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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
08/12/2011 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, I. O. e; VASCONCELOS, R. B.; CAETANO, J. V. O.; GULART, L. V. M.; CAMARGO, L. S. de A.; BÁO, S. N.; SILVA, A. A. M. R. e. |
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Afiliação: |
I. OLIVEIRA E SILVA, UNB; R. B. VASCONCELOS, UNB; J. V. O. CAETANO, UNB; L. V. M. GULART, UNB; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; S. N. BÁO, UNB; A. A. M. ROSA E SILVA, UNB. |
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Título: |
Induction of reversible meiosis arrest of bovine oocytes using a two-step procedure under defined and nondefined conditions. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Theriogenology, v. 75, n. 6, p. 1115-1124, 2011. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2010.11.021 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective was to study the effect of a defined culture system, on nuclear and cytoplasmic maturation of bovine oocytes, using the two-step procedure of IVM to detect possible inhibition and subsequent resumption of meiosis arrest. In the first step, called the prematuration period (PMP), COCs were cultured in T1-non-defined medium (NDM), or T2-defined medium (DM), both for 24 h. In step 2, called the resumption period (RP), COCs were cultured in: NDM (T1); DM + NDM (T3); or DM+DM (T4) for 24 h in each medium. The NDM was composed of TCM-199 supplemented with FCS and FSH. The DM was composed of alpha-MEM supplemented with PVA, insulin, IGF-1, androstenedione, nonessential amino acids, transferrin, and sodium selenium. Oocytes from T2 had a lower (P < 0.05) rate of nuclear maturation (19.8%) than T1 oocytes (83.2%). Also, T2 COCs appeared to be in the process of cytoplasmic maturation, according to the distribution of organelles assessed by transmission electron microscopy (MET). These COCs had characteristics previously described as mature: erect microvilli on the plasmembrane, presence of cortical/evenly distributed mitochondria throughout the ooplasm, and presence of 50% aligned/cluster cortical granules. Immature characteristics such as small PvS, compact cumulus cells, and presence of 50% cortical granule clusters were also observed. The T1 COCs had only characteristics of maturation (P < 0.05). In step 2 (RP), meiosis arrest induced by DM was resumed after an additional 24 h of culture in NDM (T3) with 79.2% mature COCs, whereas in T4, meiosis arrest was maintained, resulting in almost 70% immature COCs (P < 0.05). At the end of RP, T3 COCs had the mature characteristics of mitochondria spread throughout the cytoplasm (P < 0.05), cumulus expansion, and alignment of cortical granules, whereas the T4 group had both immature and mature characteristics. We inferred that DM can be used in lieu of meiosis inhibitors and furthermore, it can provide extra time to study nuclear and cytoplasmic maturation synchrony of IVM. MenosThe objective was to study the effect of a defined culture system, on nuclear and cytoplasmic maturation of bovine oocytes, using the two-step procedure of IVM to detect possible inhibition and subsequent resumption of meiosis arrest. In the first step, called the prematuration period (PMP), COCs were cultured in T1-non-defined medium (NDM), or T2-defined medium (DM), both for 24 h. In step 2, called the resumption period (RP), COCs were cultured in: NDM (T1); DM + NDM (T3); or DM+DM (T4) for 24 h in each medium. The NDM was composed of TCM-199 supplemented with FCS and FSH. The DM was composed of alpha-MEM supplemented with PVA, insulin, IGF-1, androstenedione, nonessential amino acids, transferrin, and sodium selenium. Oocytes from T2 had a lower (P < 0.05) rate of nuclear maturation (19.8%) than T1 oocytes (83.2%). Also, T2 COCs appeared to be in the process of cytoplasmic maturation, according to the distribution of organelles assessed by transmission electron microscopy (MET). These COCs had characteristics previously described as mature: erect microvilli on the plasmembrane, presence of cortical/evenly distributed mitochondria throughout the ooplasm, and presence of 50% aligned/cluster cortical granules. Immature characteristics such as small PvS, compact cumulus cells, and presence of 50% cortical granule clusters were also observed. The T1 COCs had only characteristics of maturation (P < 0.05). In step 2 (RP), meiosis arrest induced by DM was resumed after an additio... Mostrar Tudo |
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Palavras-Chave: |
Chemically defined medium; Cytoplasmic maturation; Nuclear maturation. |
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Thesaurus Nal: |
Oocytes. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02902naa a2200253 a 4500
001 1909108
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2010.11.021$2DOI
100 1 $aSILVA, I. O. e
245 $aInduction of reversible meiosis arrest of bovine oocytes using a two-step procedure under defined and nondefined conditions.$h[electronic resource]
260 $c2011
520 $aThe objective was to study the effect of a defined culture system, on nuclear and cytoplasmic maturation of bovine oocytes, using the two-step procedure of IVM to detect possible inhibition and subsequent resumption of meiosis arrest. In the first step, called the prematuration period (PMP), COCs were cultured in T1-non-defined medium (NDM), or T2-defined medium (DM), both for 24 h. In step 2, called the resumption period (RP), COCs were cultured in: NDM (T1); DM + NDM (T3); or DM+DM (T4) for 24 h in each medium. The NDM was composed of TCM-199 supplemented with FCS and FSH. The DM was composed of alpha-MEM supplemented with PVA, insulin, IGF-1, androstenedione, nonessential amino acids, transferrin, and sodium selenium. Oocytes from T2 had a lower (P < 0.05) rate of nuclear maturation (19.8%) than T1 oocytes (83.2%). Also, T2 COCs appeared to be in the process of cytoplasmic maturation, according to the distribution of organelles assessed by transmission electron microscopy (MET). These COCs had characteristics previously described as mature: erect microvilli on the plasmembrane, presence of cortical/evenly distributed mitochondria throughout the ooplasm, and presence of 50% aligned/cluster cortical granules. Immature characteristics such as small PvS, compact cumulus cells, and presence of 50% cortical granule clusters were also observed. The T1 COCs had only characteristics of maturation (P < 0.05). In step 2 (RP), meiosis arrest induced by DM was resumed after an additional 24 h of culture in NDM (T3) with 79.2% mature COCs, whereas in T4, meiosis arrest was maintained, resulting in almost 70% immature COCs (P < 0.05). At the end of RP, T3 COCs had the mature characteristics of mitochondria spread throughout the cytoplasm (P < 0.05), cumulus expansion, and alignment of cortical granules, whereas the T4 group had both immature and mature characteristics. We inferred that DM can be used in lieu of meiosis inhibitors and furthermore, it can provide extra time to study nuclear and cytoplasmic maturation synchrony of IVM.
650 $aOocytes
653 $aChemically defined medium
653 $aCytoplasmic maturation
653 $aNuclear maturation
700 1 $aVASCONCELOS, R. B.
700 1 $aCAETANO, J. V. O.
700 1 $aGULART, L. V. M.
700 1 $aCAMARGO, L. S. de A.
700 1 $aBÁO, S. N.
700 1 $aSILVA, A. A. M. R. e
773 $tTheriogenology$gv. 75, n. 6, p. 1115-1124, 2011.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 5 | |
| 2. |  | ROCHA, J. D. M.; OSÓRIO, F. M.; AMARAL, K. D. S. Análise do comércio do caranguejo-uçá, Ucides cordatus (Linnaeus, 1763), no município de Macapá-AP. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA UEAP, 2., 2009, Macapá. Livro de resumos expandidos. Macapá: UEAP, 2009. p. 131-139.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Amapá. |
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| 3. | | TAVARES-DIAS, M.; MONTEIRO, A. M. de C.; AFFONSO, E. G.; AMARAL, K. D. S. Weight-length relationship, condition factor and blood parameters of farmed Cichla temensis Humboldt, 1821 (Cichlidae) in central Amazon. Neotropical Ichthyology, Porto Alegre, v. 9, n. 1, p. 113-119, Mar. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amapá. |
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| 4. | | AMARAL, K. D. S.; VIEIRA, I. M.; OSÓRIO, F. M.; ROCHA, J. D. M.; LIMA, J. de F. Bioecology of the crab Ucides cordatus (Crustacea, Decapoda) in mangroves influenced by the Amazon River, Brazil. Acta Amazonica, Manaus, v. 44, n. 2, p. 213-222, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amapá. |
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| 5. | | LIMA, J. de F.; SILVA, L. M. A. da; SILVA, T. C. da; GARCIA, J. da S.; PEREIRA, I. da S.; AMARAL, K. D. S. Reproductive aspects of Macrobrachium amazonicum (Decapoda: Palaemonidae) in the State of Amapá, Amazon River mouth. Acta Amazonica, Manaus, v. 44, n. 2, p. 245-254, June 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amapá. |
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| Registros recuperados : 5 | |
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