOL, V. Laboratory diagnosis and clinical signs of canine visceral leishmaniasis in dogs examined at the center for zoonosis control in Campo Grande - MS, Brazil. Archives of Veterinary Science, v. 17, n. 4, p. 17-26, 2012. Título em português: Diagnóstico laboratorial e sinais clínicos para Leishmaniose visceral canina em cães examinados no Centro de Controle de Zoonoses de Campo Grande - MS.| Biblioteca(s): Embrapa Gado de Corte. |
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| 119. | | ANDREOTTI, R.; BARROS, J. C.; SILVA, E. A. e; AZZOLINI, S. S.; TANAKA, A. Inibidores de serinoproteases presentes na fase de vida livre do carrapato Rhipicephalus sanguineus. Revista Brasileira de Parasitologia Veterinária, São Paulo, v.13, p. 323, 2004. Suplemento 1, ref. 453. Edição anais do XIII Congresso Brasileiro de Parasitologia Veterinária e I Simpósio Latino-Americano de Rickettsioses, Ouro Preto, MG, set. 2004. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
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| 120. | | GARCIA, M. V.; CUNHA, R. C.; ALMEIDA, R. F. C. de; MATIAS, J.; ANDREOTTI, R. Eficiência de acaricidas em larvas de Rhipicephalus sanguineus, com o uso do teste de pacote de larvas. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 17., 2012, São Luis. Parasitologia veterinária, bem estar e produção animal: anais. São Luis: Colégio Brasileiro de Parasitologia Veterinária, 2012. p. 157 PA 017| Biblioteca(s): Embrapa Gado de Corte. |
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| Registros recuperados : 317 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
22/03/2011 |
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Data da última atualização: |
09/09/2014 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
ANDREOTTI, R.; SOARES, M.; CUNHA, R. C.; LEITE, F. P. L. |
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Afiliação: |
RENATO ANDREOTTI E SILVA, CNPGC; UFPEL; UFPEL; UFPEL. |
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Título: |
Heterologous expression in Pichia pastoris of gene BMTI optimized. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONGRESS OF PARASITOLOGY, 12., 2010, Melbourne. Conference abstracts. Melbourne: The Australian Society for Parasitology:World Federation of Parasitologists, 2010. |
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Páginas: |
p.149-152 |
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Idioma: |
Inglês
Português |
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Notas: |
ICOPA XII |
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Conteúdo: |
The cattle tick, Rhipicephalus microplus, is the main tick species that undermines productivity losses in livestock resulting in the production of milk and meat throughout Brazil. Its control is primarily chemical and faces problems due to the resistance of ticks to several active principles used. The immune control is a viable alternative to tick control, and the protein BMTI is a strong candidate as a vaccine antigen. The aim of this work was to synthesize the sequencing of BMTI (BmTIS), optimizing it to "codon usage" of Pichia pastoris in an attempt to improve its expression. This sequence was cloned in eukaryotic expression vector pPICZaA, respecting the make reading the AOX1 promoter, present in the plasmid and that is strongly induced by the presence of methanol, following the sign "a-factor for protein secretion, and the tail of poly-histidine. The resulting plasmid, pPICZaBmTIS, X33 was transformed into strains of P. pastoris. Clones were characterized by PCR using primers of the vector and also by dot blot analysis using anti-histidine. A clone (X33BmTIS17) was sequenced and selected to be induced. A single colony clone X33BmTIS17 was inoculated with 25mL of minimal medium with glycerol and incubated at 200rpm, 300C, overnight. The culture was centrifuged at 1500 g, 5min and resuspended in minimal medium with methanol. The supernatant was subjected to SDS-PAGE 0.7%, which showed a major band with the approximate size of 49 kDa. In Western bloting, a band at the same time was recognized by the anti-histidine and by bovine serum immunized with a synthetic based pepitídeo (BmTI). Thus, a recombinant protein (rBmTI) was
expressed, and was recognized by the anti-histidine and characterized as potential antigenic by bovine serum immunized with the synthetic pepitídeo. MenosThe cattle tick, Rhipicephalus microplus, is the main tick species that undermines productivity losses in livestock resulting in the production of milk and meat throughout Brazil. Its control is primarily chemical and faces problems due to the resistance of ticks to several active principles used. The immune control is a viable alternative to tick control, and the protein BMTI is a strong candidate as a vaccine antigen. The aim of this work was to synthesize the sequencing of BMTI (BmTIS), optimizing it to "codon usage" of Pichia pastoris in an attempt to improve its expression. This sequence was cloned in eukaryotic expression vector pPICZaA, respecting the make reading the AOX1 promoter, present in the plasmid and that is strongly induced by the presence of methanol, following the sign "a-factor for protein secretion, and the tail of poly-histidine. The resulting plasmid, pPICZaBmTIS, X33 was transformed into strains of P. pastoris. Clones were characterized by PCR using primers of the vector and also by dot blot analysis using anti-histidine. A clone (X33BmTIS17) was sequenced and selected to be induced. A single colony clone X33BmTIS17 was inoculated with 25mL of minimal medium with glycerol and incubated at 200rpm, 300C, overnight. The culture was centrifuged at 1500 g, 5min and resuspended in minimal medium with methanol. The supernatant was subjected to SDS-PAGE 0.7%, which showed a major band with the approximate size of 49 kDa. In Western bloting, a band at the same... Mostrar Tudo |
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Thesagro: |
Carrapato; Sanidade Animal. |
|
Thesaurus NAL: |
Rhipicephalus microplus. |
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Categoria do assunto: |
H Saúde e Patologia
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02512naa a2200217 a 4500
001 1883447
005 2014-09-09
008 2010 bl --- 0-- u #d
100 1 $aANDREOTTI, R.
245 $aHeterologous expression in Pichia pastoris of gene BMTI optimized.
260 $c2010
300 $ap.149-152
500 $aICOPA XII
520 $aThe cattle tick, Rhipicephalus microplus, is the main tick species that undermines productivity losses in livestock resulting in the production of milk and meat throughout Brazil. Its control is primarily chemical and faces problems due to the resistance of ticks to several active principles used. The immune control is a viable alternative to tick control, and the protein BMTI is a strong candidate as a vaccine antigen. The aim of this work was to synthesize the sequencing of BMTI (BmTIS), optimizing it to "codon usage" of Pichia pastoris in an attempt to improve its expression. This sequence was cloned in eukaryotic expression vector pPICZaA, respecting the make reading the AOX1 promoter, present in the plasmid and that is strongly induced by the presence of methanol, following the sign "a-factor for protein secretion, and the tail of poly-histidine. The resulting plasmid, pPICZaBmTIS, X33 was transformed into strains of P. pastoris. Clones were characterized by PCR using primers of the vector and also by dot blot analysis using anti-histidine. A clone (X33BmTIS17) was sequenced and selected to be induced. A single colony clone X33BmTIS17 was inoculated with 25mL of minimal medium with glycerol and incubated at 200rpm, 300C, overnight. The culture was centrifuged at 1500 g, 5min and resuspended in minimal medium with methanol. The supernatant was subjected to SDS-PAGE 0.7%, which showed a major band with the approximate size of 49 kDa. In Western bloting, a band at the same time was recognized by the anti-histidine and by bovine serum immunized with a synthetic based pepitídeo (BmTI). Thus, a recombinant protein (rBmTI) was expressed, and was recognized by the anti-histidine and characterized as potential antigenic by bovine serum immunized with the synthetic pepitídeo.
650 $aRhipicephalus microplus
650 $aCarrapato
650 $aSanidade Animal
700 1 $aSOARES, M.
700 1 $aCUNHA, R. C.
700 1 $aLEITE, F. P. L.
773 $tIn: INTERNATIONAL CONGRESS OF PARASITOLOGY, 12., 2010, Melbourne. Conference abstracts. Melbourne: The Australian Society for Parasitology:World Federation of Parasitologists, 2010.
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