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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
16/09/2014 |
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Data da última atualização: |
13/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PAULA, D. P.; ANDOW, D. A.; TIMBÓ, R. V.; SUJII, E. R.; PIRES, C. S. S. |
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Afiliação: |
DEBORA PIRES PAULA, CENARGEN; DAVID A. ANDOW, UNIVERSITY OF MINNESOTA, USA; RENATA VELOZO TIMBÓ, UNB; EDISON RYOITI SUJII, CENARGEN; CARMEN SILVIA SOARES PIRES, CENARGEN. |
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Título: |
Uptake and transfer of a Bt toxin by a lepidoptera to Its eggs and effects on Its offspring. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
PLoS ONE, v. 9, n. 4, 2014. (Open access). |
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Idioma: |
Inglês |
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Conteúdo: |
Research on non-target effects of transgenic crop plants has focused primarily on bitrophic, tritrophic and indirect effects of entomotoxins from Bacillus thuringiensis, but little work has considered intergenerational transfer of Cry proteins. This work reports a lepidopteran (Chlosyne lacinia) taking up a Bt entomotoxin when exposed to sublethal or low concentrations, transferring the entomotoxin to eggs, and having adverse effects on the first filial generation (F1) offspring. Two bioassays were conducted using a sublethal concentration of toxin (100.0 ng/ml Cry1Ac) for adults and a concentration equal to the LC10 (2.0 ng/ml Cry1Ac) for larvae. Cry1Ac is the most common entomotoxin expressed in Bt cotton in Brazil. In the adult diet bioassay there was no adverse effect on the parental generation (P0) adults, but the F1 larvae had higher mortality and longer development time compared to F1 larvae of parents that did not ingest Cry1Ac. For the 3rd instar larvae, there was no measurable effect on the P0 larvae, pupae and adults, but the F1 larvae had higher mortality and longer development time. Using chemiluminescent Western Blot, Cry1Ac was detected in F1 eggs laid by P0 butterflies from both bioassays. Our study indicates that, at least for this species and these experimental conditions, a ,65 kDa insecticidal protein can be taken up and transferred to descendants where it can increase mortality and development time. |
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Thesaurus Nal: |
Lepidoptera. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/180016/1/pone.0095422.pdf
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Marc: |
LEADER 01971naa a2200181 a 4500
001 1995058
005 2023-03-13
008 2014 bl uuuu u00u1 u #d
100 1 $aPAULA, D. P.
245 $aUptake and transfer of a Bt toxin by a lepidoptera to Its eggs and effects on Its offspring.$h[electronic resource]
260 $c2014
520 $aResearch on non-target effects of transgenic crop plants has focused primarily on bitrophic, tritrophic and indirect effects of entomotoxins from Bacillus thuringiensis, but little work has considered intergenerational transfer of Cry proteins. This work reports a lepidopteran (Chlosyne lacinia) taking up a Bt entomotoxin when exposed to sublethal or low concentrations, transferring the entomotoxin to eggs, and having adverse effects on the first filial generation (F1) offspring. Two bioassays were conducted using a sublethal concentration of toxin (100.0 ng/ml Cry1Ac) for adults and a concentration equal to the LC10 (2.0 ng/ml Cry1Ac) for larvae. Cry1Ac is the most common entomotoxin expressed in Bt cotton in Brazil. In the adult diet bioassay there was no adverse effect on the parental generation (P0) adults, but the F1 larvae had higher mortality and longer development time compared to F1 larvae of parents that did not ingest Cry1Ac. For the 3rd instar larvae, there was no measurable effect on the P0 larvae, pupae and adults, but the F1 larvae had higher mortality and longer development time. Using chemiluminescent Western Blot, Cry1Ac was detected in F1 eggs laid by P0 butterflies from both bioassays. Our study indicates that, at least for this species and these experimental conditions, a ,65 kDa insecticidal protein can be taken up and transferred to descendants where it can increase mortality and development time.
650 $aLepidoptera
700 1 $aANDOW, D. A.
700 1 $aTIMBÓ, R. V.
700 1 $aSUJII, E. R.
700 1 $aPIRES, C. S. S.
773 $tPLoS ONE$gv. 9, n. 4, 2014. (Open access).
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
09/01/2013 |
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Data da última atualização: |
25/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 3 |
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Autoria: |
SILVA, S. X. de B.; BARBOSA, F. F. L.; ANDRADE, E. C. de; SOARES, A. C. F.; ALMEIDA, D. de O. |
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Afiliação: |
SUELY XAVIER DE BRITO SILVA, ADAB; FRANCISCO FERRAZ LARANJEIRA BARBOSA, CNPMF; EDUARDO CHUMBINHO DE ANDRADE, SRI; ANA CRISTINA FERMINO SOARES, UFRB; DÉCIO DE OLIVEIRA ALMEIDA, UFRB. |
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Título: |
Minimum sample size, prevalence and incidence of Brevipalpus phoenicis (Geijskes) (Acari: Tenuipalpidae) in Recôncavo Baiano. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Magistra, Cruz das Almas-BA, v. 24, n.o esp., p. 17-25, dez. 2012. |
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ISSN: |
0102-5333 |
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Idioma: |
Inglês |
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Notas: |
Semana Entomológica da Bahia (SINSECTA), 03 a 09 de dezembro de 2012. |
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Conteúdo: |
The Recôncavo is responsible for almost 20% of citrus production in the state of Bahia, Brazil, where the occurrence of the Citrus leprosis virus (CiLV) has been recorded, one of the most important viruses in citrus cultivation, and whose vector is the Tenuipalpidae mite (Brevipalpus phoenicis). This study is to define the minimum sample size to quantify the prevalence, incidence and spatial distribution of the mite vector in the Recôncavo Baiano (RB). Two pilot samplings were made in 145 orchards located in 13 municipalities, in April and September 2008, at the end of the drought and rainy period, respectively. In each orchard, three fruits per plant were inspected using a pocket magnifying glass (magnification10x), with a total of 21 plants/orchard, recording the presence or absence of the mite vector. Maximum prevalence (100%) of the mite was found in RB in both samplings. To estimate the incidence of infestation in plants, a minimum of 18 orchards should be sampled, and the minimum sample size was 89 orchards to estimate the infestation in fruits. Infestation of Brevipalpus was verified in 74% and 63% of the plants and in 41% and 34% of the fruits in April and September, respectively. The dispersion index (DI) was about 2.59 and 4.72, classifying the spatial distribution of orchards infested by B. phoenicis in the agro-ecological conditions of the RB as aggregated. No symptom of CiLV was detected, characterizing the RB as an area free of this virus. |
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Palavras-Chave: |
Fruta cíttrica; Recôncavo Baiano. |
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Thesagro: |
Brevipalpus Phoenicis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02275naa a2200229 a 4500
001 1944561
005 2023-05-25
008 2012 bl uuuu u00u1 u #d
022 $a0102-5333
100 1 $aSILVA, S. X. de B.
245 $aMinimum sample size, prevalence and incidence of Brevipalpus phoenicis (Geijskes) (Acari$bTenuipalpidae) in Recôncavo Baiano.$h[electronic resource]
260 $c2012
500 $aSemana Entomológica da Bahia (SINSECTA), 03 a 09 de dezembro de 2012.
520 $aThe Recôncavo is responsible for almost 20% of citrus production in the state of Bahia, Brazil, where the occurrence of the Citrus leprosis virus (CiLV) has been recorded, one of the most important viruses in citrus cultivation, and whose vector is the Tenuipalpidae mite (Brevipalpus phoenicis). This study is to define the minimum sample size to quantify the prevalence, incidence and spatial distribution of the mite vector in the Recôncavo Baiano (RB). Two pilot samplings were made in 145 orchards located in 13 municipalities, in April and September 2008, at the end of the drought and rainy period, respectively. In each orchard, three fruits per plant were inspected using a pocket magnifying glass (magnification10x), with a total of 21 plants/orchard, recording the presence or absence of the mite vector. Maximum prevalence (100%) of the mite was found in RB in both samplings. To estimate the incidence of infestation in plants, a minimum of 18 orchards should be sampled, and the minimum sample size was 89 orchards to estimate the infestation in fruits. Infestation of Brevipalpus was verified in 74% and 63% of the plants and in 41% and 34% of the fruits in April and September, respectively. The dispersion index (DI) was about 2.59 and 4.72, classifying the spatial distribution of orchards infested by B. phoenicis in the agro-ecological conditions of the RB as aggregated. No symptom of CiLV was detected, characterizing the RB as an area free of this virus.
650 $aBrevipalpus Phoenicis
653 $aFruta cíttrica
653 $aRecôncavo Baiano
700 1 $aBARBOSA, F. F. L.
700 1 $aANDRADE, E. C. de
700 1 $aSOARES, A. C. F.
700 1 $aALMEIDA, D. de O.
773 $tMagistra, Cruz das Almas-BA$gv. 24, n.o esp., p. 17-25, dez. 2012.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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