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Registro Completo |
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Biblioteca(s): |
Embrapa Clima Temperado; Embrapa Trigo. |
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Data corrente: |
08/03/2024 |
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Data da última atualização: |
08/03/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CASASSOLA, A.; EREFUL, N. C.; ZANELLA, C. M.; PANDEY, P.; BRAMMER, S. P.; CHAVES, M. S.; MARTINELLI, J. A.; BOYD, L. A. |
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Afiliação: |
ALICE CASASSOLA; NELZO C. EREFUL; CAMILA M. ZANELLA; POOJA PANDEY; SANDRA PATUSSI BRAMMER, CNPT; MARCIA SOARES CHAVES, CPACT; JOSÉ A. MARTINELLI, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL; LESLEY A. BOYD. |
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Título: |
Transcriptional profiling identifies the early responses to Puccinia triticina infection in the adult plant leaf rust resistant wheat variety Toropi. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Plant Pathology, 2024. |
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DOI: |
10.1111/ppa.13865 |
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Idioma: |
Inglês |
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Notas: |
Online first. Early view. |
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Conteúdo: |
Leaf rust, caused by Puccinia triticina (Pt), is a major disease of wheat and a significant problem for wheat production in Brazil. The Brazilian variety Toropi, released in 1965, has maintained high levels of field, adult plant resistance (APR) to leaf rust across global locations, while microscopic studies have indicated prehaustorial resistance mechanisms. Analyses of gene expression in flag leaves of Toropi, during the early stages of Pt infection, were undertaken to explore the mechanisms behind the APR in Toropi. Differential expression of wheat genes was undertaken, comparing Pt- to mock- inoculated and Pt- to Pt- inoculated time points. Analysis of gene expression indicated a strong response to Pt, which was fully active by 6 h after inoculation (hai). More genes were downregulated than upregulated, particularly at 6 and 12 hai. Gene Ontology enrichment analysis indicated a shutting down of RNA and protein synthe-sis and an early effect on photosynthesis, with disruption of the electron transfer chain. Analyses of upregulated genes identified genes involved in ATP-binding and protein kinase activity at 6 hai, supporting a rapid metabolic response to Pt infection. A general upregulation of genes involved in transport and metabolism indicated the need to relocate protein and organic- based resources. Alignment of differentially ex-pressed genes with the genomic regions defining four leaf rust APR quantitative trait loci (QTLs) in Toropi identified candidate resistance genes, including a sugar trans-porter, a receptor kinase and a seven-transmembrane MLO family protein. In addition, 60 Pt genes were identified, 11 being annotated as potential effector proteins. MenosLeaf rust, caused by Puccinia triticina (Pt), is a major disease of wheat and a significant problem for wheat production in Brazil. The Brazilian variety Toropi, released in 1965, has maintained high levels of field, adult plant resistance (APR) to leaf rust across global locations, while microscopic studies have indicated prehaustorial resistance mechanisms. Analyses of gene expression in flag leaves of Toropi, during the early stages of Pt infection, were undertaken to explore the mechanisms behind the APR in Toropi. Differential expression of wheat genes was undertaken, comparing Pt- to mock- inoculated and Pt- to Pt- inoculated time points. Analysis of gene expression indicated a strong response to Pt, which was fully active by 6 h after inoculation (hai). More genes were downregulated than upregulated, particularly at 6 and 12 hai. Gene Ontology enrichment analysis indicated a shutting down of RNA and protein synthe-sis and an early effect on photosynthesis, with disruption of the electron transfer chain. Analyses of upregulated genes identified genes involved in ATP-binding and protein kinase activity at 6 hai, supporting a rapid metabolic response to Pt infection. A general upregulation of genes involved in transport and metabolism indicated the need to relocate protein and organic- based resources. Alignment of differentially ex-pressed genes with the genomic regions defining four leaf rust APR quantitative trait loci (QTLs... Mostrar Tudo |
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Palavras-Chave: |
Puccinia triticina. |
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Thesagro: |
Ferrugem; Resistência; Trigo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02569naa a2200277 a 4500
001 2162709
005 2024-03-08
008 2024 bl uuuu u00u1 u #d
024 7 $a10.1111/ppa.13865$2DOI
100 1 $aCASASSOLA, A.
245 $aTranscriptional profiling identifies the early responses to Puccinia triticina infection in the adult plant leaf rust resistant wheat variety Toropi.$h[electronic resource]
260 $c2024
500 $aOnline first. Early view.
520 $aLeaf rust, caused by Puccinia triticina (Pt), is a major disease of wheat and a significant problem for wheat production in Brazil. The Brazilian variety Toropi, released in 1965, has maintained high levels of field, adult plant resistance (APR) to leaf rust across global locations, while microscopic studies have indicated prehaustorial resistance mechanisms. Analyses of gene expression in flag leaves of Toropi, during the early stages of Pt infection, were undertaken to explore the mechanisms behind the APR in Toropi. Differential expression of wheat genes was undertaken, comparing Pt- to mock- inoculated and Pt- to Pt- inoculated time points. Analysis of gene expression indicated a strong response to Pt, which was fully active by 6 h after inoculation (hai). More genes were downregulated than upregulated, particularly at 6 and 12 hai. Gene Ontology enrichment analysis indicated a shutting down of RNA and protein synthe-sis and an early effect on photosynthesis, with disruption of the electron transfer chain. Analyses of upregulated genes identified genes involved in ATP-binding and protein kinase activity at 6 hai, supporting a rapid metabolic response to Pt infection. A general upregulation of genes involved in transport and metabolism indicated the need to relocate protein and organic- based resources. Alignment of differentially ex-pressed genes with the genomic regions defining four leaf rust APR quantitative trait loci (QTLs) in Toropi identified candidate resistance genes, including a sugar trans-porter, a receptor kinase and a seven-transmembrane MLO family protein. In addition, 60 Pt genes were identified, 11 being annotated as potential effector proteins.
650 $aFerrugem
650 $aResistência
650 $aTrigo
653 $aPuccinia triticina
700 1 $aEREFUL, N. C.
700 1 $aZANELLA, C. M.
700 1 $aPANDEY, P.
700 1 $aBRAMMER, S. P.
700 1 $aCHAVES, M. S.
700 1 $aMARTINELLI, J. A.
700 1 $aBOYD, L. A.
773 $tPlant Pathology, 2024.
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Registro original: |
Embrapa Trigo (CNPT) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
28/03/2009 |
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Data da última atualização: |
10/07/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
FREITAS-ASTÚA, F.; SILVA-PINHATI, A. C. O.; BERGER, I. J.; LUCHETA, A. R.; BASÍLIO-PALMIERI, A. C.; AMARAL, A. M. do; BARBOSA, A. V. G.; MACHADO, M. A. |
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Afiliação: |
Juliana Freitas-Ástua, CNPMF; Ana Carla O. Silva-Pinhati, APTA; Irving J. Berger, APTA; Adriano R. Lucheta, APTA; Ana Carolina Basílio-Palmieri, APTA; Alexandre M. do Amaral, CENARGEN; Andréa V.G. Barbosa, APTA; Marcos A. Machado, APTA. |
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Título: |
Putative novel genes involved in the biosynthesis of sweet orange flavonoids. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 16., 2008, San Diego. Final abstracts guide. San Diego: Illumina, 2008. |
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Idioma: |
Inglês |
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Notas: |
P19 |
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Conteúdo: |
Flavonoids constitute a major group of secondary metabolites and play important roles in the development, reproduction, defense, and pigmentation of plants. Flavones represent one of the most abundant and important classes of flavonoids, occurring in almost all plant tissues. The formation of flavones is catalyzed by either flavone synthase I or flavone synthase II (FNSII). Both enzymes enable the control of a biosynthetic step at an important junction of this pathway leading not only to flavones but also to various flavonoid classes, as isoflavones, flavonols, flavanols, and anthocyanins. Flavonols are important co-pigments that may affect the shade of the color in flowers and fruits. One of the main enzymes in the flavonol biosynthesis pathway is flavanone-3-hydroxylase (F3H), which follows chalcone isomerase in the pathway and hydroxylates flavanones at the 3´ position to form dihydroflavonols. Analyses of the Citrus EST (CitEST) database revealed the presence of putative novel fnsII and f3h genes involved in the synthesis of flavones and flavonols, respectively, in Citrus sinensis. Even though flavones have been found in several species and varieties within the Citrus genus, this is the first study to report the presence of two copies of the fnsII gene in sweet orange. Moreover, in addition to the available Citf3h gene sequence, we identified another f3h transcript in C. sinensis. Here we report the in silico characterization of such genes expression patterns in different citrus tissues and fruit developmental stages, and provide phylogenetic analyses of their deduced amino acid sequences MenosFlavonoids constitute a major group of secondary metabolites and play important roles in the development, reproduction, defense, and pigmentation of plants. Flavones represent one of the most abundant and important classes of flavonoids, occurring in almost all plant tissues. The formation of flavones is catalyzed by either flavone synthase I or flavone synthase II (FNSII). Both enzymes enable the control of a biosynthetic step at an important junction of this pathway leading not only to flavones but also to various flavonoid classes, as isoflavones, flavonols, flavanols, and anthocyanins. Flavonols are important co-pigments that may affect the shade of the color in flowers and fruits. One of the main enzymes in the flavonol biosynthesis pathway is flavanone-3-hydroxylase (F3H), which follows chalcone isomerase in the pathway and hydroxylates flavanones at the 3´ position to form dihydroflavonols. Analyses of the Citrus EST (CitEST) database revealed the presence of putative novel fnsII and f3h genes involved in the synthesis of flavones and flavonols, respectively, in Citrus sinensis. Even though flavones have been found in several species and varieties within the Citrus genus, this is the first study to report the presence of two copies of the fnsII gene in sweet orange. Moreover, in addition to the available Citf3h gene sequence, we identified another f3h transcript in C. sinensis. Here we report the in silico characterization of such genes expression patterns in differen... Mostrar Tudo |
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Thesagro: |
Composto Fenólico; Flavonóide; Fruta Cítrica; Gene. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02455nam a2200253 a 4500
001 1655630
005 2023-07-10
008 2008 bl uuuu u00u1 u #d
100 1 $aFREITAS-ASTÚA, F.
245 $aPutative novel genes involved in the biosynthesis of sweet orange flavonoids.$h[electronic resource]
260 $aIn: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 16., 2008, San Diego. Final abstracts guide. San Diego: Illumina$c2008
500 $aP19
520 $aFlavonoids constitute a major group of secondary metabolites and play important roles in the development, reproduction, defense, and pigmentation of plants. Flavones represent one of the most abundant and important classes of flavonoids, occurring in almost all plant tissues. The formation of flavones is catalyzed by either flavone synthase I or flavone synthase II (FNSII). Both enzymes enable the control of a biosynthetic step at an important junction of this pathway leading not only to flavones but also to various flavonoid classes, as isoflavones, flavonols, flavanols, and anthocyanins. Flavonols are important co-pigments that may affect the shade of the color in flowers and fruits. One of the main enzymes in the flavonol biosynthesis pathway is flavanone-3-hydroxylase (F3H), which follows chalcone isomerase in the pathway and hydroxylates flavanones at the 3´ position to form dihydroflavonols. Analyses of the Citrus EST (CitEST) database revealed the presence of putative novel fnsII and f3h genes involved in the synthesis of flavones and flavonols, respectively, in Citrus sinensis. Even though flavones have been found in several species and varieties within the Citrus genus, this is the first study to report the presence of two copies of the fnsII gene in sweet orange. Moreover, in addition to the available Citf3h gene sequence, we identified another f3h transcript in C. sinensis. Here we report the in silico characterization of such genes expression patterns in different citrus tissues and fruit developmental stages, and provide phylogenetic analyses of their deduced amino acid sequences
650 $aComposto Fenólico
650 $aFlavonóide
650 $aFruta Cítrica
650 $aGene
700 1 $aSILVA-PINHATI, A. C. O.
700 1 $aBERGER, I. J.
700 1 $aLUCHETA, A. R.
700 1 $aBASÍLIO-PALMIERI, A. C.
700 1 $aAMARAL, A. M. do
700 1 $aBARBOSA, A. V. G.
700 1 $aMACHADO, M. A.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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