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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
26/11/2024 |
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Data da última atualização: |
09/01/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
JIMENEZ, E. J. M.; MARTINS, P. M. M.; ASSIS, J. G. R. de; BATISTA, N. N.; VILELA, A. L. de O.; ROSA, S. D. V. F. da; DIAS, D. R.; SCHWAN, R. F. S. |
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Afiliação: |
EMERSON JOSUE MARTINEZ JIMENEZ, UNIVERSIDAD NACIONAL DE AGRICULTURA; PAMELA MYNSEN MACHADO MARTINS, UNIVERSIDADE FEDERAL DE LAVRAS; JANAÍNA GUARIEIRO RIBEIRO DE ASSIS, UNIVERSIDADE FEDERAL DE LAVRAS; NADIA NARA BATISTA, UNIVERSIDADE FEDERAL DE LAVRAS; ANA LUIZA DE OLIVEIRA VILELA, UNIVERSIDADE FEDERAL DE LAVRAS; STTELA DELLYZETE VEIGA F DA ROSA, CNPCA; DISNEY RIBEIRO DIAS, UNIVERSIDADE FEDERAL DE LAVRAS; ROSANE FREITAS SCHWAN, UNIVERSIDADE FEDERAL DE LAVRAS. |
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Título: |
Self-induced anaerobiosis fermentation in coffees inoculated with yeast: effect on key enzymes of the germination process and its relationship with the decrease in seed germination. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Food Research International, 199, 115376, 2025. |
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Páginas: |
9 p. |
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DOI: |
https://doi.org/10.1016/j.foodres.2024.115376 |
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Idioma: |
Inglês |
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Conteúdo: |
Our objective was to monitor the main enzymes of coffee germinal metabolism and chemical composition during Self-Induced Anaerobiosis Fermentation (SIAF) with yeasts (Saccharomyces cerevisiae (CCMA0543), Candida parapsilosis (CCMA0544) and Torulospora delbrueckii (CCMA0684)) evaluating their relationship with seed germination. The starter cultures were assessed by qPCR. The organic acids were analyzed by liquid chromatography. Catalase (CAT), Esterase (EST), Alcohol dehydrogenase (ADH), and Isocitrate Lyase (ICL) enzyme activity was confirmed by the presence of sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-Page) gel bands. The formation of a white halo confirmed the activity of the enzyme endo-β-mannanase, and its quantification was performed using the diameter of the halo of both the samples and the standard curve. At the end of the fermentation process, S. cerevisiae and T. delbrueckii presented the highest populations (>7 log10 cells/g). Succinic acids (average −1.11 g/kg) were consumed during SIAF. Lactic acid increased after 180 h in coffees fermented by the SIAF method (average 3.57 g/kg). CAT and EST showed high activity in the conventional process. ADH activity was detected in both processes after 180 h of the SIAF method. Yeast inoculation during the SIAF method increased the activity of ICL and showed more intense activity in the first 96 h of fermentation, especially the pulped coffee. Endo-β-mannanase activity was intense during conventional coffee processing (9.89–10.99 pmol/min/g). Natural processing tends to preserve a higher percentage of viable seeds. Therefore, the processing and fermentation methods impact seed quality differently. MenosOur objective was to monitor the main enzymes of coffee germinal metabolism and chemical composition during Self-Induced Anaerobiosis Fermentation (SIAF) with yeasts (Saccharomyces cerevisiae (CCMA0543), Candida parapsilosis (CCMA0544) and Torulospora delbrueckii (CCMA0684)) evaluating their relationship with seed germination. The starter cultures were assessed by qPCR. The organic acids were analyzed by liquid chromatography. Catalase (CAT), Esterase (EST), Alcohol dehydrogenase (ADH), and Isocitrate Lyase (ICL) enzyme activity was confirmed by the presence of sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-Page) gel bands. The formation of a white halo confirmed the activity of the enzyme endo-β-mannanase, and its quantification was performed using the diameter of the halo of both the samples and the standard curve. At the end of the fermentation process, S. cerevisiae and T. delbrueckii presented the highest populations (>7 log10 cells/g). Succinic acids (average −1.11 g/kg) were consumed during SIAF. Lactic acid increased after 180 h in coffees fermented by the SIAF method (average 3.57 g/kg). CAT and EST showed high activity in the conventional process. ADH activity was detected in both processes after 180 h of the SIAF method. Yeast inoculation during the SIAF method increased the activity of ICL and showed more intense activity in the first 96 h of fermentation, especially the pulped coffee. Endo-β-mannanase activity was intense during conventional coff... Mostrar Tudo |
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Thesaurus Nal: |
Anaerobiosis; Enzymatic reactions; Fermentation; Seed germination. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02633naa a2200277 a 4500
001 2169579
005 2025-01-09
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.foodres.2024.115376$2DOI
100 1 $aJIMENEZ, E. J. M.
245 $aSelf-induced anaerobiosis fermentation in coffees inoculated with yeast$beffect on key enzymes of the germination process and its relationship with the decrease in seed germination.$h[electronic resource]
260 $c2025
300 $a9 p.
520 $aOur objective was to monitor the main enzymes of coffee germinal metabolism and chemical composition during Self-Induced Anaerobiosis Fermentation (SIAF) with yeasts (Saccharomyces cerevisiae (CCMA0543), Candida parapsilosis (CCMA0544) and Torulospora delbrueckii (CCMA0684)) evaluating their relationship with seed germination. The starter cultures were assessed by qPCR. The organic acids were analyzed by liquid chromatography. Catalase (CAT), Esterase (EST), Alcohol dehydrogenase (ADH), and Isocitrate Lyase (ICL) enzyme activity was confirmed by the presence of sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-Page) gel bands. The formation of a white halo confirmed the activity of the enzyme endo-β-mannanase, and its quantification was performed using the diameter of the halo of both the samples and the standard curve. At the end of the fermentation process, S. cerevisiae and T. delbrueckii presented the highest populations (>7 log10 cells/g). Succinic acids (average −1.11 g/kg) were consumed during SIAF. Lactic acid increased after 180 h in coffees fermented by the SIAF method (average 3.57 g/kg). CAT and EST showed high activity in the conventional process. ADH activity was detected in both processes after 180 h of the SIAF method. Yeast inoculation during the SIAF method increased the activity of ICL and showed more intense activity in the first 96 h of fermentation, especially the pulped coffee. Endo-β-mannanase activity was intense during conventional coffee processing (9.89–10.99 pmol/min/g). Natural processing tends to preserve a higher percentage of viable seeds. Therefore, the processing and fermentation methods impact seed quality differently.
650 $aAnaerobiosis
650 $aEnzymatic reactions
650 $aFermentation
650 $aSeed germination
700 1 $aMARTINS, P. M. M.
700 1 $aASSIS, J. G. R. de
700 1 $aBATISTA, N. N.
700 1 $aVILELA, A. L. de O.
700 1 $aROSA, S. D. V. F. da
700 1 $aDIAS, D. R.
700 1 $aSCHWAN, R. F. S.
773 $tFood Research International, 199, 115376, 2025.
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| 1. |  | ROSÁRIO, A. A. S. e; MELO, A. W. F. de; MAGALHÃES, A. de A.; GOMES, C. V. A.; ROCHA, C. M. F. da; SOUZA, C. M. de; SOARES, D.; ARAÚJO, E. A. de; AMARAL, E. F. do; PANTOJA, E. de S.; SILVA, F. M. e; ALBUQUERQUE, H. S. de; PINHEIRO, J. B.; ALMEIDA, J. S. de; SILVA, J. A. da; GOMES, M. M. da L.; LATUF, M. de O.; ALMEIDA, M. A. Z. de; SILVA, M. M. F. da. Desenvolvimento regional sustentável: Rodovia BR 364, trecho Manuel Urbano-Feijó. Rio Branco, AC: SEMA, 2009. 52 p.| Tipo: Autoria/Organização/Edição de Livros |
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