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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
11/02/2016 |
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Data da última atualização: |
12/02/2016 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
KMIT, M. C. P.; LIMA, A. O. S.; ROMAGNOLI, E. M.; ABDALLA, A. L.; MENDES, R. |
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Afiliação: |
M. C. P. KMIT, ESALQ/USP; A. O. S. LIMA, UNIVALI; E. M. ROMAGNOLI, ESALQ/USP; A. L. ABDALLA, CENA; RODRIGO MENDES, CNPMA. |
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Título: |
Sheep rumen shotgun sequencing for biomass-degrading genes discovery. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 1676-1. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The lignocellulose present in the plant biomass, is a promising source of energy generation. However, the breakdown of plant biomass into simple sugars for bioethanol production is still inefficient and costly due to the recalcitrant nature of plant fiber. Sheep rumen microbiome is specialized in degradation of plant material, but most members of this complex community are non-cultivable in laboratory. Therefore, the search for new lignocellulolytic enzymes in microbial communities naturally evolved in the biomass degradation, in environments such as the rumen, using the exploration of the metagenome, is a promising strategy for the exploration of genes. In this context, this study aimed to obtain plant biomass-degrading genes, selected from the sheep rumen microorganisms. The rumen samples were collected from 6 fistulated animals (Ovis aries), divided into two groups and subjected to two diets: control and sugarcane bagasse, 60 days after the beginning of the experiment. To characterize biomass-degrading genes, the metagenomic DNA was extracted from the solid contents of rumen followed by sequencing in MiSeq Personal Sequencer platform (Illumina®). We analyzed, 4,68 gigabases of metagenomic total DNA from microbes adherent to plant fiber, using on the CLC Genomic Workbench v.5.5.1 platform (CLC Bio, Denmark). The assembled contigs that allowed identification of 27 putative partial carbohydrate-active enzymes (CAE) (NCBI-nr) representing a total of 11 lignocellulases, 13 amylases and 3 other putative CAE from animals fed with control diet and 106 putative partial CAE representing a total of 52 lignocellulases, 46 amylases and 8 other putative CAE from animals fed with diet amended with sugarcane bagasse. These data sets shows the sheep rumen microbiome as an untapped source of potential new fibrolytic enzymes. Using a diet amended with sugarcane bagasse increases the abundance of CAE and provide a substantially expanded catalog of genes participating in the deconstruction of plant biomass MenosAbstract: The lignocellulose present in the plant biomass, is a promising source of energy generation. However, the breakdown of plant biomass into simple sugars for bioethanol production is still inefficient and costly due to the recalcitrant nature of plant fiber. Sheep rumen microbiome is specialized in degradation of plant material, but most members of this complex community are non-cultivable in laboratory. Therefore, the search for new lignocellulolytic enzymes in microbial communities naturally evolved in the biomass degradation, in environments such as the rumen, using the exploration of the metagenome, is a promising strategy for the exploration of genes. In this context, this study aimed to obtain plant biomass-degrading genes, selected from the sheep rumen microorganisms. The rumen samples were collected from 6 fistulated animals (Ovis aries), divided into two groups and subjected to two diets: control and sugarcane bagasse, 60 days after the beginning of the experiment. To characterize biomass-degrading genes, the metagenomic DNA was extracted from the solid contents of rumen followed by sequencing in MiSeq Personal Sequencer platform (Illumina®). We analyzed, 4,68 gigabases of metagenomic total DNA from microbes adherent to plant fiber, using on the CLC Genomic Workbench v.5.5.1 platform (CLC Bio, Denmark). The assembled contigs that allowed identification of 27 putative partial carbohydrate-active enzymes (CAE) (NCBI-nr) representing a total of 11 lignocellulas... Mostrar Tudo |
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Palavras-Chave: |
Lignocelluloytic enzymes; Microbial metagenome; Secind generation biofuel. |
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Thesagro: |
Biotecnologia; Enzima. |
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Thesaurus Nal: |
Metagenomics. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/138802/1/2015RA-065.pdf
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Marc: |
LEADER 02806nam a2200229 a 4500
001 2036656
005 2016-02-12
008 2015 bl uuuu u00u1 u #d
100 1 $aKMIT, M. C. P.
245 $aSheep rumen shotgun sequencing for biomass-degrading genes discovery.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 28., Florianópolis. Anais... Florianópolis: Sociedade Brasileira de Microbiologia, 2015. Ref. 1676-1.$c1676
520 $aAbstract: The lignocellulose present in the plant biomass, is a promising source of energy generation. However, the breakdown of plant biomass into simple sugars for bioethanol production is still inefficient and costly due to the recalcitrant nature of plant fiber. Sheep rumen microbiome is specialized in degradation of plant material, but most members of this complex community are non-cultivable in laboratory. Therefore, the search for new lignocellulolytic enzymes in microbial communities naturally evolved in the biomass degradation, in environments such as the rumen, using the exploration of the metagenome, is a promising strategy for the exploration of genes. In this context, this study aimed to obtain plant biomass-degrading genes, selected from the sheep rumen microorganisms. The rumen samples were collected from 6 fistulated animals (Ovis aries), divided into two groups and subjected to two diets: control and sugarcane bagasse, 60 days after the beginning of the experiment. To characterize biomass-degrading genes, the metagenomic DNA was extracted from the solid contents of rumen followed by sequencing in MiSeq Personal Sequencer platform (Illumina®). We analyzed, 4,68 gigabases of metagenomic total DNA from microbes adherent to plant fiber, using on the CLC Genomic Workbench v.5.5.1 platform (CLC Bio, Denmark). The assembled contigs that allowed identification of 27 putative partial carbohydrate-active enzymes (CAE) (NCBI-nr) representing a total of 11 lignocellulases, 13 amylases and 3 other putative CAE from animals fed with control diet and 106 putative partial CAE representing a total of 52 lignocellulases, 46 amylases and 8 other putative CAE from animals fed with diet amended with sugarcane bagasse. These data sets shows the sheep rumen microbiome as an untapped source of potential new fibrolytic enzymes. Using a diet amended with sugarcane bagasse increases the abundance of CAE and provide a substantially expanded catalog of genes participating in the deconstruction of plant biomass
650 $aMetagenomics
650 $aBiotecnologia
650 $aEnzima
653 $aLignocelluloytic enzymes
653 $aMicrobial metagenome
653 $aSecind generation biofuel
700 1 $aLIMA, A. O. S.
700 1 $aROMAGNOLI, E. M.
700 1 $aABDALLA, A. L.
700 1 $aMENDES, R.
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Registro original: |
Embrapa Meio Ambiente (CNPMA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
29/11/2006 |
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Data da última atualização: |
29/11/2006 |
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Autoria: |
NUNES JÚNIOR, J.; NEIVA, L. C. S.; VIEIRA, N. E.; NUNES, M. R.; MONTEIRO, P. M. F. O.; SILVA, L. O.; TOLEDO, R. M. C. P.; SOUZA, P. I. M.; ASSUNÇÃO, M. da S.; MOREIRA, C. T.; SILVA, S. A. da; FARIAS NETO, A. L. de; YORINORI, J. T.; ALMEIDA, L. A. de; KASTER, M.; KIIHK, R. A. S. |
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Título: |
Cultivar de soja BRSGO-Araçú: comportamento e indicação de cultivo para os estados de Goiás, Distrito Federal, Mato Grosso e Minas Gerais. |
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Ano de publicação: |
2006 |
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Fonte/Imprenta: |
In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 28., 2006, Uberaba. Resumos... Londrina: Embrapa Soja: Fundação Meridional: Fundação Triângulo, 2006. p. 327-328. |
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Idioma: |
Português |
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Thesagro: |
Glycine Max; Soja; Variedade. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01099naa a2200325 a 4500
001 1569985
005 2006-11-29
008 2006 bl uuuu u00u1 u #d
100 1 $aNUNES JÚNIOR, J.
245 $aCultivar de soja BRSGO-Araçú$bcomportamento e indicação de cultivo para os estados de Goiás, Distrito Federal, Mato Grosso e Minas Gerais.
260 $c2006
650 $aGlycine Max
650 $aSoja
650 $aVariedade
700 1 $aNEIVA, L. C. S.
700 1 $aVIEIRA, N. E.
700 1 $aNUNES, M. R.
700 1 $aMONTEIRO, P. M. F. O.
700 1 $aSILVA, L. O.
700 1 $aTOLEDO, R. M. C. P.
700 1 $aSOUZA, P. I. M.
700 1 $aASSUNÇÃO, M. da S.
700 1 $aMOREIRA, C. T.
700 1 $aSILVA, S. A. da
700 1 $aFARIAS NETO, A. L. de
700 1 $aYORINORI, J. T.
700 1 $aALMEIDA, L. A. de
700 1 $aKASTER, M.
700 1 $aKIIHK, R. A. S.
773 $tIn: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 28., 2006, Uberaba. Resumos... Londrina: Embrapa Soja: Fundação Meridional: Fundação Triângulo, 2006. p. 327-328.
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