| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Corte. |
|
Data corrente: |
04/08/2012 |
|
Data da última atualização: |
04/08/2012 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso |
|
Autoria: |
ALMEIDA, R. G. de; COSTA, J. A. A. da; BEHLING NETO, A.; ZIMMER, A. H.; KICHEL, A. N.; MACEDO, M. C. M. |
|
Afiliação: |
ROBERTO GIOLO DE ALMEIDA, CNPGC; JOSE ALEXANDRE AGIOVA DA COSTA, CNPC; Arthur Behling Neto, Doutorando em Agricultura Tropical da UFMT, Cuiabá, MT.; ADEMIR HUGO ZIMMER, CNPGC; ARMINDO NEIVO KICHEL, CNPGC; MANUEL CLAUDIO MOTTA MACEDO, CNPGC. |
|
Título: |
Taxas de semeadura para Brachiaria brizantha em consórcio com sorgo de corte e pastejo na safrinha. |
|
Ano de publicação: |
2012 |
|
Fonte/Imprenta: |
In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 49., 2012, Brasília. A produção animal no mundo em transformação: anais. Brasília, DF: SBZ, 2012. |
|
Páginas: |
3 p. |
|
Descrição Física: |
1 CD-ROM. |
|
Idioma: |
Português |
|
Notas: |
Trabalho 4SPF. |
|
Conteúdo: |
O experimento foi realizado na Embrapa Gado de Corte, Campo Grande, MS, com objetivo de avaliar
taxas de semeadura para Brachiaria brizantha em consórcio com sorgo de corte e pastejo (Sorghum spp. cv. BRS
802) na safrinha. Foi adotado delineamento em blocos completos casualizados, em esquema de parcelas
subdivididas com um tratamento adicional (sorgo) e quatro repetições. Os tratamentos da parcela corresponderam a
um fatorial 2x4, sendo dois capins (B. brizantha cv. BRS Piatã e B. brizantha acesso B6) e quatro taxas de
semeadura (3, 6, 9 e 12 kg/ha de sementes puras viáveis, SPV). Os tratamentos da subparcela corresponderam a
dois sistemas de cultivo dos capins (monocultivo e consórcio com sorgo). As forrageiras foram semeadas em
fevereiro de 2011 e avaliadas após 60 dias. No estabelecimento, o capim-B6 apresenta maior produtividade e
cobertura do solo do que o capim-piatã. A taxa de semeadura promove efeito linear crescente sobre a produtividade
do capim, altura do capim e cobertura do solo, e efeito linear decrescente sobre a razão folha: caule do capim e a
produtividade de invasoras. A taxa de semeadura de 7,88 kg/ha de SPV de B. brizantha promove maior
produtividade do sorgo (3.206 kg/ha), aos 60 dias após semeadura das forrageiras. |
|
Palavras-Chave: |
Consorciação; Piatã; Sorghum spp. cv. BRS 802. |
|
Thesagro: |
Brachiaria Brizantha; Pastagem; Sorgo. |
|
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
|
Marc: |
LEADER 02172naa a2200277 a 4500
001 1930444
005 2012-08-04
008 2012 bl uuuu u00u1 u #d
100 1 $aALMEIDA, R. G. de
245 $aTaxas de semeadura para Brachiaria brizantha em consórcio com sorgo de corte e pastejo na safrinha.
260 $c2012
300 $a3 p.$c1 CD-ROM.
500 $aTrabalho 4SPF.
520 $aO experimento foi realizado na Embrapa Gado de Corte, Campo Grande, MS, com objetivo de avaliar taxas de semeadura para Brachiaria brizantha em consórcio com sorgo de corte e pastejo (Sorghum spp. cv. BRS 802) na safrinha. Foi adotado delineamento em blocos completos casualizados, em esquema de parcelas subdivididas com um tratamento adicional (sorgo) e quatro repetições. Os tratamentos da parcela corresponderam a um fatorial 2x4, sendo dois capins (B. brizantha cv. BRS Piatã e B. brizantha acesso B6) e quatro taxas de semeadura (3, 6, 9 e 12 kg/ha de sementes puras viáveis, SPV). Os tratamentos da subparcela corresponderam a dois sistemas de cultivo dos capins (monocultivo e consórcio com sorgo). As forrageiras foram semeadas em fevereiro de 2011 e avaliadas após 60 dias. No estabelecimento, o capim-B6 apresenta maior produtividade e cobertura do solo do que o capim-piatã. A taxa de semeadura promove efeito linear crescente sobre a produtividade do capim, altura do capim e cobertura do solo, e efeito linear decrescente sobre a razão folha: caule do capim e a produtividade de invasoras. A taxa de semeadura de 7,88 kg/ha de SPV de B. brizantha promove maior produtividade do sorgo (3.206 kg/ha), aos 60 dias após semeadura das forrageiras.
650 $aBrachiaria Brizantha
650 $aPastagem
650 $aSorgo
653 $aConsorciação
653 $aPiatã
653 $aSorghum spp. cv. BRS 802
700 1 $aCOSTA, J. A. A. da
700 1 $aBEHLING NETO, A.
700 1 $aZIMMER, A. H.
700 1 $aKICHEL, A. N.
700 1 $aMACEDO, M. C. M.
773 $tIn: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 49., 2012, Brasília. A produção animal no mundo em transformação: anais. Brasília, DF: SBZ, 2012.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Corte (CNPGC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
10/02/2012 |
|
Data da última atualização: |
27/02/2023 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
ABREU, E. F. M.; ANDRADE, E. C. de; ARAGAO, F. J. L. |
|
Afiliação: |
Emanuel Felipe Medereiros Abreu, UnB; EDUARDO CHUMBINHO DE ANDRADE, CNPMF; FRANCISCO JOSE LIMA ARAGAO, CENARGEN. |
|
Título: |
Molecular comparison between isolates of Cowpea severe mosaic virus and Cowpea aphid borne mosaic virus from Northeast Brazil. |
|
Ano de publicação: |
2011 |
|
Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. pdf 34699 |
|
Páginas: |
p. 6 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Cowpea (Vigna unguiculata) is an important plant crop in Northeast Brazil being traditionally cultivated by small farmers. Virus diseases are considered to be the main factor limiting cowpea yield in the region. The severe mosaic disease caused by the Cowpea severe mosaic virus (CpSMV), family Comoviridae, genus Comovirus, seems to be one of the most prevalent diseases leading to high yield losses in this crop. The CABMV belongs to the genus Potyvirus in the Potyviridae family, and infects cowpea worldwide. In the northeastern Region of Brazil, both viruses can be found in cowpea planted areas. The aim of the present study was to access the degree of homology among a 2500 and 1500bp region of different isolates of CpSMV and CABMV, respectively; obtained in different northeastern regions in Brazil, and to compare it to isolates throughout the world. Plants with CPSMV and CABMV symptoms from the states of Piauí, Ceará, Rio Grande do Norte, Paraiba, Pernambuco, Alagoas, Sergipe and Bahia were collected, and the isolates were identified by RT-PCR analysis. Total RNA was extracted from infected tissue with Trizol Reagent (Invitrogen) according to manufacturer's recommendations, and afterwards used for synthesis of cDNA fragments by RT-PCR using the Superscript® III Reverse Polymerase and Taq DNA Polymerase (Invitrogen), according to manufacturer?s recommendations. The synthesized primers were able to amplify fragments of 2500 and 1500bp of the CpSMV and CABMV virus, respectively, by RT-PCR. Amplification products were directly cloned into the pGEMT-Easy plasmid vector (Promega), according to the manufacturer?s instructions. Cloned fragments were sequenced in both orientations. Deduced amino acid sequences of the virus were compared to sequences available from GenBank. Multiple sequence alignments were obtained with Clustal W. Phylogenetic trees using the MEGA version 4.1 software package and the neighbour-joining method with Poisson correction. Tree branches were bootstrapped with 1000 permutations. CpSMV and CABMV diseases remain as limiting factors in this crop in Brazil, and breeding programs, either by conventional or engineered approaches, should be targeted at establishing resistance of cowpeas to CpSMV and CABMV. MenosCowpea (Vigna unguiculata) is an important plant crop in Northeast Brazil being traditionally cultivated by small farmers. Virus diseases are considered to be the main factor limiting cowpea yield in the region. The severe mosaic disease caused by the Cowpea severe mosaic virus (CpSMV), family Comoviridae, genus Comovirus, seems to be one of the most prevalent diseases leading to high yield losses in this crop. The CABMV belongs to the genus Potyvirus in the Potyviridae family, and infects cowpea worldwide. In the northeastern Region of Brazil, both viruses can be found in cowpea planted areas. The aim of the present study was to access the degree of homology among a 2500 and 1500bp region of different isolates of CpSMV and CABMV, respectively; obtained in different northeastern regions in Brazil, and to compare it to isolates throughout the world. Plants with CPSMV and CABMV symptoms from the states of Piauí, Ceará, Rio Grande do Norte, Paraiba, Pernambuco, Alagoas, Sergipe and Bahia were collected, and the isolates were identified by RT-PCR analysis. Total RNA was extracted from infected tissue with Trizol Reagent (Invitrogen) according to manufacturer's recommendations, and afterwards used for synthesis of cDNA fragments by RT-PCR using the Superscript® III Reverse Polymerase and Taq DNA Polymerase (Invitrogen), according to manufacturer?s recommendations. The synthesized primers were able to amplify fragments of 2500 and 1500bp of the CpSMV and CABMV virus, respectively,... Mostrar Tudo |
|
Palavras-Chave: |
CABMV; Cowpea; CpSMV; Molecular and RT-PCR analysis; Virus diseases. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
LEADER 02995nam a2200205 a 4500
001 1915005
005 2023-02-27
008 2011 bl uuuu u01u1 u #d
100 1 $aABREU, E. F. M.
245 $aMolecular comparison between isolates of Cowpea severe mosaic virus and Cowpea aphid borne mosaic virus from Northeast Brazil.$h[electronic resource]
260 $aIn: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3, 2011, Ilhéus. Resumos. [S. l.]: Sociedade Brasileira de Genética, 2011. pdf 34699$c2011
300 $ap. 6
520 $aCowpea (Vigna unguiculata) is an important plant crop in Northeast Brazil being traditionally cultivated by small farmers. Virus diseases are considered to be the main factor limiting cowpea yield in the region. The severe mosaic disease caused by the Cowpea severe mosaic virus (CpSMV), family Comoviridae, genus Comovirus, seems to be one of the most prevalent diseases leading to high yield losses in this crop. The CABMV belongs to the genus Potyvirus in the Potyviridae family, and infects cowpea worldwide. In the northeastern Region of Brazil, both viruses can be found in cowpea planted areas. The aim of the present study was to access the degree of homology among a 2500 and 1500bp region of different isolates of CpSMV and CABMV, respectively; obtained in different northeastern regions in Brazil, and to compare it to isolates throughout the world. Plants with CPSMV and CABMV symptoms from the states of Piauí, Ceará, Rio Grande do Norte, Paraiba, Pernambuco, Alagoas, Sergipe and Bahia were collected, and the isolates were identified by RT-PCR analysis. Total RNA was extracted from infected tissue with Trizol Reagent (Invitrogen) according to manufacturer's recommendations, and afterwards used for synthesis of cDNA fragments by RT-PCR using the Superscript® III Reverse Polymerase and Taq DNA Polymerase (Invitrogen), according to manufacturer?s recommendations. The synthesized primers were able to amplify fragments of 2500 and 1500bp of the CpSMV and CABMV virus, respectively, by RT-PCR. Amplification products were directly cloned into the pGEMT-Easy plasmid vector (Promega), according to the manufacturer?s instructions. Cloned fragments were sequenced in both orientations. Deduced amino acid sequences of the virus were compared to sequences available from GenBank. Multiple sequence alignments were obtained with Clustal W. Phylogenetic trees using the MEGA version 4.1 software package and the neighbour-joining method with Poisson correction. Tree branches were bootstrapped with 1000 permutations. CpSMV and CABMV diseases remain as limiting factors in this crop in Brazil, and breeding programs, either by conventional or engineered approaches, should be targeted at establishing resistance of cowpeas to CpSMV and CABMV.
653 $aCABMV
653 $aCowpea
653 $aCpSMV
653 $aMolecular and RT-PCR analysis
653 $aVirus diseases
700 1 $aANDRADE, E. C. de
700 1 $aARAGAO, F. J. L.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
