Data/hora: 19/06/2024 19:49:04

Biblioteca(s): 	Embrapa Soja.	
Data corrente: 	07/04/2004	
Data da última atualização: 	21/08/2009	
Autoria: 	ANDREOLI, C.	
Título: 	Bioassay method for identifying and detecting genetically modified soybean.	
Ano de publicação: 	2004	
Fonte/Imprenta: 	In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004.	
Páginas: 	p. 252.	
Série: 	(Embrapa Soja. Documentos, 228).	
Idioma: 	Inglês	
Notas: 	Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi.	
Conteúdo: 	There are several commomly used GMO testing protocols, including biological tests, as well as ELISA and PCR tests, for herbicide and insect tolerance. Growers and end-users should consider the advantages and disadvantages of the various testing methods before harvest. Exporters should probably resign themselves to the most rigorous testing protocol, to anticipate the additional scrutiny their products will receive overseas. Large food processors are currently using a combination of tests for identity-preserved (IP) grains. The herbicide bioassays could be used to detect GMO herbicide tolerant traits in Roundup Ready and Liberty Link soybeans and corn. The current test involves placing seeds in a germination media, moistened with a diluted solution containing the herbicide, or spraying the herbicide on seedlings. Seeds that test positive for the presence of the herbicide-tolerant GMO trait will germinate and develop normally, whereas those that die or not develop normally will be GMO-free. Because it is relatively inexpensive, this procedure is widely used by seed producers and grain companies exporting soybeans in the USA. Therefore, the main goal of this research was to test a modified herbicide bioassay for soybean tolerant to glyphosate. Four hundred seeds of conventional soybean, cv. BRS 133 and BRS 214 and RR soybean, cv. BRS Baliza, were imbibed in a solution of 400 ppm glyphosate (i.a = 360gL-1) for 6 hours in the laboratory room. After treatment, seeds were germinated in a paper rolls with four replications at 25º C, for 3 days. The seedlings were classified as a normal, abnormal and susceptible to the herbicide. The trials were replicated six times in order to validate the protocol. The susceptible seedlings presented reduced growth and no secondary roots and the abnormal seedlings are due to low vigor. The RR soybean germinate 96% against 0% (zero) for conventional soybeans. The test showed significant differences between conventional and RR soybean, facilitating the distinction among treatments. The great advantage of this modified protocol was that results could be obtained in three days against six days as it was proposed before. Besides the low cost and a quicker test, the herbicide bioassay seems to be a promising test for detecting GM soybean by seed producers, end-users and exporters. Researchers are being carried out at Embrapa Soja in order to reduce the test time for one day.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Soja (CNPSO)	
Exemplares: 	CNPSO - (23755-1) - UMT - PL - -- - RF 633.34072 - W927a -  - DIS