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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
06/07/1998 |
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Data da última atualização: |
28/03/2017 |
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Autoria: |
RICE; C. D.; KERGOSIEN, D. H.; ADAMS, S. M. |
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Título: |
Innate immune function as a bioindicator of pollution stress in fish. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Ecotoxicology and Environmental Safety, v.33, n.2, p.186-192, 1996. |
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Idioma: |
Inglês |
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Conteúdo: |
Immunotoxicological studies, bnased on processing of samples in the field and laboratory, were conducted on fish collected from a stream receiving point-source contaminants near its headwaters. Previous studies in this stream have revealed that cytochrome P4501A activity, liver somatic indices, macrophage aggregates, and parasitic liver lesion are significantly elevated in sunfish with the degree of impact decreasing with distance from the contaminant source. Fish collected from each sampling site were equally divided, One group was sacrificed in the field and the spleen and anterior kidney tissues were removed and placed in buffer on ice. The other group was kept in MS-222 fopr 2 hr and transported to the laboratory for processing. The spleen and anterior kidney from each fish were then prepared as a single cell suspension and shipped overnight to Mississippi State University. Cells were then evaluated for PMA-stimulated phagocyte oxidative burst and non-specific cytotoxic cell (NCC) activity against K562 tumor targets. Oxidative burst responses were drmatically suppressed in both groups at sampling sites near the headwaters but returned to reference further downstream. There were no differences between processing strategies at each station. NCC activities did not follow gradient-response patterns observed with phagocyte oxidative burst data and there were inconsistent differences between processing strategies at each site. These data indicate that simple immune function assays, such as phagocyte oxidative burst responses, can be used as a ancillary bioindicator in fish health monitoring and that immune function in these fish can be reliably assessed even if samples are not immediately processed. MenosImmunotoxicological studies, bnased on processing of samples in the field and laboratory, were conducted on fish collected from a stream receiving point-source contaminants near its headwaters. Previous studies in this stream have revealed that cytochrome P4501A activity, liver somatic indices, macrophage aggregates, and parasitic liver lesion are significantly elevated in sunfish with the degree of impact decreasing with distance from the contaminant source. Fish collected from each sampling site were equally divided, One group was sacrificed in the field and the spleen and anterior kidney tissues were removed and placed in buffer on ice. The other group was kept in MS-222 fopr 2 hr and transported to the laboratory for processing. The spleen and anterior kidney from each fish were then prepared as a single cell suspension and shipped overnight to Mississippi State University. Cells were then evaluated for PMA-stimulated phagocyte oxidative burst and non-specific cytotoxic cell (NCC) activity against K562 tumor targets. Oxidative burst responses were drmatically suppressed in both groups at sampling sites near the headwaters but returned to reference further downstream. There were no differences between processing strategies at each station. NCC activities did not follow gradient-response patterns observed with phagocyte oxidative burst data and there were inconsistent differences between processing strategies at each site. These data indicate that simple immune function a... Mostrar Tudo |
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Palavras-Chave: |
Bioindicador; Bioindicator; Funcao imunologica; Immune function. |
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Thesagro: |
Peixe; Poluição. |
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Thesaurus Nal: |
fish; pollution. |
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Categoria do assunto: |
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Marc: |
LEADER 02383naa a2200253 a 4500
001 1792737
005 2017-03-28
008 1996 bl --- 0-- u #d
100 1 $aRICE
245 $aInnate immune function as a bioindicator of pollution stress in fish.
260 $c1996
520 $aImmunotoxicological studies, bnased on processing of samples in the field and laboratory, were conducted on fish collected from a stream receiving point-source contaminants near its headwaters. Previous studies in this stream have revealed that cytochrome P4501A activity, liver somatic indices, macrophage aggregates, and parasitic liver lesion are significantly elevated in sunfish with the degree of impact decreasing with distance from the contaminant source. Fish collected from each sampling site were equally divided, One group was sacrificed in the field and the spleen and anterior kidney tissues were removed and placed in buffer on ice. The other group was kept in MS-222 fopr 2 hr and transported to the laboratory for processing. The spleen and anterior kidney from each fish were then prepared as a single cell suspension and shipped overnight to Mississippi State University. Cells were then evaluated for PMA-stimulated phagocyte oxidative burst and non-specific cytotoxic cell (NCC) activity against K562 tumor targets. Oxidative burst responses were drmatically suppressed in both groups at sampling sites near the headwaters but returned to reference further downstream. There were no differences between processing strategies at each station. NCC activities did not follow gradient-response patterns observed with phagocyte oxidative burst data and there were inconsistent differences between processing strategies at each site. These data indicate that simple immune function assays, such as phagocyte oxidative burst responses, can be used as a ancillary bioindicator in fish health monitoring and that immune function in these fish can be reliably assessed even if samples are not immediately processed.
650 $afish
650 $apollution
650 $aPeixe
650 $aPoluição
653 $aBioindicador
653 $aBioindicator
653 $aFuncao imunologica
653 $aImmune function
700 1 $aC. D.
700 1 $aKERGOSIEN, D. H.
700 1 $aADAMS, S. M.
773 $tEcotoxicology and Environmental Safety$gv.33, n.2, p.186-192, 1996.
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Registro original: |
Embrapa Pantanal (CPAP) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
13/02/2004 |
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Data da última atualização: |
29/10/2018 |
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Autoria: |
DONATO, V. M. T. S.; ANDRADE, A. G. de; SOUZA, E. S. de; FRANÇA, J. G. E. de. |
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Afiliação: |
Virgínia Maria Tenorio Sabino Donato; Arnóbio Gonçalves de Andrade, Universidade Federal Rural de Pernambuco - UFRPE/Departamento de Química Vegetal; Eidy Simões de Souza, Empresa Pernambucana de Pesquisa Agropecuária; José Geraldo Eugênio de França, Empresa Pernambucana de Pesquisa Agropecuária. |
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Título: |
Metabolismo de plantas de cana-de-açúcar cultivadas in vitro sob diferentes concentrações de nitrogênio. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 38, n. 12, p. 1373-1379, dez. 2003 |
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Idioma: |
Português |
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Notas: |
Título em inglês: Metabolism of sugar cane plant cultivated in vitro at different nitrogen levels. |
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Conteúdo: |
Variedades de cana-de-açúcar (RB 732577, RB 83102, RB 75126, CB 45-3, Caiana e Co 997) foram cultivadas in vitro em meio nutritivo básico MS com diferentes concentrações de N (9,83 mM, 7,37 mM, 4,92 mM, 2,46 mM, 0,49 mM e 0,0 mM), com o objetivo de avaliar a eficiência no processo de assimilação desse elemento. Após 45 dias de cultivo, foram determinados os ganhos de biomassa e os teores de amônio, aminoácidos e açúcares solúveis. A variedade Co 997 apresentou maior ganho de biomassa e maior quantidade de N incorporado em aminoácidos livres em relação às demais variedades, sugerindo maior eficiência no processo de assimilação. Os teores mais elevados de amônio foram observados nas variedades RB 83102 e RB 75126, quando cultivadas em meio desprovido de N, mostrando-se menos eficientes no processo de assimilação uma vez que apresentaram os menores valores de aminoácidos livres nas mesmas condições de cultivo. |
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Palavras-Chave: |
fixação do nitrogênio. |
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Thesagro: |
Aminoácido; Biomassa. |
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Thesaurus NAL: |
amino acids; biomass; nitrogen fixation; Saccharum. |
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Categoria do assunto: |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/108449/1/Metabolismo.pdf
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Marc: |
LEADER 01799naa a2200253 a 4500
001 1109683
005 2018-10-29
008 2003 bl uuuu u00u1 u #d
100 1 $aDONATO, V. M. T. S.
245 $aMetabolismo de plantas de cana-de-açúcar cultivadas in vitro sob diferentes concentrações de nitrogênio.
260 $c2003
500 $aTítulo em inglês: Metabolism of sugar cane plant cultivated in vitro at different nitrogen levels.
520 $aVariedades de cana-de-açúcar (RB 732577, RB 83102, RB 75126, CB 45-3, Caiana e Co 997) foram cultivadas in vitro em meio nutritivo básico MS com diferentes concentrações de N (9,83 mM, 7,37 mM, 4,92 mM, 2,46 mM, 0,49 mM e 0,0 mM), com o objetivo de avaliar a eficiência no processo de assimilação desse elemento. Após 45 dias de cultivo, foram determinados os ganhos de biomassa e os teores de amônio, aminoácidos e açúcares solúveis. A variedade Co 997 apresentou maior ganho de biomassa e maior quantidade de N incorporado em aminoácidos livres em relação às demais variedades, sugerindo maior eficiência no processo de assimilação. Os teores mais elevados de amônio foram observados nas variedades RB 83102 e RB 75126, quando cultivadas em meio desprovido de N, mostrando-se menos eficientes no processo de assimilação uma vez que apresentaram os menores valores de aminoácidos livres nas mesmas condições de cultivo.
650 $aamino acids
650 $abiomass
650 $anitrogen fixation
650 $aSaccharum
650 $aAminoácido
650 $aBiomassa
653 $afixação do nitrogênio
700 1 $aANDRADE, A. G. de
700 1 $aSOUZA, E. S. de
700 1 $aFRANÇA, J. G. E. de
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 38, n. 12, p. 1373-1379, dez. 2003
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Embrapa Unidades Centrais (AI-SEDE) |
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