Data/hora: 06/06/2024 05:40:06

Biblioteca(s): | Embrapa Algodão.
Data corrente: | 22/03/2016
Data da última atualização: | 22/03/2016
Autoria: | ALMEIDA, R. P. de; STOUTHAMER, R.
Afiliação: | RAUL PORFIRIO DE ALMEIDA, CNPA; R. STOUTHAMER, UNIVERSITY OF CALIFORNIA - USA.
Título: | ITS-2 sequences-based identification of Trichogramma species in South America.
Ano de publicação: | 2015
Fonte/Imprenta: | Brazilian Journal of Biology, v. 75, n. 4, p. 974-982, 2015.
Idioma: | Inglês
Conteúdo: | ITS2 (Internal transcribed spacer 2) sequences have been used in systematic studies and proved to be useful in providing a reliable identification of Trichogramma species. DNAr sequences ranged in size from 379 to 632 bp. In eleven T. pretiosum lines Wolbachia-induced parthenogenesis was found for the first time. These thelytokous lines were collected in Peru (9), Colombia (1) and USA (1). A dichotomous key for species identification was built based on the size of the ITS2 PCR product and restriction analysis using three endonucleases (EcoRI, MseI and MaeI). This molecular technique was successfully used to distinguish among seventeen native/introduced Trichogramma species collected in South America.
Palavras-Chave: | Egg parasitoid; Molecular markers.
Thesagro: | DNA; Himenoptero; Marcador molecular; Trichogramma sp.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/141499/1/ITS-2-sequences-based-identification-....pdf
Registro original: | Embrapa Algodão (CNPA)
Exemplares: | CNPA - (28127-1) - UPC - AP - DD -  -  -  - DIS