Data/hora: 09/06/2024 19:01:24

Biblioteca(s): | Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia.
Data corrente: | 21/01/2019
Data da última atualização: | 12/02/2020
Autoria: | ALVES-FREITAS, D. M. T.; PINHEIRO-LIMA, B.; FARIA, J. C.; LACORTE, C.; RIBEIRO, S. G.; MELO, F. L.
Afiliação: | DIONE M. T. ALVES-FREITAS; BRUNA PINHEIRO-LIMA; JOSIAS CORREA DE FARIA, CNPAF; CRISTIANO CASTRO LACORTE, Cenargen; SIMONE DA GRACA RIBEIRO, Cenargen; FERNANDO L. MELO, UNB.
Título: | Double-stranded RNA high-throughput sequencing reveals a new Cytorhabdovirus in a bean golden mosaic virus-resistant common bean transgenic line.
Ano de publicação: | 2019
Fonte/Imprenta: | Viruses, v. 11, n. 1, Jan. 2019.
ISSN: | 1999-4915
DOI: | 10.3390/v11010090
Idioma: | Inglês
Conteúdo: | Using double-strand RNA (dsRNA) high-throughput sequencing, we identified five RNA viruses in a bean golden mosaic virus (BGMV)-resistant common bean transgenic line with symptoms of viral infection. Four of the identified viruses had already been described as infecting common bean (cowpea mild mottle virus, bean rugose mosaic virus, Phaseolus vulgaris alphaendornavirus 1, and Phaseolus vulgaris alphaendornavirus 2) and one is a putative new plant rhabdovirus (genus Cytorhabdovirus), tentatively named bean-associated cytorhabdovirus (BaCV). The BaCV genome presented all five open reading frames (ORFs) found in most rhabdoviruses: nucleoprotein (N) (ORF1) (451 amino acids, aa), phosphoprotein (P) (ORF2) (445 aa), matrix (M) (ORF4) (287 aa), glycoprotein (G) (ORF5) (520 aa), and an RNA-dependent RNA polymerase (L) (ORF6) (114 aa), as well as a putative movement protein (P3) (ORF3) (189 aa) and the hypothetical small protein P4. The predicted BaCV proteins were compared to homologous proteins from the closest cytorhabdoviruses, and a low level of sequence identity (15?39%) was observed. The phylogenetic analysis shows that BaCV clustered with yerba mate chlorosis-associated virus (YmCaV) and rice stripe mosaic virus (RSMV). Overall, our results provide strong evidence that BaCV is indeed a new virus species in the genus Cytorhabdovirus (family Rhabdoviridae), the first rhabdovirus to be identified infecting common bean.
Palavras-Chave: | Rhabdovirus.
Thesagro: | Feijão; Mosaico Dourado; Organismo Transgênico; Phaseolus Vulgaris; Vírus.
Thesaurus Nal: | Bean golden mosaic virus; Beans; Cytorhabdovirus; DsRNA viruses; Transgenic plants.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/191029/1/CNPAF-2019-viruses.pdf
Registro original: | Embrapa Arroz e Feijão (CNPAF)

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CNPAF - (35429-1) - UPC - AP - DD - 2019 -  - 2019 - DIS
CENARGEN - (37610-1) - UPC - AP - DD -  -  -  - DIS