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4. | | BOA SORTE, P. M. F.; TEIXEIRA, K. R. dos S.; REIS, V. M.; BALDANI, J. I. Use of DGGE to aussess the endophytic diversity of two seguracane varieties inoculated with endophytic diazotroph bacteria. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. Sll. 09 Biblioteca(s): Embrapa Agrobiologia. |
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8. | | MELO, L. H. V.; BALDANI, J. I.; GITAHY, P. M.; BOA SORTE, P. M. F.; AVELAR, J. P. avaliação da capacidade endofítica de bacillus thuringiensis em plantas de Cana-de-açúcar In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 25.,2009, Porto de Galinhas, PE. Anais... [São Paulo]: Sociedade Bras. de Microbiologia, 2009. 01 01 de 01 Biblioteca(s): Embrapa Agrobiologia. |
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10. | | NDAW, S. M.; BOA SORTE, P. M. F.; BALDANI, J. I.; CANELLAS, L. P.; OLIVARES, F. L. Combining effect of the heat tratament and herbaspirillum seropedicae inoculation over sugarcane associated bacteria diversity using DGGE approach. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. SV.20 Biblioteca(s): Embrapa Agrobiologia. |
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13. | | BOA SORTE, P. M. F.; FERREIRA, J. S.; OLIVEIRA, A. L. M.; TEIXEIRA, K. R. dos S.; REIS, V. M.; BALDANI, J. I. Análise da comunidade de bactérias endofíticas em plantas de cana-de-açúcar após quatro ciclos de cultivo. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 28., REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 12., SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 10., REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 7., 2008, Londrina. FertBio 2008: desafios para o uso do solo com eficiência e qualidade ambiental: anais. Londrina: Embrapa Soja: SBCS: IAPAR, UEL, 2008. 4 p. 1 CD-ROM. Parceria: UFRRJ; Universidade Estadual de Londrina.
Organizado por: Adilson de Oliveira Júnior, Regina Maria Villas Bôas de Campos Leite, César de Castro, Fábio Álvares de Oliveira; Odilon Ferreira Saraiva. Biblioteca(s): Embrapa Agrobiologia. |
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14. | | OLIVEIRA, A. L. M.; BOA SORTE, P. M. F.; CANUTO, E. L.; KENNEDY, C.; BODDEY, R. M.; REIS, V. M.; BALDANI, J. I. Inoculação de estirpes selvagens e mutante NIF- de Gluconacetobacter diazotrophicus em plantas micropropagadas de cana-de-açúcar. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 30., 2005, Recife. Solos: sustentabilidade e qualidade ambiental. Recife: Sociedade Brasileira de Ciência do Solo, 2005. 4 p. CD ROM Biblioteca(s): Embrapa Agrobiologia. |
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15. | | BOA SORTE, P. M. F.; ARAUJO, J. L. S. de; MELO, L. V. de; GALISA, P. de S.; LEAL, L.; BALDANI, J. I.; BALDANI, V. L. D. Develpment of a real-time PCR assay for the detection and quantification on Gluconacetobacter diazotrophicus in sugarcane grown under field conditions. African Journal of Microbiology Research, v. 8, n. 31, p. 2937-2946, july, 2014 Biblioteca(s): Embrapa Agrobiologia. |
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Registros recuperados : 16 | |
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Registro Completo
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
01/03/2011 |
Data da última atualização: |
04/03/2011 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
MARRACINI, P.; RAMOS, H. J. O.; VIEIRA, L. G. E.; FERRAO, M. A. G.; SILVA, F. R. da; MELO, J. A. T.; BLOCH JUNIOR, C.; ANDRADE, A. C. |
Afiliação: |
IAPAR; IAPAR; MARIA AMELIA GAVA FERRAO, SAPC; JORGE ALEX TAQUITA MELO, CENARGEN; CARLOS BLOCH JUNIOR, CENARGEN; ALAN CARVALHO ANDRADE, CENARGEN. |
Título: |
Study of drought-tolerance mechanisms in coffee plants by an integrated analysis. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas. |
Idioma: |
Inglês |
Conteúdo: |
The principal aim of this study was to investigate the molecular mechanisms underlying the response to drought stress in coffee plants by different approaches. In order to indentify candidate genes involved in controlling drought tolerance in coffee plants, different strategies were followed in our laboratories. The first used the nucleic data generated by the Brazilian Coffee EST project to identify candidate genes (CG) by in silico analysis (electronic Northern-blot). Differential expression of these CG was verified in leaves and roots from drought-tolerant and susceptible clones of C. canephora var. Conilon by Northern-blot and quantitative PCR experiments. The second was based on the screening of macroarray membranes spotted with coffee ESTs which were hybridized separately with leaf cDNA probes of the same clones. Finally, 2D gel electrophoresis was also performed to selected proteins presenting differential accumulation in leaves of the same clones. These proteins were analyzed by MALDI-TOF-MS/MS leading to the identification of a new set of CG. Results concerning the identification of CG by these different approaches are presented and discussed. |
Thesagro: |
Clone; Coffea Canephora. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01831nam a2200217 a 4500 001 1880084 005 2011-03-04 008 2008 bl uuuu u00u1 u #d 100 1 $aMARRACINI, P. 245 $aStudy of drought-tolerance mechanisms in coffee plants by an integrated analysis.$h[electronic resource] 260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas.$c2008 520 $aThe principal aim of this study was to investigate the molecular mechanisms underlying the response to drought stress in coffee plants by different approaches. In order to indentify candidate genes involved in controlling drought tolerance in coffee plants, different strategies were followed in our laboratories. The first used the nucleic data generated by the Brazilian Coffee EST project to identify candidate genes (CG) by in silico analysis (electronic Northern-blot). Differential expression of these CG was verified in leaves and roots from drought-tolerant and susceptible clones of C. canephora var. Conilon by Northern-blot and quantitative PCR experiments. The second was based on the screening of macroarray membranes spotted with coffee ESTs which were hybridized separately with leaf cDNA probes of the same clones. Finally, 2D gel electrophoresis was also performed to selected proteins presenting differential accumulation in leaves of the same clones. These proteins were analyzed by MALDI-TOF-MS/MS leading to the identification of a new set of CG. Results concerning the identification of CG by these different approaches are presented and discussed. 650 $aClone 650 $aCoffea Canephora 700 1 $aRAMOS, H. J. O. 700 1 $aVIEIRA, L. G. E. 700 1 $aFERRAO, M. A. G. 700 1 $aSILVA, F. R. da 700 1 $aMELO, J. A. T. 700 1 $aBLOCH JUNIOR, C. 700 1 $aANDRADE, A. C.
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