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| 21. |  | PEREIRA, Á. J.; ALFENAS-ZERBINI, P.; CASCARDO, R. S.; ANDRADE, E. C. de; ZERBINI, F. M. Analysis of the full-length genome sequence of papaya lethal yellowing virus (PLYV), determined by deep sequencing, confirms its classification in the genus Sobemovirus. Archives of Virology, v. 157, p. 2009-2011, 2012.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 26. | | CERQUEIRA, L. R. de S.; ABREU, E. F. M.; ANDRADE, E. C. de. Desenvolvimento de métodos diagnósticos para os vírus causadores da murcha do abacaxizeiro (PMWAV -1, 2 e 3). In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA, 8., 2014, Cruz das Almas, Ba. Pesquisa: despertando mentes para a inovação e transformando o futuro :[anais]. Cruz das Almas, Ba, Embrapa Mandioca e Fruticultura, 2014.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 29. | | BARROS, A. M. de; DELIZA, R.; ANDRADE, E. C. B. de; FARIAS, A. X. de. Desenvolvimento do perfil sensorial de leite pasteurizado tipo B. In: SEMANA DE DEBATES CIENTÍFICOS, 15.; FEIRA DE EXTENSÃO, 5.; ENCONTRO DE EXTENSÃO, 8., 2002, Rio de Janeiro. Resumos... Rio de Janeiro: UNIRIO, 2002. 1 CD-Rom.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 36. | | CALEGARIO, R. F.; ANDRADE, E. C.; FERREIRA, S. S.; MANHANI, G. G.; ZERBINI, F. M. Biological and molecular characterization of a tomato isolate of Sidra micrantha mosaic vírus (SimMV). Fitopatologia Brasileira, Brasília, DF, v. 30, p. S180, ago. 2006. Suplemento. Resumo 749. Trabalho apresentado no 38. Congresso Brasileiro de Fitopatologia, 2005, Brasília, DF.| Biblioteca(s): Embrapa Hortaliças. |
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| 39. | | CALEGARIO, R. F.; ALFENAS, P. F.; COTRIM, M. A.; ANDRADE, E. C.; ZERBINI, F. M. Caracterization of a new tomato-infecting begomovirus and its potencial use a vector for transient gene expression in plants. Virus Reviews & Research, Florianópolis, v. 8, p. 193-194, 2003. Resumo. Trabalho apresentado no 14º National Meeting of Virology, Florianópolis, 2003.| Biblioteca(s): Embrapa Hortaliças. |
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| 40. | | CALEGARIO, R. F.; FERREIRA, S. de S.; ANDRADE, E. C. de; ZERBINI, F. M. Characterization of Tomato yellow spot virus, a novel tomato-infecting begomovirus in Brazil Pesquisa Agropecuária Brasileira, Brasília, DF, v. 42, n. 9, p. 1335-1343, set. 2007 Título em português: Caracterização do Tomato yellow spot virus, um novo begomovírus isolado de tomateiro no Brasil.| Biblioteca(s): Embrapa Hortaliças; Embrapa Unidades Centrais. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura; Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
20/02/2013 |
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Data da última atualização: |
19/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ABREU, E. F. M.; TINOCO, M. L. P.; ANDRADE, E. C. de; ARAGAO, F. J. L. |
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Afiliação: |
EMANUEL FELIPE MEDEIROS ABREU, CNPMF; MARIA LAINE PENHA TINOCO, CENARGEN; EDUARDO CHUMBINHO DE ANDRADE, SRI; FRANCISCO JOSE LIMA ARAGAO, CENARGEN. |
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Título: |
Diversity among isolates of cowpea severe mosaic virus infecting cowpeas in northeastern Brazil. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 11, n. 3, p. 3146-3153, 2012. |
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ISSN: |
1676-5680 |
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Idioma: |
Inglês |
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Conteúdo: |
Eleven isolates of cowpea severe mosaic virus (CPSMV), a member of the genus Comovirus, were selected from 50 samples collected of nine cowpea fields in Northeastern Brazil (Piauí, Ceará, Rio Grande do Norte, Paraíba, Pernambuco, Alagoas, Sergipe, Bahia, and Distrito Federal) and partially sequenced. The RNA1 partial sequence, corresponding to the helicase, viral genome-linked protein, picornain 3C-like protease, and the RNA-directed RNA polymerase genes from CPSMV, had high identity among isolates, varying from 98 to 100%. No evidence was found for intermolecular or intramolecular recombination. Phylogenetic analysis confirmed that the Brazilian CPSMV isolates are substantially different from the CPSMV strain USA. Despite the low variability found among Brazilian CPSMV isolates, there were notable differences in the symptomatology of infected cowpea plants, ranging from mild to moderate. Previous reports have demonstrated an association between CPSMV symptom determinants and helicase. However, we found no correlation between the helicase Diversity among isolates of cowpea severe mosaic virus 3147 mutations and symptoms caused by CPSMV. Nevertheless, all isolates with mutation R to K in the protease provoked severe symptoms. This type of information can provide a foundation for the development of strategies to produce durable resistant cowpea lines. It is crucial for strategies based on DNA sequence-dependent technologies, such as inhibition with RNAi. |
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Palavras-Chave: |
Isolate of cowpea. |
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Thesaurus NAL: |
Cowpea severe mosaic virus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02092naa a2200193 a 4500
001 1950162
005 2023-06-19
008 2012 bl uuuu u00u1 u #d
022 $a1676-5680
100 1 $aABREU, E. F. M.
245 $aDiversity among isolates of cowpea severe mosaic virus infecting cowpeas in northeastern Brazil.$h[electronic resource]
260 $c2012
520 $aEleven isolates of cowpea severe mosaic virus (CPSMV), a member of the genus Comovirus, were selected from 50 samples collected of nine cowpea fields in Northeastern Brazil (Piauí, Ceará, Rio Grande do Norte, Paraíba, Pernambuco, Alagoas, Sergipe, Bahia, and Distrito Federal) and partially sequenced. The RNA1 partial sequence, corresponding to the helicase, viral genome-linked protein, picornain 3C-like protease, and the RNA-directed RNA polymerase genes from CPSMV, had high identity among isolates, varying from 98 to 100%. No evidence was found for intermolecular or intramolecular recombination. Phylogenetic analysis confirmed that the Brazilian CPSMV isolates are substantially different from the CPSMV strain USA. Despite the low variability found among Brazilian CPSMV isolates, there were notable differences in the symptomatology of infected cowpea plants, ranging from mild to moderate. Previous reports have demonstrated an association between CPSMV symptom determinants and helicase. However, we found no correlation between the helicase Diversity among isolates of cowpea severe mosaic virus 3147 mutations and symptoms caused by CPSMV. Nevertheless, all isolates with mutation R to K in the protease provoked severe symptoms. This type of information can provide a foundation for the development of strategies to produce durable resistant cowpea lines. It is crucial for strategies based on DNA sequence-dependent technologies, such as inhibition with RNAi.
650 $aCowpea severe mosaic virus
653 $aIsolate of cowpea
700 1 $aTINOCO, M. L. P.
700 1 $aANDRADE, E. C. de
700 1 $aARAGAO, F. J. L.
773 $tGenetics and Molecular Research$gv. 11, n. 3, p. 3146-3153, 2012.
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