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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
28/07/2016 |
Data da última atualização: |
09/08/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
TORRES, E. B.; NÓBREGA, R. S. A.; FERNANDES JUNIOR, P. I.; SILVA, L. B.; CARVALHO, G. dos S.; MARINHO, R. de C. N.; PAVAN, B. E. |
Afiliação: |
ELIDA BARROS TORRES, Federal University of Piauí, Campus Professora Cinobelina Elvas, Bom Jesus, Piauí; RAFAELA S. A. NÓBREGA, Agricultural, Environmental and Biological Sciences Center, Federal University of Recôncavo da Bahia, Cruz das Almas, Bahia; PAULO IVAN FERNANDES JUNIOR, CPATSA; LUCIANA BARBOZA SILVA, Crop Sciences Department, Federal University of Piauí, Campus Professora Cinobelina Elvas, Bom Jesus, Piauí; GABRIEL DOS SANTOS CARVALHO, Crop Sciences Department, Federal University of Piauí, Campus Professora Cinobelina Elvas, Bom Jesus, Piauí; RITA DE CASSIA NUNES MARINHO, Crop Sciences Department, Federal University of Piauí, Campus Professora Cinobelina Elvas, Bom Jesus, Piauí; BRUNO E. PAVAN, UNESP, Campus de Ilha Solteira, Sao Paulo. |
Título: |
The damage caused by Callosobruchus maculatus on cowpea grains is dependent on the plant genotype. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Journal of the Science of Food and Agriculture, v. 96, n. 12, p. 4276-4280, 2016. |
DOI: |
10.1002/jsfa.7639 |
Idioma: |
Inglês |
Conteúdo: |
Beans from cowpea cultivars fertilized with mineral N or inoculated with various rhizobium strainsmay contain different nitrogen concentrations and nitrogen metabolite composition, which affects the beans? defense mechanisms against pests. In this study, the population growth of Callosobruchus maculatus reared on beans from four cowpea cultivars fertilized with different nitrogen sources was evaluated. The factors tested were beans from four cowpea cultivars and seven different nitrogen sources: mineral N fertilization, inoculation with five strains of symbiotic diazotrophic bacteria, and soil nitrogen (absolute control). |
Palavras-Chave: |
Adubação nitrogenada; Cowpea; Feijão caupi; Grãos armazenados; Inoculation; Nitrogen fertilization; Rizóbio. |
Thesagro: |
Adubação; Callosobruchus Maculatus; Genótipo; Inoculação; Rhizobium. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/145868/1/Paulo-Ivan-2.pdf
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Marc: |
LEADER 01668naa a2200349 a 4500 001 2049761 005 2017-08-09 008 2016 bl uuuu u00u1 u #d 024 7 $a10.1002/jsfa.7639$2DOI 100 1 $aTORRES, E. B. 245 $aThe damage caused by Callosobruchus maculatus on cowpea grains is dependent on the plant genotype.$h[electronic resource] 260 $c2016 520 $aBeans from cowpea cultivars fertilized with mineral N or inoculated with various rhizobium strainsmay contain different nitrogen concentrations and nitrogen metabolite composition, which affects the beans? defense mechanisms against pests. In this study, the population growth of Callosobruchus maculatus reared on beans from four cowpea cultivars fertilized with different nitrogen sources was evaluated. The factors tested were beans from four cowpea cultivars and seven different nitrogen sources: mineral N fertilization, inoculation with five strains of symbiotic diazotrophic bacteria, and soil nitrogen (absolute control). 650 $aAdubação 650 $aCallosobruchus Maculatus 650 $aGenótipo 650 $aInoculação 650 $aRhizobium 653 $aAdubação nitrogenada 653 $aCowpea 653 $aFeijão caupi 653 $aGrãos armazenados 653 $aInoculation 653 $aNitrogen fertilization 653 $aRizóbio 700 1 $aNÓBREGA, R. S. A. 700 1 $aFERNANDES JUNIOR, P. I. 700 1 $aSILVA, L. B. 700 1 $aCARVALHO, G. dos S. 700 1 $aMARINHO, R. de C. N. 700 1 $aPAVAN, B. E. 773 $tJournal of the Science of Food and Agriculture$gv. 96, n. 12, p. 4276-4280, 2016.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
29/03/2016 |
Data da última atualização: |
28/03/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
AGUIRRE, A. de A. R.; LOBO, F. P.; ANDREOTTI, R. |
Afiliação: |
ANDRÉ DE ABREU RANGEL AGUIRRE, FAMEZ/UFMS; FRANCISCO PEREIRA LOBO, CNPTIA; RENATO ANDREOTTI E SILVA, CNPGC. |
Título: |
Design of the ATAQ peptide and its evaluation as an immunogen to develop a Rhipicephalus vaccine. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Veterinary Parasitology, v. 221, p. 30-38, 2016 |
Idioma: |
Inglês |
Conteúdo: |
Tick infestation may cause several problems including affecting domestic animal health and reducing the production of meat and milk, among others. Resistance to several classes of acaricides have been reported, forcing researchers to search for alternative measures, such as vaccines against ticks, to ensure tick control while having no or at least low negative impacts on the environment and public health. However, the current commercially available vaccines in different strains of Rhipicephalus microplus are reported to be of low efficacy. Fortunately, reverse vaccinology approaches have shown positive results in the new generation of vaccines. On this basis, a synthetic peptide from the ATAQ protein, which is present in the gut and Malpighi tubes of R. microplus, was synthesized. The ATAQ proteins were isolated, characterized and sequenced from several species of the genus Rhipicephalus. The alignment showed 93.3% identity among DNA sequences of ATAQs from these species. Because of this, immunization trials with this peptide were conducted on mice, rabbits and cattle to evaluate the humoral immune response and the efficacy against Rhipicephalus sanguineus in addition to R. microplus. Based on recent results, we conclude that reverse vaccinology is a promising approach because it is more accurate and faster than conventional methods in the detection of potential antigens to use in anti-tick vaccines. It is not only applicable against R. microplus but also against tick species that play important roles in spreading other diseases. ATAQ proteins should be considered as the antigen in new trials to develop a multi-antigenic vaccine. Although these peptides behave as hapten and are not able to be recognized by the immune system on its own, using carriers and adjuvants helps its presentation and induces strong immune responses. Furthermore, an efficiency of 35% reduction in overall life cycle parameters was reported for R. microplus (98% for ELISA responder animals) and 47% for R. sanguineus. Although not yet enough to prevent the environment to infestation of ticks, this still constitutes a promising strategy that could be applied to integrated measures on tick control and in new research that develops anti-tick vaccines. MenosTick infestation may cause several problems including affecting domestic animal health and reducing the production of meat and milk, among others. Resistance to several classes of acaricides have been reported, forcing researchers to search for alternative measures, such as vaccines against ticks, to ensure tick control while having no or at least low negative impacts on the environment and public health. However, the current commercially available vaccines in different strains of Rhipicephalus microplus are reported to be of low efficacy. Fortunately, reverse vaccinology approaches have shown positive results in the new generation of vaccines. On this basis, a synthetic peptide from the ATAQ protein, which is present in the gut and Malpighi tubes of R. microplus, was synthesized. The ATAQ proteins were isolated, characterized and sequenced from several species of the genus Rhipicephalus. The alignment showed 93.3% identity among DNA sequences of ATAQs from these species. Because of this, immunization trials with this peptide were conducted on mice, rabbits and cattle to evaluate the humoral immune response and the efficacy against Rhipicephalus sanguineus in addition to R. microplus. Based on recent results, we conclude that reverse vaccinology is a promising approach because it is more accurate and faster than conventional methods in the detection of potential antigens to use in anti-tick vaccines. It is not only applicable against R. microplus but also against tick specie... Mostrar Tudo |
Palavras-Chave: |
ATAQ; Reverse vaccinology; Synthetic peptide. |
Thesaurus NAL: |
Immunization; Rhipicephalus microplus; Rhipicephalus sanguineus. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/158239/1/Design-of-the-ATAQ-peptide-and-its-evaluation.pdf
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Marc: |
LEADER 02914naa a2200217 a 4500 001 2041997 005 2017-03-28 008 2016 bl uuuu u00u1 u #d 100 1 $aAGUIRRE, A. de A. R. 245 $aDesign of the ATAQ peptide and its evaluation as an immunogen to develop a Rhipicephalus vaccine.$h[electronic resource] 260 $c2016 520 $aTick infestation may cause several problems including affecting domestic animal health and reducing the production of meat and milk, among others. Resistance to several classes of acaricides have been reported, forcing researchers to search for alternative measures, such as vaccines against ticks, to ensure tick control while having no or at least low negative impacts on the environment and public health. However, the current commercially available vaccines in different strains of Rhipicephalus microplus are reported to be of low efficacy. Fortunately, reverse vaccinology approaches have shown positive results in the new generation of vaccines. On this basis, a synthetic peptide from the ATAQ protein, which is present in the gut and Malpighi tubes of R. microplus, was synthesized. The ATAQ proteins were isolated, characterized and sequenced from several species of the genus Rhipicephalus. The alignment showed 93.3% identity among DNA sequences of ATAQs from these species. Because of this, immunization trials with this peptide were conducted on mice, rabbits and cattle to evaluate the humoral immune response and the efficacy against Rhipicephalus sanguineus in addition to R. microplus. Based on recent results, we conclude that reverse vaccinology is a promising approach because it is more accurate and faster than conventional methods in the detection of potential antigens to use in anti-tick vaccines. It is not only applicable against R. microplus but also against tick species that play important roles in spreading other diseases. ATAQ proteins should be considered as the antigen in new trials to develop a multi-antigenic vaccine. Although these peptides behave as hapten and are not able to be recognized by the immune system on its own, using carriers and adjuvants helps its presentation and induces strong immune responses. Furthermore, an efficiency of 35% reduction in overall life cycle parameters was reported for R. microplus (98% for ELISA responder animals) and 47% for R. sanguineus. Although not yet enough to prevent the environment to infestation of ticks, this still constitutes a promising strategy that could be applied to integrated measures on tick control and in new research that develops anti-tick vaccines. 650 $aImmunization 650 $aRhipicephalus microplus 650 $aRhipicephalus sanguineus 653 $aATAQ 653 $aReverse vaccinology 653 $aSynthetic peptide 700 1 $aLOBO, F. P. 700 1 $aANDREOTTI, R. 773 $tVeterinary Parasitology$gv. 221, p. 30-38, 2016
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