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| 52. |  | RITTERBUSCH, G. A.; SÁ ROCHA, C.; SIMON, N. L.; MORES, N.; AMARAL, A. L. do; ZANELLA, J. R. C. Baixa presença de circovírus suíno tipo 1 e tipo 2 (PCV1 e PCV2) em órgãos reprodutivos e fluido folicular ovariano de fêmeas descartadas In: CONGRESSO BRASILEIRO DE VETERINÁRIOS ESPECIALISTAS EM SUÍNOS, 14., 2009, Uberlândia. Anais... Uberlândia: Abraves, 2009. p. 315-316. 1 CD-ROM. Subprojeto: 03.07.51.900-03| Biblioteca(s): Embrapa Suínos e Aves. |
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| 56. | | ROCHA, C. S.; RITTERBUSCH, G. A.; SIMON, N. L.; ZANELLA, J. R. C.; MORES, N.; AMARAL, A. L. do. Pesquisa de parvovírus suíno em aparelho reprodutivo de fêmeas abatidas em frigoríficos do meio-oeste do estado de Santa Catarina In: CONGRESSO BRASILEIRO DE VETERINÁRIOS ESPECIALISTAS EM SUÍNOS, 14., 2009, Uberlândia. Anais... Uberlândia: Abraves, 2009. p. 299-300. 1 CD-ROM. Subprojeto: 03.07.51.900-03| Biblioteca(s): Embrapa Suínos e Aves. |
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| 57. | | DALLA COSTA, O. A.; AMARAL, A. L. do; LUDKE, J. V.; COLDEBELLA, A.; FIGUEIREDO, E. A. P. Desempenho, características de carcaça, qualidade da carne e condição sanitária de suínos criados nas fases de crescimento e terminação nos sistemas confinado convencional e de cama sobreposta. Ciência Rural, v.38, n.8, p.2307-2313, 2008. Projeto/Plano de Ação: 01.06.60.107-06.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 59. | | OLIVEIRA, A. C. A. de; OLIVEIRA, L. A. R. de; AMARAL, A. L. do; LEDO, A. da S. Efeito da solução de vitrificação PVS3 na regeneração de meristemas apicais de cana-de-açúcar. Revista RG News, v. 4, n. 3, p. 421, 2018. Edição especial dos Anais do 5º Congresso Brasileiro de Recursos Genéticos, Fortaleza, nov. 2018.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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Registro Completo
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
22/12/2020 |
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Data da última atualização: |
22/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 4 |
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Autoria: |
LEDO, A. da S.; AMARAL, A. L. do; JENDEREK, M. M.; HARRISON, M.; MANTER, D. K. |
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Afiliação: |
ANA DA SILVA LEDO, CPATC; ADRIANE LEITE DO AMARAL, CPATC; MARIA M. JENDEREK; MELAINE HARRISON; DANIEL K. MANTER. |
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Título: |
Sterilization procedures and Plant Preservative Mixture on in vitro establishment of Miscanthus sinensis Andersson. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Plant Cell Culture e Micropropagation, v. 15, n. 2, p. 27-32, 2019. |
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ISSN: |
1808-9909 |
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DOI: |
10.46526/pccm.2019.v15i2.139 |
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Idioma: |
Inglês |
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Conteúdo: |
Information about the establishment phase of Miscanthus sinensis Andersson as sterile procedure to avoid the contamination were few reported. The aim of this study was to evaluate different immersion sterilization procedures with and without the biocide, Plant Preservative Mixture (PPM?) on in vitro contamination of two Miscanthus genotypes. The plant material were canes from adult plants of two accessions PI 668371 and PI 668375 cultivated in the germplasm bank of USDA-ARS, Griffin, Georgia. Apical meristems were submitted to immersion sterilization procedures (70% isopropyl alcohol and 1.5, 5.0 or 8.25% NaOCl for different time periods, followed by rinsing three times with autoclaved distilled water. After that, the explants were inoculated on MS basal medium in the presence or absence of 1 mL L-1 PPM?. The experimental design was fully randomized in a 2 x 5 x 2 factorial scheme (genotypes x sterilization process x PPM?) with five replicates per treatment and five apical meristems per experimental unit. Aseptic treatments showed no differences for percentage of bacterial and fungal contaminations and percentage of explant survival. In the presence of PPM ? there was less bacterial contamination in the accessions (28%) than in the absence (100%). For the percentage of fungal contamination, PPM ? had no significant effect on PI 668371 accession. However, on PI 668375 accession there was a lower percentage of fungal contamination (16%). The biocide PPM ? may be an efficient agent to prevent bacterial contamination on in vitro cultures of Miscanthus spp and fungal contamination in PI 668375 accession. MenosInformation about the establishment phase of Miscanthus sinensis Andersson as sterile procedure to avoid the contamination were few reported. The aim of this study was to evaluate different immersion sterilization procedures with and without the biocide, Plant Preservative Mixture (PPM?) on in vitro contamination of two Miscanthus genotypes. The plant material were canes from adult plants of two accessions PI 668371 and PI 668375 cultivated in the germplasm bank of USDA-ARS, Griffin, Georgia. Apical meristems were submitted to immersion sterilization procedures (70% isopropyl alcohol and 1.5, 5.0 or 8.25% NaOCl for different time periods, followed by rinsing three times with autoclaved distilled water. After that, the explants were inoculated on MS basal medium in the presence or absence of 1 mL L-1 PPM?. The experimental design was fully randomized in a 2 x 5 x 2 factorial scheme (genotypes x sterilization process x PPM?) with five replicates per treatment and five apical meristems per experimental unit. Aseptic treatments showed no differences for percentage of bacterial and fungal contaminations and percentage of explant survival. In the presence of PPM ? there was less bacterial contamination in the accessions (28%) than in the absence (100%). For the percentage of fungal contamination, PPM ? had no significant effect on PI 668371 accession. However, on PI 668375 accession there was a lower percentage of fungal contamination (16%). The biocide PPM ? may be an efficient a... Mostrar Tudo |
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Thesagro: |
Contaminação Fúngica; Cultura In Vitro; Micropropagação. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219589/1/16.-Ana-Ledo-Sterilization-procedures-and-plant.pdf
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Marc: |
LEADER 02363naa a2200229 a 4500
001 2128617
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008 2019 bl uuuu u00u1 u #d
022 $a1808-9909
024 7 $a10.46526/pccm.2019.v15i2.139$2DOI
100 1 $aLEDO, A. da S.
245 $aSterilization procedures and Plant Preservative Mixture on in vitro establishment of Miscanthus sinensis Andersson.$h[electronic resource]
260 $c2019
520 $aInformation about the establishment phase of Miscanthus sinensis Andersson as sterile procedure to avoid the contamination were few reported. The aim of this study was to evaluate different immersion sterilization procedures with and without the biocide, Plant Preservative Mixture (PPM?) on in vitro contamination of two Miscanthus genotypes. The plant material were canes from adult plants of two accessions PI 668371 and PI 668375 cultivated in the germplasm bank of USDA-ARS, Griffin, Georgia. Apical meristems were submitted to immersion sterilization procedures (70% isopropyl alcohol and 1.5, 5.0 or 8.25% NaOCl for different time periods, followed by rinsing three times with autoclaved distilled water. After that, the explants were inoculated on MS basal medium in the presence or absence of 1 mL L-1 PPM?. The experimental design was fully randomized in a 2 x 5 x 2 factorial scheme (genotypes x sterilization process x PPM?) with five replicates per treatment and five apical meristems per experimental unit. Aseptic treatments showed no differences for percentage of bacterial and fungal contaminations and percentage of explant survival. In the presence of PPM ? there was less bacterial contamination in the accessions (28%) than in the absence (100%). For the percentage of fungal contamination, PPM ? had no significant effect on PI 668371 accession. However, on PI 668375 accession there was a lower percentage of fungal contamination (16%). The biocide PPM ? may be an efficient agent to prevent bacterial contamination on in vitro cultures of Miscanthus spp and fungal contamination in PI 668375 accession.
650 $aContaminação Fúngica
650 $aCultura In Vitro
650 $aMicropropagação
700 1 $aAMARAL, A. L. do
700 1 $aJENDEREK, M. M.
700 1 $aHARRISON, M.
700 1 $aMANTER, D. K.
773 $tPlant Cell Culture e Micropropagation$gv. 15, n. 2, p. 27-32, 2019.
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