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| Registros recuperados : 15 | |
| 5. |  | ÂNGELO, F. F.; ARCURI, E. F.; ANDRADE, N. J.; MERHI, C. M.; LANGE, C. C. Avaliação da presença de genes para enterotoxinas, pela técnica de PCR multiplex, em Staphylococcus aureus isolados de mastite bovina na Zona da Mata mineira. In: CONGRESSO NACIONAL DE LATICÍNIOS, 24., 2007, Juiz de Fora. Anais... Belo Horizonte: EPAMIG, 2007. p. 410-413.| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | ÂNGELO, F. F.; CARVALHO, A. F.; ARCURI, E. F.; ANDRADE, N. J.; MERHI, C. M. Ocorrência de Staphylococcus aureus em leite de tanques individuais na bacia leiteira de Viçosa, Minas Gerais. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 24., 2007, Brasília. Resumos... São Paulo: SBM, 2007.| Biblioteca(s): Embrapa Gado de Leite. |
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| 11. | | BASTOS, M. do S. R.; SOARES, N. de F.; ANDRADE, N. J. de; ALVES, R. E.; ARRUDA, A. C. Efeito da associação de sanitização e surfactante na redução de microbiota da superfície de melão cantaloupe. Horticultura Brasileira, Brasília, v. 21, n. 2, jul. 2003. Suplemento 2. Trabalho apresentado no 43º Congresso Brasileiro de Olericultura, 2003. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 21, n. 2, p. 387, jul. 2003. Suplemento 1.| Biblioteca(s): Embrapa Hortaliças. |
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| 12. | | SILVA, F. da; SANTOS, R. H. S.; ANDRADE, N. J. de; BARBOSA, L. C. A.; CASALI, V. W. D.; LIMA, R. R. de; PASSARINHO, R. V. de M. Basil conservation affected by cropping season, harvest time and storage period. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 40, n. 4, p. 323-328, abr. 2005 Título em português: Armazenamento e conservação de manjericão após diferentes épocas e horários de colheita.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 13. | | KAMEYAMA, O.; ABRÃO JÚNIOR, J.; TEIXEIRA, J. M. de A.; ANDRADE, N. J.; MININ, V. P. R.; SOARES, L. dos S. Extrato de própolis na sanitização e conservação de cenoura minimamente processada. Revista Ceres, Viçosa, v. 55, n. 3, p. 218-223, jun. 2008.| Biblioteca(s): Embrapa Hortaliças. |
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| 14. | | ÂNGELO, F. F.; ARCURI, E. F.; TALON, R.; BORGES, M. de F.; LEROY, S.; LOISEAU, G.; LANGE, C. C.; ANDRADE, N. J. de; MONTET, D. Toxigenic status of Staphylococcus aureus isolated from bovine raw milk and minas frescal cheese in Brazil. Journal of Food Protection, Des Moines, v. 73, n. 12, p. 2225?2231, 2010.| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 15. | | ARCURI, E. F.; ÂNGELO, F. F.; TALON, R.; BORGES, M. de F.; LEROY, S.; LOISEAU, G.; LANGE, C. C.; ANDRADE, N. J. de; MONTET, D. Toxigenic status of Staphylococcus aureus isolated from bovine raw milk and minas frescal cheese in Brazil. Journal of Food Protection, v. 73, n. 12, p. 2225-2231, 2010.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 15 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
18/03/2011 |
|
Data da última atualização: |
09/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
ARCURI, E. F.; ÂNGELO, F. F.; TALON, R.; BORGES, M. de F.; LEROY, S.; LOISEAU, G.; LANGE, C. C.; ANDRADE, N. J. de; MONTET, D. |
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Afiliação: |
EDNA FROEDER ARCURI, CNPGL; FABÍOLA FONSECA ÂNGELO, UFV; RÉGINE TALON, Institut National de la Recherche Agronomique; MARIA DE FATIMA BORGES, CNPAT; SABINE LEROY, Institut National de la Recherche Agronomique; GÉRARD LOISEAU, Centre de Coopération Internationale en Recherche Agronomique pour le Dévelopment; CARLA CHRISTINE LANGE, CNPGL; NÉLIO JOSÉ DE ANDRADE, UFV; DIDIER MONTET, Céntre de Coopération Internationale en Recherche Agronomique pour le Dévelopment. |
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Título: |
Toxigenic status of Staphylococcus aureus isolated from bovine raw milk and minas frescal cheese in Brazil. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Journal of Food Protection, v. 73, n. 12, p. 2225-2231, 2010. |
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DOI: |
https://doi.org/10.4315/0362-028X-73.12.2225 |
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Idioma: |
Inglês |
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Conteúdo: |
A group of 291 Staphylococcus aureus isolates from mastitic cow's milk (n = 125), bulk tank milk (n = 96), and Minas frescal cheese (n = 70) were screened for staphylococcal enterotoxin (SE) genes (sea, seb, sec, sed, see, seg, seh, sei, selj, and sell) and for the tst-1 gene encoding staphylococcal toxic shock syndrome toxin 1 by PCR assay. A total of 109 (37.5%) of the isolates were positive for at least one of these 11 genes, and 23 distinct genotypes of toxin genes were observed. Of the S. aureus isolates bearing SE genes, 17 (13.6%) were from mastitic cow's milk, 41 (41.7%) were from bulk tank milk, and 51 (72.9%) were from Minas frescal cheese. The occurrence of exclusively more recently described SE genes (seg through sell) was considerably higher (87 of 109 PCR-positive strains) than that of classical SE genes (sea through see, 15 strains). The SE genes most commonly detected were seg and sei; they were found alone or in different combinations with other toxin genes, but in 60.8% of the cases they were codetected. No strain possessed see. The tst-1 gene was found in eight isolates but none from mastitic cow's milk. Macrorestriction analysis of chromosomal DNA from 89 S. aureus isolates positive for SE gene(s) was conducted with the enzyme SmaI. Fifty-five distinct pulsed-field gel electrophoresis patterns were found, demonstrating a lack of predominance of any specific clone. A second enzyme, ApaI, used for some isolates was less discriminating than SmaI. The high genotype diversity of potential toxigenic S. aureus strains found in this study, especially from Minas frescal cheese, suggests various sources of contamination. Efforts from the entire production chain are required to improve consumer safety. MenosA group of 291 Staphylococcus aureus isolates from mastitic cow's milk (n = 125), bulk tank milk (n = 96), and Minas frescal cheese (n = 70) were screened for staphylococcal enterotoxin (SE) genes (sea, seb, sec, sed, see, seg, seh, sei, selj, and sell) and for the tst-1 gene encoding staphylococcal toxic shock syndrome toxin 1 by PCR assay. A total of 109 (37.5%) of the isolates were positive for at least one of these 11 genes, and 23 distinct genotypes of toxin genes were observed. Of the S. aureus isolates bearing SE genes, 17 (13.6%) were from mastitic cow's milk, 41 (41.7%) were from bulk tank milk, and 51 (72.9%) were from Minas frescal cheese. The occurrence of exclusively more recently described SE genes (seg through sell) was considerably higher (87 of 109 PCR-positive strains) than that of classical SE genes (sea through see, 15 strains). The SE genes most commonly detected were seg and sei; they were found alone or in different combinations with other toxin genes, but in 60.8% of the cases they were codetected. No strain possessed see. The tst-1 gene was found in eight isolates but none from mastitic cow's milk. Macrorestriction analysis of chromosomal DNA from 89 S. aureus isolates positive for SE gene(s) was conducted with the enzyme SmaI. Fifty-five distinct pulsed-field gel electrophoresis patterns were found, demonstrating a lack of predominance of any specific clone. A second enzyme, ApaI, used for some isolates was less discriminating than SmaI. The high ge... Mostrar Tudo |
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Palavras-Chave: |
Minas frescal cheese. |
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Thesagro: |
Staphylococcus Aureus. |
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Categoria do assunto: |
Q Alimentos e Nutrição Humana |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/881434/1/Toxigenic-status-of-Staphylococcus-aureus-isolated-from-bovine.pdf
|
|
Marc: |
LEADER 02530naa a2200253 a 4500
001 1881434
005 2022-08-09
008 2010 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.4315/0362-028X-73.12.2225$2DOI
100 1 $aARCURI, E. F.
245 $aToxigenic status of Staphylococcus aureus isolated from bovine raw milk and minas frescal cheese in Brazil.$h[electronic resource]
260 $c2010
520 $aA group of 291 Staphylococcus aureus isolates from mastitic cow's milk (n = 125), bulk tank milk (n = 96), and Minas frescal cheese (n = 70) were screened for staphylococcal enterotoxin (SE) genes (sea, seb, sec, sed, see, seg, seh, sei, selj, and sell) and for the tst-1 gene encoding staphylococcal toxic shock syndrome toxin 1 by PCR assay. A total of 109 (37.5%) of the isolates were positive for at least one of these 11 genes, and 23 distinct genotypes of toxin genes were observed. Of the S. aureus isolates bearing SE genes, 17 (13.6%) were from mastitic cow's milk, 41 (41.7%) were from bulk tank milk, and 51 (72.9%) were from Minas frescal cheese. The occurrence of exclusively more recently described SE genes (seg through sell) was considerably higher (87 of 109 PCR-positive strains) than that of classical SE genes (sea through see, 15 strains). The SE genes most commonly detected were seg and sei; they were found alone or in different combinations with other toxin genes, but in 60.8% of the cases they were codetected. No strain possessed see. The tst-1 gene was found in eight isolates but none from mastitic cow's milk. Macrorestriction analysis of chromosomal DNA from 89 S. aureus isolates positive for SE gene(s) was conducted with the enzyme SmaI. Fifty-five distinct pulsed-field gel electrophoresis patterns were found, demonstrating a lack of predominance of any specific clone. A second enzyme, ApaI, used for some isolates was less discriminating than SmaI. The high genotype diversity of potential toxigenic S. aureus strains found in this study, especially from Minas frescal cheese, suggests various sources of contamination. Efforts from the entire production chain are required to improve consumer safety.
650 $aStaphylococcus Aureus
653 $aMinas frescal cheese
700 1 $aÂNGELO, F. F.
700 1 $aTALON, R.
700 1 $aBORGES, M. de F.
700 1 $aLEROY, S.
700 1 $aLOISEAU, G.
700 1 $aLANGE, C. C.
700 1 $aANDRADE, N. J. de
700 1 $aMONTET, D.
773 $tJournal of Food Protection$gv. 73, n. 12, p. 2225-2231, 2010.
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