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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pesca e Aquicultura. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Ocidental; Embrapa Pesca e Aquicultura. |
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Data corrente: |
26/01/2023 |
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Data da última atualização: |
10/07/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NUNES, B. R. C.; LOPES, Y. V. de A.; SOUZA, R. H. B. de; PAZDIORA, R. D.; USHIZIMA, T. T.; MEDEIROS, S. P.; CORRÊA, L. T.; CARDOSO, I. I.; ANDRADE, M. V. V.; MACIEL-HONDA, P. O.; CHAGAS, E. C.; SEBASTIÃO, F. de A. |
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Afiliação: |
BRUNA RAFAELA CAETANO NUNES, Universidade Federal de Rondônia; YURI VINICIUS DE ANDRADE LOPES, Universidade Federal do Maranhão; RICARDO HENRIQUE B. DE SOUZA, Universidade Federal de Rondônia; RAUL DIRCEU PAZDIORA, Universidade Federal de Rondônia; THIAGO TETSUO USHIZIMA, Adisseo Brasil Nutrição Animal Ltda; SIMONE PAIVA MEDEIROS, Universidade Federal de Rondônia; LORRAYNE TAVARES CORRÊA, Universidade Federal de Rondônia; IGOR IZIDORO CARDOSO, Universidade Federal de Rondônia; MATHEUS VITOR VIEIRA ANDRADE, Universidade Federal de Rondônia; PATRICIA OLIVEIRA MACIEL HONDA, CNPASA; EDSANDRA CAMPOS CHAGAS, CPAA; FERNANDA DE ALEXANDRE SEBASTIÃO, Universidade Federal do Amazonas. |
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Título: |
Treatments for the control of Neoechinorhynchus buttnerae (Acanthocephala) in tambaqui Colossoma macropomum. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Aquaculture International, v. 31, p. 1821-1835, Jan. 2023. |
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ISSN: |
1573-143X |
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DOI: |
10.1007/s10499-023-01057-z |
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Idioma: |
Inglês |
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Conteúdo: |
Intensive fish farming with inadequate management can imbalance the fish-pathogen-environment relationship, culminating in diseases outbreaks. In the case of tambaqui Colossoma macropomum farming, it may be affected by acanthocephalosis, a parasitic infection without a specific and safe treatment yet. The objective of this study was to evaluate the effect of the active ingredients in trichlorfon, albendazole, piperazine, and ivermectin, as well as the non-traditional chemists calcium hydroxide and diatomaceous earth incorporated to the feed, to control Neoechinorhynchus buttnerae (Acanthocephala) in tambaqui. A 63-day trial was conducted in a completely randomized experimental design, with 7 treatments, 3 replicates each, and 54 fish per treatment. Fish were sampled for weight gain and parasite count on days 0, 15, 35, and 63. In general, the efficacy of anthelmintics was low in the treatment of N. buttnerae in tambaqui. Nevertheless, among the tested products, calcium hydroxide and diatomaceous earth showed promising results (48.2% and 36.4% efficacy, respectively), followed by ivermectin and piperazine (15.9% and 12.6%, respectively). Future studies are warranted to test longer drug administration periods, novel products, and diverse experimental conditions to treat N. buttnerae in tambaqui, to provide information to develop strategies for disease management. |
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Palavras-Chave: |
Helminth; Neoechinorhynchus buttnerae; Treatment. |
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Thesagro: |
Colossoma Macropomum; Peixe; Tambaqui. |
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Thesaurus Nal: |
Calcium hydroxide; Diatomaceous earth; Fish. |
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Categoria do assunto: |
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L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02506naa a2200385 a 4500
001 2151275
005 2023-07-10
008 2023 bl uuuu u00u1 u #d
022 $a1573-143X
024 7 $a10.1007/s10499-023-01057-z$2DOI
100 1 $aNUNES, B. R. C.
245 $aTreatments for the control of Neoechinorhynchus buttnerae (Acanthocephala) in tambaqui Colossoma macropomum.$h[electronic resource]
260 $c2023
520 $aIntensive fish farming with inadequate management can imbalance the fish-pathogen-environment relationship, culminating in diseases outbreaks. In the case of tambaqui Colossoma macropomum farming, it may be affected by acanthocephalosis, a parasitic infection without a specific and safe treatment yet. The objective of this study was to evaluate the effect of the active ingredients in trichlorfon, albendazole, piperazine, and ivermectin, as well as the non-traditional chemists calcium hydroxide and diatomaceous earth incorporated to the feed, to control Neoechinorhynchus buttnerae (Acanthocephala) in tambaqui. A 63-day trial was conducted in a completely randomized experimental design, with 7 treatments, 3 replicates each, and 54 fish per treatment. Fish were sampled for weight gain and parasite count on days 0, 15, 35, and 63. In general, the efficacy of anthelmintics was low in the treatment of N. buttnerae in tambaqui. Nevertheless, among the tested products, calcium hydroxide and diatomaceous earth showed promising results (48.2% and 36.4% efficacy, respectively), followed by ivermectin and piperazine (15.9% and 12.6%, respectively). Future studies are warranted to test longer drug administration periods, novel products, and diverse experimental conditions to treat N. buttnerae in tambaqui, to provide information to develop strategies for disease management.
650 $aCalcium hydroxide
650 $aDiatomaceous earth
650 $aFish
650 $aColossoma Macropomum
650 $aPeixe
650 $aTambaqui
653 $aHelminth
653 $aNeoechinorhynchus buttnerae
653 $aTreatment
700 1 $aLOPES, Y. V. de A.
700 1 $aSOUZA, R. H. B. de
700 1 $aPAZDIORA, R. D.
700 1 $aUSHIZIMA, T. T.
700 1 $aMEDEIROS, S. P.
700 1 $aCORRÊA, L. T.
700 1 $aCARDOSO, I. I.
700 1 $aANDRADE, M. V. V.
700 1 $aMACIEL-HONDA, P. O.
700 1 $aCHAGAS, E. C.
700 1 $aSEBASTIÃO, F. de A.
773 $tAquaculture International$gv. 31, p. 1821-1835, Jan. 2023.
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Registro original: |
Embrapa Pesca e Aquicultura (CNPASA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
17/01/2017 |
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Data da última atualização: |
20/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SANTOS, L. F. dos; SILVA, R. J. S.; AMARAL, D. O. J. do; PAULA, M. F. B. de; FALCÃO, L. L.; LEGAVRE, T.; ALVES, R. M.; MARCELLINO, L. H.; MICHELI, F. |
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Afiliação: |
Lucas Ferraz dos Santos, Universidade Estadual de Santa Cruz; Raner José Santana Silva, Universidade Estadual de Santa Cruz; Daniel Oliveira Jordão do Amaral, Universidade Estadual de Santa Cruz; Márcia Fabiana Barbosa de Paula, Universidade Estadual de Santa Cruz; LOENI LUDKE FALCAO, Cenargen; Thierry Legavre, CIRAD; RAFAEL MOYSES ALVES, CPATU; LUCILIA HELENA MARCELLINO, Cenargen; Fabienne Micheli, Universidade Estadual de Santa Cruz / CIRAD. |
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Título: |
Selection of reference genes for expression study in pulp and seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Plos One, v. 11, n. 8, e0160646, 2016. |
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DOI: |
http://dx.doi.org/10.1371/journal.pone.0160646 |
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Idioma: |
Inglês |
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Conteúdo: |
Cupuassu (Theobroma grandiflorum [Willd. ex Spreng.] Schum) is a species of high economic importance in Brazil with great potential at international level due to the multiple uses of both its seeds and pulp in the industry of sweets and cosmetics. For this reason, the cupuassu breeding program focused on the selection of genotypes with high pulp and seed quality?selection associated with the understanding of the mechanisms involved in fruit formation. Gene expression is one of the most used approaches related to such understanding. In this sense, quantitative real-time PCR (qPCR) is a powerful tool, since it rapidly and reliably quantifies gene expression levels across different experimental conditions. The analysis by qPCR and the correct interpretation of data depend on signal normalization using reference genes, i.e. genes presenting a uniform pattern of expression in the analyzed samples. Here, we selected and analyzed the expression of five genes from cupuassu (ACP, ACT, GAPDH, MDH, TUB) to be used as candidates for reference genes on pulp and seed of young, maturing and mature cupuassu fruits. The evaluation of the gene expression stability was obtained using the NormFinder, geNorm and BestKeeper programs. In general, our results indicated that the GAPDH and MDH genes constituted the best combination as reference genes to analyze the expression of cupuassu samples. To our knowledge, this is the first report of reference gene definition in cupuassu, and these results will support subsequent analysis related to gene expression studies in cupuassu plants subjected to different biotic or abiotic conditions as well as serve as a tool for diversity analysis based on pulp and seed quality. MenosCupuassu (Theobroma grandiflorum [Willd. ex Spreng.] Schum) is a species of high economic importance in Brazil with great potential at international level due to the multiple uses of both its seeds and pulp in the industry of sweets and cosmetics. For this reason, the cupuassu breeding program focused on the selection of genotypes with high pulp and seed quality?selection associated with the understanding of the mechanisms involved in fruit formation. Gene expression is one of the most used approaches related to such understanding. In this sense, quantitative real-time PCR (qPCR) is a powerful tool, since it rapidly and reliably quantifies gene expression levels across different experimental conditions. The analysis by qPCR and the correct interpretation of data depend on signal normalization using reference genes, i.e. genes presenting a uniform pattern of expression in the analyzed samples. Here, we selected and analyzed the expression of five genes from cupuassu (ACP, ACT, GAPDH, MDH, TUB) to be used as candidates for reference genes on pulp and seed of young, maturing and mature cupuassu fruits. The evaluation of the gene expression stability was obtained using the NormFinder, geNorm and BestKeeper programs. In general, our results indicated that the GAPDH and MDH genes constituted the best combination as reference genes to analyze the expression of cupuassu samples. To our knowledge, this is the first report of reference gene definition in cupuassu, and these results wi... Mostrar Tudo |
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Palavras-Chave: |
Genotipos. |
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Thesagro: |
Cupuaçu. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/153764/1/document-4.pdf
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Marc: |
LEADER 02498naa a2200253 a 4500
001 2060964
005 2022-05-20
008 2016 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1371/journal.pone.0160646$2DOI
100 1 $aSANTOS, L. F. dos
245 $aSelection of reference genes for expression study in pulp and seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum.$h[electronic resource]
260 $c2016
520 $aCupuassu (Theobroma grandiflorum [Willd. ex Spreng.] Schum) is a species of high economic importance in Brazil with great potential at international level due to the multiple uses of both its seeds and pulp in the industry of sweets and cosmetics. For this reason, the cupuassu breeding program focused on the selection of genotypes with high pulp and seed quality?selection associated with the understanding of the mechanisms involved in fruit formation. Gene expression is one of the most used approaches related to such understanding. In this sense, quantitative real-time PCR (qPCR) is a powerful tool, since it rapidly and reliably quantifies gene expression levels across different experimental conditions. The analysis by qPCR and the correct interpretation of data depend on signal normalization using reference genes, i.e. genes presenting a uniform pattern of expression in the analyzed samples. Here, we selected and analyzed the expression of five genes from cupuassu (ACP, ACT, GAPDH, MDH, TUB) to be used as candidates for reference genes on pulp and seed of young, maturing and mature cupuassu fruits. The evaluation of the gene expression stability was obtained using the NormFinder, geNorm and BestKeeper programs. In general, our results indicated that the GAPDH and MDH genes constituted the best combination as reference genes to analyze the expression of cupuassu samples. To our knowledge, this is the first report of reference gene definition in cupuassu, and these results will support subsequent analysis related to gene expression studies in cupuassu plants subjected to different biotic or abiotic conditions as well as serve as a tool for diversity analysis based on pulp and seed quality.
650 $aCupuaçu
653 $aGenotipos
700 1 $aSILVA, R. J. S.
700 1 $aAMARAL, D. O. J. do
700 1 $aPAULA, M. F. B. de
700 1 $aFALCÃO, L. L.
700 1 $aLEGAVRE, T.
700 1 $aALVES, R. M.
700 1 $aMARCELLINO, L. H.
700 1 $aMICHELI, F.
773 $tPlos One$gv. 11, n. 8, e0160646, 2016.
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