| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
|
Data corrente: |
19/07/2017 |
|
Data da última atualização: |
19/07/2017 |
|
Autoria: |
KANAI, Y.; YAMASAKI, J.; TAKEUCHI, Y.; KOIKE, H.; FUJIYAMA, M.; MORI, Y. |
|
Título: |
Electrophysiological recording of hypothalamic gnrh pulse generator activity in castrated male goats. |
|
Ano de publicação: |
1995 |
|
Fonte/Imprenta: |
Journal of Reproduction and Development, v. 41, n. 1, p. 57-62, Fev. 1995. |
|
DOI: |
http://doi.org/10.1262/jrd.41.57 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Electrophysiological recording of the hypothalamic GnRH pulse generator activity was attempted in 3 long-term castrated male goats in which electrodes had been implanted in the medial basal hypothalamus (MBH). While multiple unit activity (MUA) signals from the electrode were continuously recorded, each animal received subcutaneous implants containing testosterone for 7 days (Experiment 1). They were similarly treated with estradiol alone, estradiol plus testosterone, and testosterone alone for 7 days each consecutively (Experiment 2). These steroidal treatments enabled to maintain the plasma levels of testosterone and estradiol within physiological ranges at 2-4 ng/ml and 3-5 pg/ml, respectively. Blood samples for the determination of pulsatile LH secretion were collected at 6-min intervals for 4 h on the last day of each treatment period. The short-lived sudden increase in MUA spikes (MUA volley) was detected being always preceding the occurrence of the LH pulse in the peripheral blood, and this synchrony of MUA volley with the LH pulse was constant in all the animals regardless of steroidal treatments. Testosterone treatment resulted in the increase of the MUA volley intervals from 27 min to 43 min (Experiment 1). Treatment with estradiol in Experiment 2 also resulted in prolonged MUA volley intervals similar to those of testosterone treatment and there was no additive or synergistic effect between these two steroids. The present study demonstrated that the electrophysiological manifestation of the hypothalamic GnRH pulse generator could be successfully monitored in male goats as reported in females, and it is suggested that estradiol may exert a negative feedback effect on the GnRH pulse generator activity in a similar manner to that of testosterone but at much lower doses in male goats. MenosElectrophysiological recording of the hypothalamic GnRH pulse generator activity was attempted in 3 long-term castrated male goats in which electrodes had been implanted in the medial basal hypothalamus (MBH). While multiple unit activity (MUA) signals from the electrode were continuously recorded, each animal received subcutaneous implants containing testosterone for 7 days (Experiment 1). They were similarly treated with estradiol alone, estradiol plus testosterone, and testosterone alone for 7 days each consecutively (Experiment 2). These steroidal treatments enabled to maintain the plasma levels of testosterone and estradiol within physiological ranges at 2-4 ng/ml and 3-5 pg/ml, respectively. Blood samples for the determination of pulsatile LH secretion were collected at 6-min intervals for 4 h on the last day of each treatment period. The short-lived sudden increase in MUA spikes (MUA volley) was detected being always preceding the occurrence of the LH pulse in the peripheral blood, and this synchrony of MUA volley with the LH pulse was constant in all the animals regardless of steroidal treatments. Testosterone treatment resulted in the increase of the MUA volley intervals from 27 min to 43 min (Experiment 1). Treatment with estradiol in Experiment 2 also resulted in prolonged MUA volley intervals similar to those of testosterone treatment and there was no additive or synergistic effect between these two steroids. The present study demonstrated that the electrophysiol... Mostrar Tudo |
|
Palavras-Chave: |
GnRH; GnRH pulse generator; Gonadal steroids; LH; MUA volley. |
|
Thesaurus Nal: |
Goats. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02568naa a2200265 a 4500
001 2072873
005 2017-07-19
008 1995 bl uuuu u00u1 u #d
024 7 $ahttp://doi.org/10.1262/jrd.41.57$2DOI
100 1 $aKANAI, Y.
245 $aElectrophysiological recording of hypothalamic gnrh pulse generator activity in castrated male goats.
260 $c1995
520 $aElectrophysiological recording of the hypothalamic GnRH pulse generator activity was attempted in 3 long-term castrated male goats in which electrodes had been implanted in the medial basal hypothalamus (MBH). While multiple unit activity (MUA) signals from the electrode were continuously recorded, each animal received subcutaneous implants containing testosterone for 7 days (Experiment 1). They were similarly treated with estradiol alone, estradiol plus testosterone, and testosterone alone for 7 days each consecutively (Experiment 2). These steroidal treatments enabled to maintain the plasma levels of testosterone and estradiol within physiological ranges at 2-4 ng/ml and 3-5 pg/ml, respectively. Blood samples for the determination of pulsatile LH secretion were collected at 6-min intervals for 4 h on the last day of each treatment period. The short-lived sudden increase in MUA spikes (MUA volley) was detected being always preceding the occurrence of the LH pulse in the peripheral blood, and this synchrony of MUA volley with the LH pulse was constant in all the animals regardless of steroidal treatments. Testosterone treatment resulted in the increase of the MUA volley intervals from 27 min to 43 min (Experiment 1). Treatment with estradiol in Experiment 2 also resulted in prolonged MUA volley intervals similar to those of testosterone treatment and there was no additive or synergistic effect between these two steroids. The present study demonstrated that the electrophysiological manifestation of the hypothalamic GnRH pulse generator could be successfully monitored in male goats as reported in females, and it is suggested that estradiol may exert a negative feedback effect on the GnRH pulse generator activity in a similar manner to that of testosterone but at much lower doses in male goats.
650 $aGoats
653 $aGnRH
653 $aGnRH pulse generator
653 $aGonadal steroids
653 $aLH
653 $aMUA volley
700 1 $aYAMASAKI, J.
700 1 $aTAKEUCHI, Y.
700 1 $aKOIKE, H.
700 1 $aFUJIYAMA, M.
700 1 $aMORI, Y.
773 $tJournal of Reproduction and Development$gv. 41, n. 1, p. 57-62, Fev. 1995.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Algodão. Para informações adicionais entre em contato com cnpa.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Algodão. |
|
Data corrente: |
01/11/2007 |
|
Data da última atualização: |
24/01/2008 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Circulação/Nível: |
-- - -- |
|
Autoria: |
ALMEIDA, R. P. de; FERNANDES, O. A. |
|
Afiliação: |
Raul Porfírio de Almeida, Embrapa Algodão; Odair Aparecido Fernandes, UNESP. |
|
Título: |
MIP-amendoim em áreas de produção integrada no Brasil. |
|
Ano de publicação: |
2007 |
|
Fonte/Imprenta: |
In: SIMPÓSIO DE ENTOMOLOGIA, 1.; REUNIÃO ANUAL DA SOCIEDADE DE ENTOMOLOGIA DA PARAÍBA, 3., 2007, Campina Grande, PB. Anais... Campina Grande, PB: SEPB, 2007. |
|
Páginas: |
p. 46 |
|
Idioma: |
Português |
|
Conteúdo: |
Manejo integrado de pragas; Arachis hypogea; Produção integrada de amendoim |
|
Palavras-Chave: |
Arachis hypogea; Manejo integrado de pragas; Produção integrada de amendoim. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 00701naa a2200181 a 4500
001 1276044
005 2008-01-24
008 2007 bl uuuu u00u1 u #d
100 1 $aALMEIDA, R. P. de
245 $aMIP-amendoim em áreas de produção integrada no Brasil.
260 $c2007
300 $ap. 46
520 $aManejo integrado de pragas; Arachis hypogea; Produção integrada de amendoim
653 $aArachis hypogea
653 $aManejo integrado de pragas
653 $aProdução integrada de amendoim
700 1 $aFERNANDES, O. A.
773 $tIn: SIMPÓSIO DE ENTOMOLOGIA, 1.; REUNIÃO ANUAL DA SOCIEDADE DE ENTOMOLOGIA DA PARAÍBA, 3., 2007, Campina Grande, PB. Anais... Campina Grande, PB: SEPB, 2007.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Algodão (CNPA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
